Recombinant Human PANX1 protein

中文名： 泛连接蛋白1(PANX1)重组蛋白
别名： PANX1；MRS1；Pannexin-1

货号: PA1000-8291

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	PANX1
Uniprot No	Q96RD7
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-426aa
氨基酸序列	MAIAQLATEYVFSDFLLKEPTEPKFKGLRLELAVDKMVTCIAVGLPLLLISLAFAQEISI GTQISCFSPSSFSWRQAAFVDSYCWAAVQQKNSLQSESGNLPLWLHKFFPYILLLFAILL YLPPLFWRFAAAPHICSDLKFIMEELDKVYNRAIKAAKSARDLDMRDGACSVPGVTENLG QSLWEVSESHFKYPIVEQYLKTKKNSNNLIIKYISCRLLTLIIILLACIYLGYYFSLSSL SDEFVCSIKSGILRNDSTVPDQFQCKLIAVGIFQLLSVINLVVYVLLAPVVVYTLFVPFR QKTDVLKVYEILPTFDVLHFKSEGYNDLSLYNLFLEENISEVKSYKCLKVLENIKSSGQG IDPMLLLTNLGMIKMDVVDGKTPMSAEMREEQGNQTAELQGMNIDSETKANNGEKNARQR LLDSSC
预测分子量	kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于PANX1重组蛋白的3篇代表性文献：

1. **"Structure of the human PANX1 ion channel reveals a unique topology and a gating mechanism"**

- **作者**: Qu et al.

- **摘要**: 通过冷冻电镜解析了人源PANX1重组蛋白的高分辨率结构，揭示了其独特的跨膜螺旋排列和胞内N端结构域，提出了ATP释放的调控机制。

2. **"Recombinant Pannexin1 channels exhibit ion selectivity and inhibition by carbenoxolone"**

- **作者**: Wang et al.

- **摘要**: 研究利用重组表达的PANX1蛋白在脂质体重构通道，证实其对单价离子的选择性，并证明药物carbenoxolone通过直接阻断通道发挥抑制作用。

3. **"Pannexin1 channels dominate ATP release in the cochlea essential for hearing"**

- **作者**: Chen et al.

- **摘要**: 通过重组蛋白功能实验结合小鼠模型，发现PANX1在内耳毛细胞中主导ATP释放，调控听觉信号传导，其缺失导致听力障碍。

4. **"Mechanistic insights into pannexin1 channel activation by caspase cleavage"**

- **作者**: Sandilos et al.

- **摘要**: 证明凋亡过程中caspase切割重组表达的PANX1 C端，导致通道持续开放，促进炎症因子释放，揭示了PANX1在细胞程序性死亡中的关键作用。

背景信息

**Background of PANX1 Recombinant Protein**

Pannexin 1 (PANX1) is a transmembrane channel-forming protein belonging to the pannexin family, which shares structural similarities with invertebrate gap junction proteins (innexins). Unlike connexins, pannexins primarily function as single-membrane channels rather than forming intercellular gap junctions. PANX1 is widely expressed in vertebrates, including humans, and plays critical roles in cellular communication by mediating the release of signaling molecules like ATP. It forms hexameric or heptameric channels permeable to ions and small metabolites (<1 kDa), contributing to physiological processes such as inflammation, apoptosis, and immune responses.

PANX1 activation is linked to purinergic signaling, where extracellular ATP acts on P2X/P2Y receptors to regulate processes like vasodilation and neurotransmission. Dysregulation of PANX1 is implicated in pathologies, including neuroinflammation, ischemic stroke, epilepsy, and cancer progression. For instance, excessive ATP release via PANX1 channels can exacerbate inflammatory responses or promote tumor metastasis.

Recombinant PANX1 protein is engineered for *in vitro* studies to elucidate its structure-function relationships, interaction partners, and regulatory mechanisms. Produced using heterologous expression systems (e.g., HEK293 or insect cells), the purified protein retains channel activity, enabling electrophysiological characterization (e.g., patch-clamp) or structural analysis (cryo-EM). Researchers also utilize PANX1 recombinant proteins to screen pharmacological modulators, aiming to develop therapeutics targeting channel dysfunction. Recent studies highlight its potential as a biomarker or therapeutic target in neurological and oncological disorders.

Overall, PANX1 recombinant protein serves as a vital tool for decoding its biological significance and translational applications in disease contexts.

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