Recombinant Human FGF3 protein

中文名： 成纤维细胞生长因子3(FGF3)重组蛋白
别名： FGF3；INT2；Fibroblast growth factor 3

货号: PA1000-8188

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	FGF3
Uniprot No	P11487
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	18-239aa
氨基酸序列	AAGPGARLRRDAGGRGGVYEHLGGAPRRRKLYCATKYHLQLHPSGRVNGSLENSAYSILEITAVEVGIVAIRGLFSGRYLAMNKRGRLYASEHYSAECEFVERIHELGYNTYASRLYRTVSSTPGARRQPSAERLWYVSVNGKGRPRRGFKTRRTQKSSLFLPRVLDHRDHEMVRQLQSGLPRPPGKGVQPRRRRQKQSPDNLEPSHVQASRLGSQLEASAH
预测分子量	32.4 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于FGF3重组蛋白的3篇参考文献摘要示例(文献信息为虚构，仅供格式参考)：

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1. **标题**: "Recombinant Human FGF3: Production and Functional Characterization in Epithelial Morphogenesis"

**作者**: Smith A, et al.

**摘要**: 研究通过大肠杆菌表达系统成功制备重组人FGF3蛋白，并验证其诱导哺乳动物上皮细胞迁移和分支形态发生的能力，证实其通过FGFR2受体激活MAPK通路。

2. **标题**: "Structural Analysis of FGF3 Reveals Key Residues for Receptor Binding"

**作者**: Lee J, et al.

**摘要**: 利用X射线晶体学解析重组FGF3蛋白的三维结构，发现其β-trefoil结构域中特定氨基酸残基对FGFR1结合至关重要，为靶向药物设计提供依据。

3. **标题**: "FGF3 Recombinant Protein Promotes Hair Follicle Regeneration in Murine Models"

**作者**: Chen X, et al.

**摘要**: 在小鼠模型中验证重组FGF3蛋白通过激活Wnt/β-catenin信号通路促进毛囊干细胞增殖，表明其在脱发治疗中的潜在应用价值。

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提示：实际文献需通过PubMed或Google Scholar检索关键词“recombinant FGF3”、“FGF3 protein function”获取。

背景信息

Fibroblast Growth Factor 3 (FGF3) is a member of the FGF family, a group of signaling proteins involved in diverse biological processes such as cell proliferation, differentiation, angiogenesis, and embryonic development. Initially identified through homology with other FGFs, FGF3 plays critical roles in tissue repair, organogenesis, and patterning during embryogenesis. It is particularly associated with craniofacial development, inner ear morphogenesis, and hair follicle cycling. Dysregulation of FGF3 has been linked to developmental disorders and cancers, including breast and thyroid carcinomas, underscoring its importance in both normal physiology and disease.

Recombinant FGF3 protein is engineered using genetic recombination techniques, typically expressed in prokaryotic (e.g., E. coli) or eukaryotic systems (e.g., mammalian or insect cells) to ensure proper folding and post-translational modifications. This bioengineered form retains the biological activity of native FGF3. enabling researchers to study its mechanisms without relying on animal-derived sources. Its production involves cloning the FGF3 gene into expression vectors, followed by purification via chromatography methods like affinity or ion-exchange.

In research, recombinant FGF3 is used to investigate signaling pathways, particularly its interaction with FGF receptors (FGFRs) and heparan sulfate proteoglycans, which are essential for signal transduction. It also serves as a tool in regenerative medicine, aiding studies on tissue repair, stem cell differentiation, and organoid development. Therapeutic applications are being explored, though challenges like stability, delivery, and off-target effects remain. Overall, recombinant FGF3 provides a controlled, scalable resource for advancing both basic science and translational research in developmental biology and oncology.