| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PAR2 |
| Uniprot No | O75469 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-434aa |
| 氨基酸序列 | MEVRPKESWNHADFVHCEDTESVPGKPSVNADEEVGGPQICRVCGDKATGYHFNVMTCEGCKGFFRRAMKRNARLRCPFRKGACEITRKTRRQCQACRLRKCLESGMKKEMIMSDEAVEERRALIKRKKSERTGTQPLGVQGLTEEQRMMIRELMDAQMKTFDTTFSHFKNFRLPGVLSSGCELPESLQAPSREEAAKWSQVRKDLCSLKVSLQLRGEDGSVWNYKPPADSGGKEIFSLLPHMADMSTYMFKGIISFAKVISYFRDLPIEDQISLLKGAAFELCQLRFNTVFNAETGTWECGRLSYCLEDTAGGFQQLLLEPMLKFHYMLKKLQLHEEEYVLMQAISLFSPDRPGVLQHRVVDQLQEQFAITLKSYIECNRPQPAHRFLFLKIMAMLTELRSINAQHTQRLLRIQDIHPFATPLMQELFGITGS |
| 预测分子量 | 69.8kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Protease-activated receptor 2 crystal structures for structure-based drug discovery"** by Cheng et al. (2020)
摘要：报道了PAR2的高分辨率晶体结构，揭示了其配体结合口袋的构象变化，为基于结构的拮抗剂设计提供依据。
2. **"Recombinant expression and functional characterization of protease-activated receptor 2 in inflammatory signaling"** by Ramachandran et al. (2012)
摘要：通过哺乳动物细胞系统重组表达PAR2.阐明其被胰蛋白酶激活后触发NF-κB通路并促进炎症因子释放的机制。
3. **"Development of a PAR2-targeted fluorescent probe using recombinant extracellular domain mutants"** by Adams et al. (2018)
摘要：利用大肠杆菌表达体系制备PAR2胞外域突变体重组蛋白，开发出选择性荧光探针，用于实时监测受体激活状态。
4. **"PAR2 recombinant protein-based screening identifies novel small molecule antagonists for cancer therapy"** by Kelso et al. (2016)
摘要：通过重组PAR2蛋白的高通量筛选，发现新型拮抗剂可抑制肿瘤细胞迁移和血管生成，具有潜在抗癌应用价值。
(注：以上文献信息为模拟示例，实际引用请核对真实出版物。)
**Background of PAR2 Recombinant Protein**
Protease-activated receptor 2 (PAR2), a member of the G protein-coupled receptor (GPCR) family, is uniquely activated by proteolytic cleavage. Unlike traditional ligand-binding receptors, PAR2 is triggered when extracellular proteases (e.g., trypsin, mast cell tryptase) cleave its N-terminal domain, exposing a tethered ligand that binds intramolecularly to initiate downstream signaling. This mechanism links PAR2 to diverse physiological and pathological processes, including inflammation, pain, tissue repair, and cancer.
PAR2 is widely expressed in epithelial, endothelial, immune, and neuronal cells, reflecting its multifaceted roles. It activates pathways such as MAPK, NF-κB, and PI3K/AKT, modulating immune responses, cell proliferation, and barrier function. Dysregulation of PAR2 is implicated in chronic inflammatory diseases (e.g., arthritis, colitis), allergic disorders, neuropathic pain, and cancer progression, where it often promotes pro-inflammatory or pro-survival signals.
PAR2 recombinant protein, produced via genetic engineering in systems like *E. coli* or mammalian cells, retains the receptor’s functional domains for research applications. This protein is pivotal in studying PAR2’s structure-function relationships, ligand interactions, and signaling mechanisms. It also aids in drug discovery, serving as a target for screening therapeutic inhibitors or agonists. For instance, PAR2 antagonists are explored for treating inflammation and pain, while its role in cancer metastasis highlights its potential as a biomarker or therapeutic target.
Despite its promise, PAR2 research faces challenges, including receptor complexity, biased signaling, and species-specific differences. Recombinant PAR2 tools help address these issues, enabling precise mechanistic studies and advancing translational applications in personalized medicine.
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