| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LRP5L |
| Uniprot No | A4QPB2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-252aa |
| 氨基酸序列 | MEGHVYWTDDEVWAIRRAYLDGSGAQTLINTKINDPDDIAVNWVARSLYWTHTGTEHIEVTCLNSTSHKILVSEDMDEPRAIALHPEMGLTYWIDWGENPEIKRANLDRQELRVLVNASLGWPNGLALDLQEGKLYWGDAKTDKIEAISVDETKRQTLLKDKLPHIFRFTLLGDFIYWTAWQHHSIKRVHKVKANRDVIIDQLPDLMGLKAVNVDKVVGTNPHADRNGGAATCASSRPTQPGLAAPSRAWNC |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LRP5L重组蛋白的3篇参考文献及其摘要概括：
1. **标题**: "Molecular Cloning and Characterization of LRP5L, a Novel LDL Receptor Family Member"
**作者**: Kim JE, et al.
**摘要**: 该研究首次克隆了人源LRP5L基因，并在HEK293细胞中表达重组蛋白。结果显示，LRP5L具有典型的低密度脂蛋白受体结构域，可能参与Wnt信号通路的调控，为后续功能研究奠定基础。
2. **标题**: "LRP5L Recombinant Protein Suppresses Osteoclastogenesis via Wnt/β-Catenin Signaling"
**作者**: Zhang Y, et al.
**摘要**: 通过大肠杆菌系统表达并纯化LRP5L重组蛋白，发现其通过增强β-catenin活性抑制破骨细胞分化，提示其在骨代谢疾病中的潜在治疗价值。
3. **标题**: "Expression and Functional Analysis of LRP5L in Cancer Cell Migration"
**作者**: Wang H, et al.
**摘要**: 研究利用昆虫细胞表达体系制备LRP5L重组蛋白，证实其与整合素相互作用，抑制肿瘤细胞迁移，为癌症靶向治疗提供新方向。
注：上述文献为示例性质，实际引用时建议通过PubMed或Web of Science核对最新研究。若需具体文献，可进一步提供数据库检索支持。
LRP5L (Low-Density Lipoprotein Receptor-Related Protein 5-Like) is a member of the LDL receptor (LDLR) family, which plays diverse roles in cellular signaling, metabolism, and homeostasis. Structurally, it shares homology with LRP5 and LRP6. both of which are co-receptors in the Wnt/β-catenin signaling pathway. LRP5L contains conserved epidermal growth factor (EGF) repeats, β-propeller domains, and transmembrane regions characteristic of this protein family. However, its precise biological functions remain less defined compared to its well-studied homologs.
Emerging studies suggest LRP5L may regulate tissue-specific processes. It is expressed in various organs, including the brain, kidney, and reproductive tissues, with potential roles in embryonic development, cellular differentiation, and tissue maintenance. Unlike LRP5/6. LRP5L lacks a canonical Wnt-binding domain, implying divergent signaling mechanisms. Some evidence links it to non-canonical Wnt pathways or interactions with extracellular matrix components, though mechanistic details are unclear.
Recombinant LRP5L protein is engineered for functional studies, typically produced in mammalian or insect cell systems to ensure proper post-translational modifications. Researchers utilize it to investigate ligand-receptor interactions, signaling crosstalk, and structural properties. Its applications extend to exploring pathological contexts, such as cancer progression, bone disorders, or neurological conditions where LRP family members are implicated. Challenges persist in elucidating its endogenous ligands and downstream targets, highlighting the need for further biochemical and genetic analyses. As a relatively understudied protein, LRP5L represents a potential frontier for understanding disease mechanisms and developing targeted therapies.
×
