关键信息
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基因名
GJB3
- 应用
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别名
GJB3; CX31; Gap junction beta-3 protein; Connexin-31; Cx31
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O75712
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表达区间
1-270aa
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氨基酸序列
MDWKTLQALLSGVNKYSTAFGRIWLSVVFVFRVLVYVVAAERVWGDEQKDFDCNTKQPGCTNVCYDNYFPISNIRLWALQLIFVTCPSLLVILHVAYREERERRHRQKHGDQCAKLYDNAGKKHGGLWWTYLFSLIFKLIIEFLFLYLLHTLWHGFNMPRLVQCANVAPCPNIVDCYIARPTEKKIFTYFMVGASAVCIVLTICELCYLICHRVLRGLHKDKPRGGCSPSSSASRASTCRCHHKLVEAGEVDPDPGNNKLQASAPNLTPI
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分子量
55.44 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
GJB3, also known as connexin 31, is a member of the connexin family of proteins that form gap junctions, allowing for direct intercellular communication. Mutations in the GJB3 gene are linked to various genetic disorders, primarily non-syndromic hearing loss (NSHL), particularly in certain populations. Understanding the structure and function of GJB3 is crucial for elucidating the molecular mechanisms underlying these diseases, as gap junctions play essential roles in maintaining cellular homeostasis and facilitating communication between adjacent cells. Recent advancements in recombinant protein technology have enabled researchers to produce and study GJB3 in vitro, providing insights into its functional properties and the impact of specific mutations on protein behavior. This research not only enhances our understanding of the pathophysiology associated with GJB3 mutations but also serves as a foundation for exploring potential therapeutic strategies for hearing loss and other related conditions. Investigating GJB3’s role in cellular pathways and its interactions with other proteins could lead to novel approaches in treating auditory and other connexin-related disorders. Overall, the study of GJB3 recombinant protein is pivotal in bridging the gap between genetic mutations and the phenotypic manifestations of associated diseases, paving the way for advancements in molecular medicine and targeted treatments.
