| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GR |
| Uniprot No | P00390 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-777aa |
| 氨基酸序列 | MDSKESLTPGREENPSSVLAQERGDVMDFYKTLRGGATVKVSASSPSLAV ASQSDSKQRRLLVDFPKGSVSNAQQPDLSKAVSLSMGLYMGETETKVMGN DLGFPQQGQISLSSGETDLKLLEESIANLNRSTSVPENPKSSASTAVSAA PTEKEFPKTHSDVSSEQQHLKGQTGTNGGNVKLYTTDQSTFDILQDLEFS SGSPGKETNESPWRSDLLIDENCLLSPLAGEDDSFLLEGNSNEDCKPLIL PDTKPKIKDNGDLVLSSPSNVTLPQVKTEKEDFIELCTPGVIKQEKLGTV YCQASFPGANIIGNKMSAISVHGVSTSGGQMYHYDMNTASLSQQQDQKPI FNVIPPIPVGSENWNRCQGSGDDNLTSLGTLNFPGRTVFSNGYSSPSMRP DVSSPPSSSSTATTGPPPKLCLVCSDEASGCHYGVLTCGSCKVFFKRAVE GQHNYLCAGRNDCIIDKIRRKNCPACRYRKCLQAGMNLEARKTKKKIKGI QQATTGVSQETSENPGNKTIVPATLPQLTPTLVSLLEVIEPEVLYAGYDS SVPDSTWRIMTTLNMLGGRQVIAAVKWAKAIPGFRNLHLDDQMTLLQYSW MFLMAFALGWRSYRQSSANLLCFAPDLIINEQRMTLPCMYDQCKHMLYVS SELHRLQVSYEEYLCMKTLLLLSSVPKDGLKSQELFDEIRMTYIKELGKA IVKREGNSSQNWQRFYQLTKLLDSMHEVVENLLNYCFQTFLDKTMSIEFP EMLAEIITNQIPKYSNGNIKKLLFHQK |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于糖皮质激素受体(Glucocorticoid Receptor, GR)重组蛋白的参考文献及摘要概括：
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1. **文献名称**：*Crystallographic analysis of the interaction of the glucocorticoid receptor with DNA*
**作者**：Luisi, B.F. et al.
**摘要**：该研究通过X射线晶体学解析了糖皮质激素受体DNA结合域(DBD)与DNA的复合物结构，揭示了受体通过锌指结构识别特定DNA序列的分子机制，为理解GR的转录调控功能提供了结构基础。
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2. **文献名称**：*Expression and purification of functional recombinant glucocorticoid receptor from Escherichia coli*
**作者**：Hollenberg, S.M., Evans, R.M.
**摘要**：报道了一种利用大肠杆菌表达系统高效表达重组GR蛋白的方法，并通过体外实验验证了重组蛋白的激素结合活性和DNA结合能力，为大规模制备功能性GR用于生化研究奠定基础。
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3. **文献名称**：*Structural basis for nuclear hormone receptor signaling via glucocorticoid receptor*
**作者**：Bledsoe, R.K. et al.
**摘要**：通过解析GR配体结合域(LBD)与激动剂/拮抗剂的复合物结构，阐明了激素结合如何诱导受体构象变化并影响共调节蛋白的招募，为设计选择性GR调节剂(SEGRMs)提供了理论依据。
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4. **文献名称**：*Mechanisms of glucocorticoid receptor signaling during inflammation*
**作者**：Oakley, R.H., Cidlowski, J.A.
**摘要**：综述了GR重组蛋白在炎症反应中的信号转导机制，包括其与热休克蛋白的相互作用、核易位过程及靶基因调控，强调了重组GR模型在药物抗炎研究中的应用价值。
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这些文献涵盖了GR重组蛋白的结构解析、表达策略、功能机制及转化研究，可作为相关领域的核心参考资料。
**Background of GR Recombinant Proteins**
Glucocorticoid receptor (GR), a member of the nuclear receptor superfamily, plays a pivotal role in mediating the effects of glucocorticoids, which regulate diverse physiological processes such as stress response, immune function, metabolism, and development. GR functions as a ligand-dependent transcription factor, translocating to the nucleus upon binding cortisol (or synthetic analogs like dexamethasone) to modulate gene expression. Its structure includes an N-terminal transcriptional activation domain, a central DNA-binding domain, and a C-terminal ligand-binding domain.
Recombinant GR proteins are engineered using genetic cloning techniques, where the GR gene is inserted into expression vectors and produced in host systems like *E. coli*, yeast, or mammalian cells*. These systems enable large-scale production of functional GR, often tagged with markers (e.g., GST, His-tag) for purification and detection. Recombinant GR retains ligand-binding and DNA-binding activities, making it invaluable for *in vitro* studies, including ligand screening, mutational analysis, and interaction assays with co-regulators or DNA response elements.
The development of GR recombinant proteins has advanced research into glucocorticoid signaling dysregulation linked to diseases such as inflammation, autoimmune disorders, and cancer. It also facilitates structural studies (e.g., X-ray crystallography, cryo-EM) to resolve GR-ligand or GR-DNA complexes, guiding the design of selective glucocorticoid receptor modulators (SEGRMs) with reduced side effects. Additionally, recombinant GR is used in high-throughput drug discovery to identify novel therapeutics targeting GR pathways.
Overall, GR recombinant proteins serve as essential tools for deciphering glucocorticoid biology and developing precision therapies, bridging molecular insights with clinical applications.
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