| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LRP1 |
| Uniprot No | Q07954-2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-292aa |
| 氨基酸序列 | MLTPPLLLLLPLLSALVAAAIDAPKTCSPKQFACRDQITCISKGWRCDGE RDCPDGSDEAPEICPQSKAQRCQPNEHNCLGTELCVPMSRLCNGVQDCMD GSDEGPHCRELQGNCSRLGCQHHCVPTLDGPTCYCNSSFQLQADGKTCKD FDECSVYGTCSQLCTNTDGSFICGCVEGYLLQPDNRSCKAKNEPVDRPPV LLIANSQNILATYLSGAQVSTITPTSTRQTTAMDFSYANETVCWVHVGDS AAQTQLKCARMPGLKGFVDEHTINISLSLHLCVFSKSQQEMG |
| 预测分子量 | 58 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与LRP1重组蛋白相关的文献示例(内容基于领域内典型研究方向概括，具体文献需通过数据库验证)：
1. **文献名称**：*"Recombinant expression and functional characterization of the LDL receptor-related protein 1 (LRP1) extracellular domain"*
**作者**：Smith J. et al.
**摘要**：研究利用哺乳动物细胞(HEK293)成功表达并纯化LRP1的胞外结构域重组蛋白，验证其与配体(如Aβ、α2M)的结合活性，为研究LRP1介导的细胞内吞机制提供工具。
2. **文献名称**：*"LRP1 recombinant protein attenuates amyloid-β toxicity in Alzheimer's disease models"*
**作者**：Zhang Y. et al.
**摘要**：通过昆虫细胞表达系统制备可溶性LRP1重组蛋白，证明其能结合并清除Aβ寡聚体，减轻神经元毒性，提示其潜在治疗阿尔茨海默病的应用价值。
3. **文献名称**：*"Structural insights into LRP1-mediated signaling through recombinant domain mapping"*
**作者**：Garcia-Reyes M. et al.
**摘要**：利用重组表达的不同LRP1功能域蛋白(如Cluster II、IV)，结合晶体学分析揭示其与信号分子(如PDGF、TGF-β)的相互作用位点，阐明LRP1调控细胞增殖的分子机制。
4. **文献名称**：*"High-yield purification of functional LRP1 ectodomain using a baculovirus-insect cell system"*
**作者**：Lee S. & Kim T.
**摘要**：优化杆状病毒-昆虫细胞表达体系，实现LRP1胞外域重组蛋白的高效分泌表达，并通过生物膜干涉技术(BLI)定量分析其与多种配体的结合动力学参数。
**注**：以上为模拟摘要，实际文献需通过PubMed/Google Scholar检索关键词“LRP1 recombinant protein”或“LRP1 ectodomain expression”获取。
**Background of LRP1 Recombinant Protein**
Low-density lipoprotein receptor-related protein 1 (LRP1), also known as CD91 or α2-macroglobulin receptor, is a multifunctional transmembrane receptor belonging to the LDL receptor family. Initially identified for its role in lipoprotein metabolism, LRP1 has since been recognized as a critical player in diverse cellular processes, including endocytosis, signal transduction, cell migration, and tissue homeostasis. It interacts with over 40 ligands, such as apolipoprotein E, proteases, growth factors, and extracellular matrix components, regulating pathways linked to inflammation, coagulation, and neurodegeneration. Dysregulation of LRP1 is implicated in diseases like Alzheimer’s disease, atherosclerosis, and cancer, underscoring its therapeutic and diagnostic relevance.
Recombinant LRP1 proteins are engineered using expression systems (e.g., mammalian, insect, or bacterial cells) to produce specific domains or full-length variants for research. These proteins retain functional epitopes, enabling studies on ligand-receptor interactions, structural analysis, and mechanism elucidation. For instance, soluble LRP1 (sLRP1) fragments are often generated to investigate extracellular domain functions, while truncated forms help map binding sites. Recombinant LRP1 is vital for developing targeted therapies, such as blocking pathogenic ligand uptake or modulating signaling cascades in cancer. Additionally, it serves as a tool for antibody development and biomarker discovery. By providing a controlled, pure protein source, recombinant LRP1 accelerates both basic research and translational applications, bridging gaps in understanding its complex roles in health and disease.
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