关键信息
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基因名
WDR54
- 应用
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别名
WD repeat-containing Protein 54; WDR54; WDR54_HUMAN
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9H977
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表达区间
1-334 aa
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氨基酸序列
MFRWERSIPL RGSAAALCNN LSVLQLPARN LTYFGVVHGP SAQLLSAAPE GVPLAQRQLH AKEGAGVSPP LITQVHWCVL PFRVLLVLTS HRGIQMYESN GYTMVYWHAL DSGDASPVQA VFARGIAASG HFICVGTWSG RVLVFDIPAK GPNIVLSEEL AGHQMPITDI ATEPAQGQDC VADMVTADDS GLLCVWRSGP EFTLLTRIPG FGVPCPSVQL WQGIIAAGYG NGQVHLYEAT TGNLHVQINA HARAICALDL ASEVGKLLSA GEDTFVHIWK LSRNPESGYI EVEHCHGECV ADTQLCGARF CDSSGNSFAV TGYDLAEIRR FSSV
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分子量
35.8 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
WDR54, a member of the WD-repeat protein family, has garnered attention in recent years due to its potential roles in various cellular processes, including RNA processing, cell cycle regulation, and stress responses. Research indicates that WDR54 may play a crucial role in mitochondrial function and the regulation of apoptosis, making it a gene of interest in studies related to cancer and neurodegenerative diseases. The functionality of WDR54 is further underscored by its involvement in the assembly of multi-protein complexes, which are vital for the proper functioning of cellular machinery. However, despite the accumulating evidence of its biological significance, the precise mechanisms of action and the pathway interactions of WDR54 remain insufficiently characterized. This has prompted researchers to focus on the production of recombinant WDR54 protein to facilitate detailed biochemical analyses and structural studies. By utilizing recombinant protein techniques, scientists aim to elucidate WDR54's role within the cell, investigate its interaction with other proteins, and assess its potential as a therapeutic target. Understanding the biological functions and regulatory networks of WDR54 could provide insights into its contributions to disease mechanisms, thereby advancing the development of novel therapeutic strategies against conditions characterized by WDR54 dysregulation. Overall, the study of recombinant WDR54 protein not only enhances our understanding of fundamental cellular processes but may also reveal critical information that could be leveraged in biomedical applications.
