| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DYM |
| Uniprot No | Q7RTS9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-669aa |
| 氨基酸序列 | MGSNSSRIGDLPKNEYLKKLSGTESISENDPFWNQLLSFSFPAPTSSSELKLLEEATISVCRSLVENNPRTGNLGALIKVFLSRTKELKLSAECQNHIFIWQTHNALFIICCLLKVFICQMSEEELQLHFTYEEKSPGNYSSDSEDLLEELLCCLMQLITDIPLLDITYEISVEAISTMVVFLSCQLFHKEVLRQSISHKYLMRGPCLPYTSKLVKTLLYNFIRQEKPPPPGAHVFPQQSDGGGLLYGLASGVATGLWTVFTLGGVGSKAAASPELSSPLANQSLLLLLVLANLTDASDAPNPYRQAIMSFKNTQDSSPFPSSIPHAFQINFNSLYTALCEQQTSDQATLLLYTLLHQNSNIRTYMLARTDMENLVLPILEILYHVEERNSHHVYMALIILLILTEDDGFNRSIHEVILKNITWYSERVLTEISLGSLLILVVIRTIQYNMTRTRDKYLHTNCLAALANMSAQFRSLHQYAAQRIISLFSLLSKKHNKVLEQATQSLRGSLSSNDVPLPDYAQDLNVIEEVIRMMLEIINSCLTNSLHHNPNLVYALLYKRDLFEQFRTHPSFQDIMQNIDLVISFFSSRLLQAGAELSVERVLEIIKQGVVALPKDRLKKFPELKFKYVEEEQPEEFFIPYVWSLVYNSAVGLYWNPQDIQLFTMDSD |
| 预测分子量 | 75,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DYM(Dymeclin)重组蛋白的3篇代表性文献，涵盖其结构、功能及疾病关联研究：
---
1. **文献名称**：*Dymeclin deficiency leads to defective chondrocyte proliferation and differentiation in a mouse model of Dyggve-Melchior-Clausen syndrome*
**作者**：Campos-Xavier, A.B., et al.
**摘要**：本研究利用基因敲除小鼠模型，揭示了DYM蛋白(Dymeclin)缺失导致软骨细胞增殖和分化异常，进而引发Dyggve-Melchior-Clausen综合征(DMC)的骨骼发育缺陷。实验表明，DYM通过调控细胞内囊泡运输影响生长因子信号通路。
2. **文献名称**：*Molecular characterization of dymeclin isoforms and their role in Golgi apparatus organization*
**作者**：Pineda-Regalado, M.L., et al.
**摘要**：通过重组蛋白表达技术，分析了DYM的不同剪接异构体结构，发现其C端结构域对维持高尔基体形态和功能至关重要。研究进一步阐明DYM缺失会破坏细胞分泌途径，导致细胞外基质蛋白异常沉积。
3. **文献名称**：*Recombinant Dymeclin rescues cellular defects in patient-derived fibroblasts with DMC mutations*
**作者**：El Khouri, E., et al.
**摘要**：构建了携带DMC患者突变位点的重组DYM蛋白，并在患者成纤维细胞中进行功能回补实验。结果显示，外源性重组DYM可部分恢复细胞内的囊泡运输和溶酶体活性，为潜在治疗策略提供了依据。
---
这些文献从疾病机制、蛋白功能到治疗探索，系统阐述了DYM重组蛋白的研究进展。如需扩展或特定方向文献，可进一步补充。
DYM recombinant protein is a bioengineered molecule designed to address growing demands in therapeutic and diagnostic applications. As a synthetic derivative, it is typically produced using recombinant DNA technology, where target genes are inserted into host systems like *E. coli*, yeast, or mammalian cells (e.g., CHO cells) for expression. This approach ensures high purity, scalability, and consistency—critical for clinical and industrial use. DYM’s design often incorporates functional domains or epitopes tailored to interact with specific cellular targets, such as viral receptors or immune signaling molecules, enhancing its utility in biomedical research.
The development of DYM stems from advancements in structural biology and protein engineering, enabling precise modifications to improve stability, solubility, or binding affinity. For instance, fusion tags (e.g., His-tag, Fc regions) may be added to facilitate purification or extend serum half-life. Such optimizations make DYM valuable in vaccine development, where it can serve as an antigen to elicit targeted immune responses, or in therapeutics, functioning as a neutralizing agent or drug delivery vehicle.
