| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PMFBP1 |
| Uniprot No | Q8TBY8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1007aa |
| 氨基酸序列 | MKDEAGERDREVSSLNSKLLSLQLDIKNLHDVCKRQRKTLQDNQLCMEEAMNSSHDKKQAQALAFEESEVEFGSSKQCHLRQLQQLKKKLLVLQQELEFHTEELQTSYYSLRQYQSILEKQTSDLVLLHHHCKLKEDEVILYEEEMGNHNENTGEKLHLAQEQLALAGDKIASLERSLNLYRDKYQSSLSNIELLECQVKMLQGELGGIMGQEPENKGDHSKVRIYTSPCMIQEHQETQKRLSEVWQKVSQQDDLIQELRNKLACSNALVLEREKALIKLQADFASCTATHRYPPSSSEECEDIKKILKHLQEQKDSQCLHVEEYQNLVKDLRVELEAVSEQKRNIMKDMMKLELDLHGLREETSAHIERKDKDITILQCRLQELQLEFTETQKLTLKKDKFLQEKDEMLQELEKKLTQVQNSLLKKEKELEKQQCMATELEMTVKEAKQDKSKEAECKALQAEVQKLKNSLEEAKQQERLAAQQAAQCKEEAALAGCHLEDTQRKLQKGLLLDKQKADTIQELQRELQMLQKESSMAEKEQTSNRKRVEELSLELSEALRKLENSDKEKRQLQKTVAEQDMKMNDMLDRIKHQHREQGSIKCKLEEDLQEATKLLEDKREQLKKSKEHEKLMEGELEALRQEFKKKDKTLKENSRKLEEENENLRAELQCCSTQLESSLNKYNTSQQVIQDLNKEIALQKESLMSLQAQLDKALQKEKHYLQTTITKEAYDALSRKSAACQDDLTQALEKLNHVTSETKSLQQSLTQTQEKKAQLEEEIIAYEERMKKLNTELRKLRGFHQESELEVHAFDKKLEEMSCQVLQWQKQHQNDLKMLAAKEEQLREFQEEMAALKENLLEDDKEPCCLPQWSVPKDTCRLYRGNDQIMTNLEQWAKQQKVANEKLGNQLREQVKYIAKLSGEKDHLHSVMVHLQQENKKLKKEIEEKKMKAENTRLCTKALGPSRTESTQREKVCGTLGWKGLPQDMGQRMDLTKYIGMPHCPGSSYC |
| 预测分子量 | 117,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与PMFBP1重组蛋白相关的参考文献(注：PMFBP1相关研究较少，以下为模拟示例)：
1. **文献名称**：PMFBP1 is essential for sperm acrosome formation and male fertility in mice
**作者**：Zhou et al. (2020)
**摘要**：研究通过重组PMFBP1蛋白体外功能实验，证实其参与小鼠精子顶体形成的分子机制，并发现PMFBP1缺失导致雄性不育。
2. **文献名称**：Recombinant PMFBP1 purification and interaction with spermatogenesis-related proteins
**作者**：Wang et al. (2019)
**摘要**：报道了利用大肠杆菌系统高效表达PMFBP1重组蛋白的纯化方法，并通过Pull-down实验揭示其与SPATA16等精子形成相关蛋白的相互作用。
3. **文献名称**：PMFBP1 mutations disrupt histone-to-protamine transition in human spermatogenesis
**作者**：Kherraf et al. (2018)
**摘要**：通过重组PMFBP1蛋白的体外染色质重塑实验，证明其在组蛋白-鱼精蛋白替换过程中的关键作用，突变可导致精子DNA异常压缩。
注：实际文献需通过PubMed或Web of Science以"PMFBP1 recombinant protein"等关键词检索，部分研究可能侧重于基因功能而非重组蛋白应用。建议结合具体研究方向补充检索策略。
PMFBP1 (Polyamine Modulated Factor Binding Protein 1) is a protein-coding gene implicated in cellular processes such as spermatogenesis, cell cycle regulation, and polyamine metabolism. The gene, located on human chromosome 16q23.1. encodes a 684-amino-acid protein containing conserved phosphorylation sites and potential nuclear localization signals. PMFBP1 was initially identified through its interaction with polyamines, organic cations critical for DNA stability and cell proliferation, suggesting a role in modulating polyamine-dependent pathways.
Functionally, PMFBP1 is essential for male fertility. Studies link its deficiency to teratozoospermia, a condition characterized by abnormal sperm morphology and male infertility. PMFBP1 interacts with proteins involved in sperm head shaping (e.g., SPATA16. HSPA4) during spermiogenesis, and its absence disrupts cytoskeletal dynamics, leading to malformed sperm heads and flagella. Additionally, PMFBP1 may act as a tumor suppressor in certain cancers, with downregulation observed in hepatocellular carcinoma and breast cancer, though its oncogenic mechanisms remain under investigation.
Recombinant PMFBP1 protein is typically produced in Escherichia coli or mammalian expression systems, often fused with tags (e.g., His, GST) for purification. It serves as a critical reagent for functional studies, antibody generation, and diagnostic assays. Researchers utilize it to explore protein-protein interactions, post-translational modifications, and structural features via techniques like pull-down assays, crystallography, or ELISA. Clinically, recombinant PMFBP1 aids in developing fertility diagnostics and understanding mutation-associated infertility. Ongoing research aims to elucidate its dual roles in reproduction and cancer, positioning it as a potential therapeutic target or biomarker.
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