| 纯度 | >90%SDS-PAGE. |
| 种属 | mouse |
| 靶点 | LMNB2 |
| Uniprot No | P21619 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-593aa |
| 氨基酸序列 | MASLPPHAGP ATPLSPTRLS RLQEKEELRE LNDRLAHYID RVRALELEND RLLLRISEKE EVTTREVSGI KTLYESELAD ARRVLDETAR ERARLQIEIG KVQAELEEAR KSAKKREGEL TVAQGRVKDL ESLFHRSEAE LATALSDKQG LETEVAELRA QLAKAEDGHA VAKKQLEKET LMRVDLENRC QSLQEELAFS KSVFEEEVRE TRRRHERRLV EVDSSRQQEY DFKMAQALED LRSQHDEQVR LYRVELEQTY QAKLDNAKLL SDQNDKAAHA AREELKEARM RVESLSYQLL GLQKQASAAE NHIHELEEAL AGERDKFRKM LDAKEQEMTE VRDAMQQQLA EYQELLDIKL ALDMEISAYR KLLEGEEERL KLSPSPSSRI TISRATSSSS SSSGVGMSVG QGRGKRRRLE TEDTSGSPSR ASRVSSGSRL AQQTVATGVV NIDEVDPEGR FVRLKNSSDK DQSLGNWRIK RQVLEGEDIA YKFTPKYVLR AGQTVTVWAA GAGATHSPPS TLVWKSQTNW GPGESFRTAL VSADGEEVAV KAAKHSSVQG RENGEEEEEE EAEFGEEDLF HQQGDPRTTS RGC |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LMNB2重组蛋白的3篇代表性文献(注：示例基于假设性研究，建议通过学术数据库验证最新成果)：
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1. **文献名称**：*"Recombinant LMNB2 facilitates nuclear envelope assembly in vitro"*
**作者**：Someya, K. et al.
**摘要**：研究通过在大肠杆菌中表达重组人源LMNB2蛋白，发现其在体外核膜重建模型中能促进膜泡融合及核孔复合体招募，揭示了LMNB2在核结构组装中的关键作用。
2. **文献名称**：*"Phosphorylation of recombinant lamin B2 regulates mitotic progression"*
**作者**：Zhang, L. et al.
**摘要**：利用昆虫细胞系统表达重组LMNB2.证实其特定位点磷酸化调控有丝分裂中核纤层解聚，缺失磷酸化修饰会导致染色体分离异常，提示其在细胞周期中的功能机制。
3. **文献名称**：*"Structural insights into LMNB2-DNA interactions using recombinant protein"*
**作者**：Thompson, R. et al.
**摘要**：通过纯化重组LMNB2并进行X射线晶体学分析，鉴定了其与染色质结合的C端结构域，为理解核纤层蛋白维持基因组空间组织的分子基础提供了新证据。
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**建议**：可通过PubMed或Google Scholar搜索关键词“LMNB2 recombinant protein”、“lamin B2 expression”或结合具体研究领域(如“癌症”“核结构”)获取最新文献。部分真实相关研究可能涉及LMNB2在疾病模型(如早衰症、癌症转移)中的重组表达与功能验证。
**Background of LMNB2 Recombinant Protein**
LMNB2 (Lamin B2) is a member of the lamin family of nuclear intermediate filament proteins, essential for maintaining nuclear structure, chromatin organization, and mechanical stability of the nucleus. As a key component of the nuclear lamina, LMNB2 interacts with other lamins, chromatin-binding proteins, and nuclear envelope components to regulate processes like DNA replication, cell cycle progression, and gene expression. Unlike its somatic counterparts (e.g., Lamin A/C), LMNB2 is ubiquitously expressed and plays distinct roles in mitotic spindle formation, nuclear envelope reassembly, and cellular differentiation.
Recombinant LMNB2 protein is produced *in vitro* using heterologous expression systems (e.g., *E. coli* or mammalian cells*) to enable functional studies. Its recombinant form typically retains post-translational modifications (e.g., phosphorylation) critical for its dynamic behavior during mitosis. Researchers leverage LMNB2 recombinant protein to investigate nuclear architecture defects linked to diseases, including laminopathies (e.g., progeria), cancer (aberrant LMNB2 expression correlates with metastasis), and age-related disorders. It is also used to study mechanisms of cellular senescence, viral infection (viruses often target lamins for nuclear entry), and drug screening for lamin-related pathologies.
The protein’s high purity and activity in *in vitro* assays (e.g., binding studies, enzymatic activity modulation) make it a valuable tool for dissecting lamin interactions and nuclear mechanics. Its role in mitotic regulation further underscores its relevance in developmental biology and regenerative medicine.
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