关键信息
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基因名
UGT3A2
- 应用
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别名
EC 2.4.1.17; MGC119426; MGC119429; OTTHUMP00000221219; UD3A2_HUMAN; UDP glucuronosyltransferase 3A2; UDP glycosyltransferase 3 family polypeptide A2; UDP-glucuronosyltransferase 3A2; UDPGT 3A2; Ugt3a2
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q3SY77
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表达区间
1-523 aa
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氨基酸序列
MAGQRVLLLVGFLLPGVLLSEAAKILTISTVGGSHYLLMDRVSQILQDHGHNVTMLNHKRGPFMPDFKKEEKSYQVISWLAPEDHQREFKKSFDFFLEETLGGRGKFENLLNVLEYLALQCSHFLNRKDIMDSLKNENFDMVIVETFDYCPFLIAEKLGKPFVAILSTSFGSLEFGLPIPLSYVPVFRSLLTDHMDFWGRVKNFLMFFSFCRRQQHMQSTFDNTIKEHFTEGSRPVLSHLLLKAELWFINSDFAFDFARPLLPNTVYVGGLMEKPIKPVPQDLENFIAKFGDSGFVLVTLGSMVNTCQNPEIFKEMNNAFAHLPQGVIWKCQCSHWPKDVHLAANVKIVDWLPQSDLLAHPSIRLFVTHGGQNSIMEAIQHGVPMVGIPLFGDQPENMVRVEAKKFGVSIQLKKLKAETLALKMKQIMEDKRYKSAAVAASVILRSHPLSPTQRLVGWIDHVLQTGGATHLKPYVFQQPWHEQYLLDVFVFLLGLTLGTLWLCGKLLGMAVWWLRGARKVKET
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分子量
85.9 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
UGT3A2, a member of the UDP-glucuronosyltransferase (UGT) family, plays a crucial role in the metabolism of various endogenous and exogenous compounds by catalyzing the glucuronidation process. This enzymatic activity is vital for detoxifying substances, enhancing their solubility, and facilitating their excretion from the body. Research on UGT3A2 has gained importance due to its involvement in drug metabolism, particularly in the context of pharmacogenomics, where genetic variations can influence individual responses to medications. Understanding UGT3A2's structure, function, and polymorphisms is essential for predicting drug efficacy and safety, as certain mutations may lead to altered metabolism and increased risk of adverse drug reactions. Furthermore, UGT3A2 is also implicated in the metabolism of several carcinogens and hormones, leading to growing interest in its potential role in cancer biology and endocrine regulation. The recombinant expression of UGT3A2 allows for detailed studies of its enzymatic kinetics, substrate specificity, and interactions with inhibitors, facilitating a deeper understanding of its biological significance and therapeutic potential. Overall, the study of UGT3A2 is not only pivotal for advancing pharmacotherapy but also for developing personalized medicine approaches that optimize treatment outcomes for diverse patient populations.
