关键信息
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基因名
NEU3
- 应用
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别名
NEU3；NEURL3；E3 ubiquitin-Protein ligase NEURL1B
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9UQ49
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表达区间
2-428aa
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氨基酸序列
EEVTTCSFNSPLFRQEDDRGITYRIPALLYIPPTHTFLAFAEKRSTRRDEDALHLVLRRGLRIGQLVQWGPLKPLMEATLPGHRTMNPCPVWEQKSGCVFLFFICVRGHVTERQQIVSGRNAARLCFIYSQDAGCSWSEVRDLTEEVIGSELKHWATFAVGPGHGIQLQSGRLVIPAYTYYIPSWFFCFQLPCKTRPHSLMIYSDDLGVTWHHGRLIRPMVTVECEVAEVTGRAGHPVLYCSARTPNRCRAEALSTDHGEGFQRLALSRQLCEPPHGCQGSVVSFRPLEIPHRCQDSSSKDAPTIQQSSPGSSLRLEEEAGTPSESWLLYSHPTSRKQRVDLGIYLNQTPLEAACWSRPWILHCGPCGYSDLAALEEEGLFGCLFECGTKQECEQIAFRLFTHREILSHLQGDCTSPGRNPSQFKSN
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分子量
52.1 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
NEU3, a sialidase enzyme, plays a crucial role in the metabolism of sialic acids, which are important components of glycoproteins and glycolipids on cell surfaces. Research has shown that NEU3 is involved in various physiological processes, including cell adhesion, immune response, and signaling pathways. Its dysregulation has been linked to several pathological conditions, such as cancer, where altered sialic acid expression can influence tumor progression and metastasis. The study of NEU3 recombinant protein has gained significant attention in recent years due to its potential therapeutic implications. By producing NEU3 in a recombinant form, researchers can investigate its enzymatic activity, substrate specificity, and interactions with other cellular components in detail. This understanding could pave the way for novel cancer treatments or interventions in diseases associated with sialic acid metabolism. Furthermore, NEU3's role in modulating cell signaling makes it a valuable target for drug development, as restoring its normal function may help counteract some of the damaging effects seen in disease states. Thus, the exploration of NEU3 recombinant protein not only enhances our basic understanding of sialidase functions but also opens avenues for innovative therapeutic strategies in oncology and other fields aligned with aberrant sialic acid biology.
