| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NFIB |
| Uniprot No | O00712 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-420aa |
| 氨基酸序列 | MYSPICLTQ DEFHPFIEAL LPHVRAIAYT WFNLQARKRK YFKKHEKRMS KDEERAVKDE LLSEKPEIKQ KWASRLLAKL RKDIRQEYRE DFVLTVTGKK HPCCVLSNPD QKGKIRRIDC LRQADKVWRL DLVMVILFKG IPLESTDGER LMKSPHCTNP ALCVQPHHIT VSVKELDLFL AYYVQEQDSG QSGSPSHNDP AKNPPGYLED SFVKSGVFNV SELVRVSRTP ITQGTGVNFP IGEIPSQPYY HDMNSGVNLQ RSLSSPPSSK RPKTISIDEN MEPSPTGDFY PSPSSPAAGS RTWHERDQDM SSPTTMKKPE KPLFSSASPQ DSSPRLSTFP QHHHPGIPGV AHSVISTRTP PPPSPLPFPT QAILPPAPSS YFSHPTIRYP PHLNPQDTLK NYVPSYDPSS PQTSQSWYLG |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NFIB重组蛋白的示例参考文献(注：文献信息为示例性概括，实际文献需通过学术数据库检索)：
1. **文献名称**: "Functional characterization of recombinant NFIB in lung development"
**作者**: Smith J, et al.
**摘要**: 研究通过大肠杆菌系统表达重组NFIB蛋白，并验证其在小鼠肺上皮细胞分化中的调控作用，揭示NFIB通过特定信号通路影响肺泡形成。
2. **文献名称**: "NFIB recombinant protein promotes neuronal differentiation in vitro"
**作者**: Lee H, et al.
**摘要**: 利用昆虫细胞表达系统制备高纯度NFIB重组蛋白，实验证明其能激活神经干细胞中分化相关基因，为神经再生研究提供工具。
3. **文献名称**: "Structural analysis of NFIB-DNA binding domain using recombinant protein"
**作者**: Garcia R, et al.
**摘要**: 通过X射线晶体学解析重组NFIB蛋白的DNA结合域结构，阐明其与靶基因启动子结合的关键氨基酸位点及作用机制。
4. **文献名称**: "Recombinant NFIB as a biomarker in cancer progression"
**作者**: Wang Q, et al.
**摘要**: 研究开发基于重组NFIB蛋白的检测方法，发现其在肺癌患者血清中的异常表达与肿瘤转移相关性，提示其潜在临床诊断价值。
建议通过PubMed、Web of Science等平台以关键词"NFIB recombinant protein"或"NFIB purification"检索真实文献，重点关注蛋白功能、制备方法或疾病机制研究。
The nuclear factor I B (NFIB) is a member of the Nuclear Factor I (NFI) family of transcription factors, which play critical roles in DNA replication, chromatin remodeling, and transcriptional regulation. NFIB specifically binds to palindromic DNA sequences to either activate or repress gene expression, depending on cellular context. It is involved in diverse biological processes, including organ development (e.g., lung, brain, and liver), cell differentiation, and tumorigenesis. Dysregulation of NFIB has been linked to cancers such as small cell lung cancer, where genomic amplification or chromosomal rearrangements drive its oncogenic activity, and in breast cancer, where it modulates metastasis.
Recombinant NFIB protein is engineered using expression systems like *E. coli* or mammalian cells to produce purified, functional NFIB for research applications. This protein typically retains key domains, including the N-terminal DNA-binding domain and C-terminal transactivation domain, enabling studies on its interactions with DNA, co-regulatory proteins, or signaling pathways. Recombinant NFIB is often tagged (e.g., His, GST, or FLAG tags) for ease of purification and detection. Researchers use it to investigate NFIB's role in gene regulatory networks, its post-translational modifications (e.g., phosphorylation), or its crosstalk with pathways like Wnt or Notch. In disease models, it serves as a tool to explore therapeutic targeting of NFIB-driven processes, such as cancer metastasis or developmental disorders. Its applications span *in vitro* assays (EMSA, ChIP), cell-based studies, and *in vivo* functional validations.
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