关键信息
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基因名
ChAT
- 应用
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别名
ChAT；Choline O-acetyltransferase
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P28329
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表达区间
120-733aa
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氨基酸序列
AAKTPSSEESGLPKLPVPPLQQTLATYLQCMRHLVSEEQFRKSQAIVQQFGAPGGLGETLQQKLLERQEKTANWVSEYWLNDMYLNNRLALPVNSSPAVIFARQHFPGTDDQLRFAASLISGVLSYKALLDSHSIPTDCAKGQLSGQPLCMKQYYGLFSSYRLPGHTQDTLVAQNSSIMPEPEHVIVACCNQFFVLDVVINFRRLSEGDLFTQLRKIVKMASNEDERLPPIGLLTSDGRSEWAEARTVLVKDSTNRDSLDMIERCICLVCLDAPGGVELSDTHRALQLLHGGGYSKNGANRWYDKSLQFVVGRDGTCGVVCEHSPFDGIVLVQCTEHLLKHVTQSSRKLIRADSVSELPAPRRLRWKCSPEIQGHLASSAEKLQRIVKNLDFIVYKFDNYGKTFIKKQKCSPDAFIQVALQLAFYRLHRRLVPTYESASIRRFQEGRVDNIRSATPEALAFVRAVTDHKAAVPASEKLLLLKDAIRAQTAYTVMAITGMAIDNHLLALRELARAMCKELPEMFMDETYLMSNRFVLSTSQVPTTTEMFCCYGPVVPNGYGACYNPQPETILFCISSFHSCKETSSSKFAKAVEESLIDMRDLCSLLPPTESKPL
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分子量
76.1 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
ChAT, or choline acetyltransferase, is a crucial enzyme involved in the biosynthesis of acetylcholine, a key neurotransmitter that plays a vital role in various physiological processes, including muscle activation and cognitive function. Research into ChAT has gained significant attention due to its implications in neurodegenerative diseases, particularly in conditions such as Alzheimer's disease, where reduced levels of acetylcholine are observed. Understanding the structure and function of ChAT can lead to better insights into the mechanisms underlying these diseases and potentially aid in the development of targeted therapies. Recombinant ChAT proteins are essential for this research as they enable detailed studies of the enzyme's biochemical properties, interactions, and potential as a therapeutic target. By utilizing advanced protein engineering techniques, scientists can produce ChAT with specific modifications, offering a platform for discovering new drugs that could enhance cholinergic signaling in the brain. Furthermore, investigating ChAT through recombinant approaches can help elucidate its role in synaptic transmission and plasticity, thereby advancing our understanding of neurological disorders associated with acetylcholine dysfunction. Overall, the study of recombinant ChAT proteins is a promising avenue for unraveling the complexities of cholinergic signaling and its implications in health and disease.