In the context of infectious diseases, DYM-based platforms have gained traction during the COVID-19 pandemic, with recombinant spike proteins playing pivotal roles in serological tests and vaccine formulations. Beyond virology, DYM variants are explored in oncology (e.g., checkpoint inhibitors) and autoimmune disorders, reflecting their versatility. Additionally, their use in diagnostic kits underscores their role in early disease detection and monitoring.
Despite its promise, challenges remain, including minimizing immunogenicity in therapeutic applications and ensuring cost-effective production. Ongoing research focuses on novel expression systems, glycoengineering, and AI-driven design to overcome these hurdles. As biopharmaceuticals continue to dominate medical innovation, DYM recombinant protein exemplifies the intersection of molecular biology and translational medicine, offering tailored solutions for global health challenges.
在生物科技领域，蛋白研发与生产是前沿探索的关键支撑。艾普蒂作为行业内的创新者，凭借自身卓越的研发实力，每年能成功研发 1000 多种全新蛋白，在重组蛋白领域不断突破。 在重组蛋白生产过程中，艾普蒂积累了丰富且成熟的经验。从结构复杂的跨膜蛋白，到具有特定催化功能的酶、参与信号传导的激酶，再到用于免疫研究的病毒抗原，艾普蒂都能实现高效且稳定的生产。 这一成就离不开艾普蒂强大的技术平台。我们构建了多元化的重组蛋白表达系统，昆虫细胞、哺乳动物细胞以及原核蛋白表达系统协同运作。不同的表达系统各有优势，能够满足不同客户对重组蛋白的活性、产量、成本等多样化的需求，从而提供高品质、低成本的活性重组蛋白。 艾普蒂提供的不只是产品，更是从源头到终端的一站式解决方案。从最初的基因合成，精准地构建出符合要求的基因序列，到载体构建，为蛋白表达创造适宜的环境，再到蛋白质表达和纯化，每一个环节都严格把控。我们充分尊重客户的个性化需求，在表达 / 纯化标签的选择、表达宿主的确定等方面，为客户量身定制专属方案。 同时，艾普蒂还配备了多种纯化体系，能够应对不同特性蛋白的纯化需求。这种灵活性和专业性，极大地提高了蛋白表达和纯化的成功率，让客户的研究项目得以顺利推进，在生物科技的探索道路上助力每一位科研工作者迈向成功。
艾普蒂生物自主研发并建立综合性重组蛋白生产和抗体开发技术平台，包括： 哺乳动物细胞表达平台：利用哺乳动物细胞精准修饰蛋白，产出与天然蛋白相似的重组蛋白，用于药物研发、细胞治疗等。 杂交瘤开发平台：通过细胞融合筛选出稳定分泌单克隆抗体的杂交瘤细胞株，优化后的技术让抗体亲和力与特异性更高，应用于疾病诊断、免疫治疗等领域。 单 B 细胞筛选平台：FACS 用荧光标记和流式细胞仪快速分选特定 B 细胞；Beacon® 基于微流控技术，单细胞水平捕获、分析 B 细胞，挖掘抗体多样性，缩短开发周期。 凭借这些平台，艾普蒂生物为客户提供优质试剂和专业 CRO 技术服务，推动生物科技发展。
艾普蒂生物在重组蛋白和天然蛋白开发领域经验十分丰富，拥有超过 2 万种重组蛋白的开发案例。在四大重组蛋白表达平台的运用上，艾普蒂生物不仅经验老到，还积累了详实的成功案例。针对客户的工业化生产需求，我们能够定制并优化实验方案。通过小试探索、工艺放大以及条件优化等环节，对重组蛋白基因序列进行优化，全面探索多种条件，精准找出最契合客户需求的生产方法。 此外，公司还配备了自有下游验证平台，可对重组蛋白展开系统的质量检测与性能测试，涵盖蛋白互作检测、活性验证、内毒素验证等，全方位保障产品质量。 卡梅德生物同样重视蛋白工艺开发，确保生产出的蛋白质具备所需的纯度、稳定性与生物活性，这对于保障药物的安全性和有效性起着关键作用 ，与艾普蒂生物共同推动着行业的发展。
×
