| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DIO1 |
| Uniprot No | Q9BTC0-2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-544aa |
| 氨基酸序列 | MDDKGDPSNEEAPKAIKPTSKEFRKTWGFRRTTIAKREGAGDAEADPLEP PPPQQQLGLSLRRSGRQPKRTERVEQFLTIARRRGRRSMPVSLEDSGEPT SCPATDAETASEGSVESASETRSGPQSASTAVKERPASSEKVKGGDDHDD TSDSDSDGLTLKELQNRLRRKREQEPTERPLKGIQSRLRKKRREEGPAET VGSEASDTVEGVLPSKQEPENDQGVVSQAGKDDRESKLEGKAAQDIKDEE PGDLGRPKPECEGYDPNALYCICRQPHNNRFMICCDRCEEWFHGDCVGIS EARGRLLERNGEDYICPNCTILQVQDETHSETADQQEAKWRPGDADGTDC TSIGTIEQKSSEDQGIKGRIEKAANPSGKKKLKIFQPVIEAPGASKCIGP GCCHVAQPDSVYCSNDCILKHAAATMKFLSSGKEQKPKPKEKMKMKPEKP SLPKCGAQAGIKISSVHKRPAPEKKETTVKKAVVVPARSEALGKEAACES STPSWASDHNYNAVKPEKTAAPSPSLLYKCSGKYLYSLHPSLIA |
| 预测分子量 | 86 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DIO1重组蛋白的3篇参考文献示例(文献为虚构示例，仅作格式参考)：
1. **文献名称**：*Recombinant Human DIO1: Expression, Purification, and Enzymatic Characterization*
**作者**：Smith A, et al.
**摘要**：研究报道了通过大肠杆菌系统重组表达人源DIO1蛋白的优化方法，采用His标签纯化获得高活性蛋白，并验证其催化T4向T3转化的酶动力学参数。
2. **文献名称**：*Structural Insights into DIO1 Function via Crystallographic Analysis of Recombinant Protein*
**作者**：Chen L, et al.
**摘要**：利用重组表达的DIO1蛋白进行X射线晶体结构解析，揭示了其底物结合域的关键氨基酸残基，为甲状腺激素代谢机制提供分子基础。
3. **文献名称**：*DIO1 Recombinant Protein Modulates Thyroid Hormone Homeostasis in Cellular Models*
**作者**：Wang Y, et al.
**摘要**：通过体外细胞实验证明，外源性添加重组DIO1蛋白可增强细胞内T3水平，并逆转甲状腺功能减退模型的病理表型，提示其潜在治疗应用价值。
(注：以上文献为模拟内容，实际文献需通过PubMed或SciHub等平台检索。)
**Background of DIO1 Recombinant Protein**
Deiodinase iodothyronine type 1 (DIO1) is a selenoenzyme critical in regulating thyroid hormone homeostasis by catalyzing the activation and inactivation of thyroid hormones. It primarily converts the prohormone thyroxine (T4) into the biologically active triiodothyronine (T3) through outer-ring deiodination, while also degrading T3 and T4 into inactive metabolites via inner-ring deiodination. This dual function positions DIO1 as a key modulator of systemic and local thyroid hormone availability, influencing metabolic processes, development, and energy balance.
The recombinant DIO1 protein is engineered to mimic the native enzyme’s structure and function, typically expressed in heterologous systems like *E. coli*, yeast, or mammalian cells. Recombinant technology allows for high-purity, scalable production of DIO1. enabling detailed biochemical and pharmacological studies. Its applications span thyroid disorder research (e.g., hypothyroidism, hyperthyroidism), metabolic disease modeling, and drug discovery targeting thyroid-related pathways. Additionally, recombinant DIO1 facilitates investigations into selenium’s role in enzyme activity, as the selenocysteine residue in its active site is essential for catalytic function.
Research leveraging recombinant DIO1 has advanced understanding of tissue-specific thyroid hormone regulation and its implications in conditions like obesity, cardiovascular diseases, and cancer. It also serves as a tool for screening deiodinase inhibitors or activators, potentially guiding therapeutic strategies. Despite its importance, DIO1’s activity varies across species and tissues, emphasizing the need for species-specific recombinant proteins in translational studies. Overall, recombinant DIO1 remains indispensable for unraveling thyroid hormone dynamics and developing targeted therapies.
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