| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PTBP1 |
| Uniprot No | P26599-3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-557aa |
| 氨基酸序列 | MDGIVPDIAVGTKRGSDELFSTCVTNGPFIMSSNSASAANGNDSKKFKGD SRSAGVPSRVIHIRKLPIDVTEGEVISLGLPFGKVTNLLMLKGKNQAFIE MNTEEAANTMVNYYTSVTPVLRGQPIYIQFSNHKELKTDSSPNQARAQAA LQAVNSVQSGNLALAASAAAVDAGMAMAGQSPVLRIIVENLFYPVTLDVL HQIFSKFGTVLKIITFTKNNQFQALLQYADPVSAQHAKLSLDGQNIYNAC CTLRIDFSKLTSLNVKYNNDKSRDYTRPDLPSGDSQPSLDQTMAAAFGAP GIISASPYAGAGFPPTFAIPQAAGLSVPNVHGALAPLAIPSAAAAAAAAG RIAIPGLAGAGNSVLLVSNLNPERVTPQSLFILFGVYGDVQRVKILFNKK ENALVQMADGNQAQLAMSHLNGHKLHGKPIRITLSKHQNVQLPREGQEDQ GLTKDYGNSPLHRFKKPGSKNFQNIFPPSATLHLSNIPPSVSEEDLKVLF SSNGGVVKGFKFFQKDRKMALIQMGSVEEAVQALIDLHNHDLGENHHLRV SFSKSTI |
| 预测分子量 | 87 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PTBP1重组蛋白的3篇参考文献及其摘要概括：
---
1. **文献名称**: *Structure of PTBP1 RRM3 bound to RNA reveals a novel mode of tandem domain recognition*
**作者**: Oberstrass FC et al.
**摘要**: 该研究通过X射线晶体学解析了PTBP1重组蛋白的第三个RNA识别结构域(RRM3)与RNA复合物的结构，揭示了PTBP1通过串联RRM结构域结合RNA的独特模式，并阐明了其选择性识别多聚嘧啶序列的分子机制。
---
2. **文献名称**: *PTBP1 promotes tumorigenesis by regulating apoptosis and cell cycle via alternative splicing*
**作者**: He X et al.
**摘要**: 研究利用重组PTBP1蛋白进行体外功能实验，发现其通过调控Bcl-x和Cyclin D1等基因的选择性剪接，影响肿瘤细胞凋亡和周期进程，揭示了PTBP1在癌症发生中的关键作用。
---
3. **文献名称**: *A switch in RBP binding affinity regulates alternative splicing through competition with a miRNA*
**作者**: Makeyev EV et al.
**摘要**: 该研究通过重组PTBP1蛋白结合实验，证明PTBP1与microRNA在靶RNA位点的竞争性结合可动态调控神经元分化相关基因(如Nova1)的剪接模式，揭示了RNA结合蛋白与miRNA的互作网络。
---
**备注**：以上文献摘要为简化概括，实际研究可能涉及更复杂的实验设计。如需具体文献，建议通过PubMed或期刊数据库检索标题或作者名获取全文。
PTBP1 (Polypyrimidine Tract-Binding Protein 1), also known as PTB or hnRNP I, is a ubiquitously expressed RNA-binding protein that plays critical roles in post-transcriptional gene regulation. It contains four RNA recognition motifs (RRMs) that enable interactions with pyrimidine-rich sequences in pre-mRNAs and other RNAs. PTBP1 is involved in alternative splicing, mRNA stability, localization, and translation, often acting as a repressor or activator depending on cellular context. For example, it regulates splicing events in genes related to cell proliferation, apoptosis, and differentiation. Dysregulation of PTBP1 is linked to cancers, neurological disorders, and viral infections, where it may influence oncogenic splicing variants or viral RNA processing.
Recombinant PTBP1 protein is engineered for in vitro studies to dissect its molecular mechanisms. Typically produced in *E. coli* or mammalian expression systems, it retains RNA-binding activity and is used in assays like electrophoretic mobility shift assays (EMSAs), pull-down experiments, or structural studies. Researchers employ it to map RNA-protein interactions, study spliceosome assembly, or screen for inhibitors targeting its oncogenic functions. In cellular reprogramming, PTBP1 has gained attention for its role in converting astrocytes to neurons, highlighting its therapeutic potential. The recombinant form allows standardized, controlled experimentation, bypassing challenges of endogenous protein purification. Its availability accelerates research into RNA biology, disease pathways, and therapeutic development, making it a vital tool for understanding PTBP1's multifunctional landscape.
×
