| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BCR |
| Uniprot No | P11274 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-695aa |
| 氨基酸序列 | MVDPVGFAEA WKAQFPDSEP PRMELRSVGD IEQELERCKA SIRRLEQEVN QERFRMIYLQ TLLAKEKKSY DRQRWGFRRA AQAPDGASEP RASASRPQPA PADGADPPPA EEPEARPDGE GSPGKARPGT ARRPGAAASG ERDDRGPPAS VAALRSNFER IRKGHGQPGA DAEKPFYVNV EFHHERGLVK VNDKEVSDRI SSLGSQAMQM ERKKSQHGAG SSVGDASRPP YRGRSSESSC GVDGDYEDAE LNPRFLKDNL IDANGGSRPP WPPLEYQPYQ SIYVGGMMEG EGKGPLLRSQ STSEQEKRLT WPRRSYSPRS FEDCGGGYTP DCSSNENLTS SEEDFSSGQS SRVSPSPTTY RMFRDKSRSP SQNSQQSFDS SSPPTPQCHK RHRHCPVVVS EATIVGVRKT GQIWPNDGEG AFHGDADGSF GTPPGYGCAA DRAEEQRRHQ DGLPYIDDSP SSSPHLSSKG RGSRDALVSG ALESTKASEL DLEKGLEMRK WVLSGILASE ETYLSHLEAL LLPMKPLKAA ATTSQPVLTS QQIETIFFKV PELYEIHKEF YDGLFPRVQQ WSHQQRVGDL FQKLASQLGV YRAFVDNYGV AMEMAEKCCQ ANAQFAEISE NLRARSNKDA KDPTTKNSLE TLLYKPVDRV TRSTLVLHDL LKHTPASHPD HPLLQDALRI SQNFLSSINE EITPR |
| 预测分子量 | 120 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于BCR(B细胞受体)重组蛋白的经典参考文献，涵盖机制与疾病关联研究：
---
1. **文献名称**：*V(D)J recombination: RAG proteins, repair factors, and regulation*
**作者**：David G. Schatz, Patrick C. Swanson
**摘要**：综述了RAG1/RAG2蛋白在V(D)J重组中的核心作用，解析其如何识别重组信号序列(RSS)并介导DNA双链断裂，同时讨论DNA修复因子(如NHEJ通路)在此过程中的协同机制。
---
2. **文献名称**：*RAG1 and RAG2 form a stable heterodimer and initiate V(D)J recombination via creating double-strand breaks*
**作者**：Martin Gellert et al.
**摘要**：通过生化实验证明RAG1和RAG2形成复合物，直接催化免疫球蛋白基因的DNA切割，阐明了V(D)J重组起始的分子基础及其特异性。
---
3. **文献名称**：*The mechanism of double-strand DNA break repair by the nonhomologous DNA end-joining pathway*
**作者**：Frederick W. Alt, Benjamin P. Sleckman
**摘要**：探讨NHEJ通路在修复RAG诱导的DNA断裂中的关键角色，强调其在BCR多样性生成及基因组稳定性维持中的重要性。
---
4. **文献名称**：*Genetic variants associated with autoimmunity drive B cell receptor signaling and B cell development*
**作者**：Soumya Raychaudhuri et al.
**摘要**：通过全基因组关联分析，揭示BCR重组异常与自身免疫疾病的关联，发现特定基因变异可能通过干扰BCR信号传导或V(D)J重组导致免疫失调。
---
这些文献从分子机制到疾病关联，覆盖了BCR重组蛋白的核心研究领域。
**Background of BCR Recombinant Proteins**
The B-cell receptor (BCR) is a transmembrane protein complex central to adaptive immunity, enabling B cells to recognize antigens and initiate immune responses. Structurally, the BCR consists of a membrane-bound immunoglobulin (mIg) that binds specific antigens and a heterodimeric signaling component, Igα/Igβ (CD79a/CD79b), which transduces intracellular signals. Each B cell expresses a unique BCR, generated through V(D)J recombination—a site-specific DNA rearrangement process during B cell development. This genetic diversification creates a vast repertoire of BCRs, allowing recognition of diverse pathogens.
BCR recombinant proteins are engineered versions of these components, produced using recombinant DNA technology. They are pivotal in studying BCR structure-function relationships, antigen-binding specificity, and downstream signaling mechanisms. For instance, recombinant mIg fragments or soluble BCRs are expressed in mammalian, insect, or bacterial systems to investigate antigen interactions or develop diagnostic tools. Additionally, Igα/Igβ cytoplasmic domains are often fused to reporter systems to dissect signaling pathways involving kinases like Syk, BTK, or PI3K.
In therapeutic contexts, BCR recombinant proteins aid in designing monoclonal antibodies, bispecific antibodies, or chimeric antigen receptors (CARs) for cancer immunotherapy. They also model autoimmune diseases caused by aberrant BCR signaling, such as systemic lupus erythematosus (SLE), facilitating drug screening. Furthermore, recombinant BCRs help identify neoantigens in B cell malignancies, guiding targeted therapies.
Overall, BCR recombinant proteins bridge basic immunology and translational research, offering tools to decode immune activation, develop biologics, and address B cell-related pathologies. Their versatility underscores their importance in advancing immunotherapies and precision medicine.
×
