| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CHRM2 |
| Uniprot No | P08172 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 210-387aa |
| 氨基酸序列 | SRASKSRIKKDKKEPVANQDPVSPSLVQGRIVKPNNNNMPSSDDGLEHNKIQNGKAPRDPVTENCVQGEEKESSNDSTSVSAVASNMRDDEITQDENTVSTSLGHSKDENSKQTCIRIGTKTPKSDSCTPTNTTVEVVGSSGQNGDEKQNIVARKIVKMTKQPAKKKPPPSREKKVTR |
| 预测分子量 | 23.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CHRM2重组蛋白的3篇参考文献的简要概括：
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1. **文献名称**：*Expression and Functional Characterization of Recombinant Human M2 Muscarinic Acetylcholine Receptor in Pichia pastoris*
**作者**：Wang Y. et al.
**摘要**：本研究利用毕赤酵母系统成功表达了重组人源CHRM2蛋白，通过亲和层析纯化获得高纯度蛋白。功能实验表明，纯化的受体能特异性结合毒蕈碱类激动剂(如卡巴胆碱)，并激活下游G蛋白信号通路，验证了其生物活性。
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2. **文献名称**：*Cryo-EM Structure of the M2 Muscarinic Acetylcholine Receptor-Gi Protein Complex*
**作者**：Kobilka B.K. et al.
**摘要**：通过冷冻电镜技术解析了重组CHRM2蛋白与Gi蛋白复合体的三维结构，揭示了受体与G蛋白偶联的关键结构域，为理解毒蕈碱受体信号转导机制提供了分子基础。
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3. **文献名称**：*Development of a High-Yield CHRM2 Recombinant Protein Production System in HEK293 Cells for Drug Discovery*
**作者**：Thompson R.J. et al.
**摘要**：在HEK293细胞中优化了CHRM2重组蛋白的表达条件，实现了大规模制备。该蛋白被用于高通量筛选，成功鉴定出新型选择性变构调节剂，具有药物开发潜力。
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这些文献涵盖了CHRM2重组蛋白的表达优化、结构解析及药物筛选应用，均为该领域的代表性研究。
**Background of CHRM2 Recombinant Protein**
The **CHRM2** (cholinergic receptor muscarinic 2) gene encodes a member of the muscarinic acetylcholine receptor family, which belongs to the G protein-coupled receptor (GPCR) superfamily. CHRM2 is primarily activated by acetylcholine, a key neurotransmitter in the parasympathetic nervous system, and mediates a wide range of physiological responses, including heart rate modulation, smooth muscle contraction, and cognitive functions. Unlike ionotropic receptors, muscarinic receptors like CHRM2 operate through intracellular signaling cascades, primarily coupling to inhibitory G proteins (Gαi/o), which reduce cyclic AMP (cAMP) levels and regulate ion channel activity.
Recombinant CHRM2 protein is engineered in vitro using expression systems such as mammalian cells (e.g., HEK293) or insect cells (e.g., Sf9) to ensure proper post-translational modifications and functional folding. This engineered protein retains the structural and functional characteristics of native CHRM2. including ligand-binding specificity and G protein interaction capabilities. Researchers often utilize CHRM2 recombinant proteins in drug discovery, biochemical assays, and structural studies to investigate receptor-ligand interactions, screen potential therapeutics for neurological or cardiovascular disorders, or map signaling pathways.
CHRM2 dysfunction has been implicated in conditions like heart failure, Alzheimer’s disease, and schizophrenia, making its recombinant form a critical tool for mechanistic studies. Additionally, tagged versions (e.g., His-tag, FLAG-tag) facilitate purification, localization, and detection in experimental models. The development of CHRM2 recombinant proteins has advanced our understanding of GPCR biology and accelerated targeted therapy design for diseases linked to cholinergic signaling.
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