| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UQCRB |
| Uniprot No | P14927 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-111aa |
| 氨基酸序列 | AGKQAVSAS GKWLDGIRKW YYNAAGFNKL GLMRDDTIYE DEDVKEAIRR LPENLYNDRM FRIKRALDLN LKHQILPKEQ WTKYEEENFY LEPYLKEVIR ERKEREEWAK K |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于UQCRB重组蛋白的3篇文献示例(注：部分内容基于真实研究领域，但文献为模拟示例)：
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1. **文献名称**：*"Recombinant UQCRB Expression and Its Role in Mitochondrial Electron Transport Chain Assembly"*
**作者**：Huttlin EL, et al.
**摘要**：研究通过大肠杆菌系统重组表达UQCRB蛋白，分析其在线粒体复合体III组装中的作用，发现UQCRB缺失导致电子传递链功能异常，影响细胞能量代谢。
2. **文献名称**：*"Structural Insights into UQCRB by Recombinant Protein Crystallography"*
**作者**：Kim JH, et al.
**摘要**：利用重组UQCRB蛋白进行X射线晶体学研究，解析其三维结构，揭示其与泛醌结合的关键位点，为靶向UQCRB的药物设计提供依据。
3. **文献名称**：*"UQCRB Overexpression via Recombinant Technology Enhances Cancer Cell Proliferation"*
**作者**：Smith GP, et al.
**摘要**：通过哺乳动物细胞重组过表达UQCRB，发现其通过调控活性氧(ROS)水平促进肿瘤细胞增殖，提示UQCRB作为潜在癌症治疗靶点。
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**说明**：
- UQCRB(泛醌-细胞色素c还原酶结合蛋白)是线粒体呼吸链复合体III的核心亚基，重组蛋白研究多聚焦于其结构、功能及疾病关联(如癌症、代谢紊乱)。
- 实际研究中可查询**PubMed**或**Web of Science**数据库，关键词如“UQCRB recombinant protein”或“UQCRB expression”。
**Background of UQCRB Recombinant Protein**
UQCRB (Ubiquinol-Cytochrome c Reductase Binding Protein), a subunit of mitochondrial Complex III (cytochrome *bc*₁ complex), plays a critical role in the electron transport chain (ETC) by facilitating electron transfer from ubiquinol to cytochrome *c* and contributing to proton gradient generation for ATP synthesis. Encoded by the nuclear *UQCRB* gene, this 13.4 kDa protein anchors the Rieske iron-sulfur protein within Complex III, ensuring structural stability and functional efficiency of the ETC.
Recombinant UQCRB protein is engineered via heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce purified, biologically active forms for research. Its recombinant form retains the ability to interact with Complex III components, making it valuable for studying mitochondrial dysfunction, oxidative phosphorylation, and diseases linked to ETC defects, such as neurodegenerative disorders, metabolic syndromes, and cancers.
Notably, UQCRB has gained attention for its "moonlighting" roles beyond the ETC. It interacts with the hepatitis B virus X protein (HBx), promoting viral replication, and acts as a potential oncoprotein in gliomas and lung cancers by regulating hypoxia-inducible factor 1α (HIF-1α) and reactive oxygen species (ROS) signaling. These non-canonical functions highlight its therapeutic potential. Recombinant UQCRB enables high-throughput screening for inhibitors targeting its pathological interactions, aiding drug development.
Research also leverages recombinant UQCRB to explore its structural dynamics, post-translational modifications, and role in apoptosis, offering insights into mitochondrial diseases and aging. Overall, it serves as a versatile tool for both basic and translational studies in bioenergetics and precision medicine.
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