| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BRD8 |
| Uniprot No | Q9H0E9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1235aa |
| 氨基酸序列 | MATGTGKHKLLSTGPTEPWSIREKLCLASSVMRSGDQNWVSVSRAIKPFAEPGRPPDWFSQKHCASQYSELLETTETPKRKRGEKGEVVETVEDVIVRKLTAERVEELKKVIKETQERYRRLKRDAELIQAGHMDSRLDELCNDIATKKKLEEEEAEVKRKATDAAYQARQAVKTPPRRLPTVMVRSPIDSASPGGDYPLGDLTPTTMEEATSGVNESEMAVASGHLNSTGVLLEVGGVLPMIHGGEIQQTPNTVAASPAASGAPTLSRLLEAGPTQFTTPLASFTTVASEPPVKLVPPPVESVSQATIVMMPALPAPSSAPAVSTTESVAPVSQPDNCVPMEAVGDPHTVTVSMDSSEISMIINSIKEECFRSGVAEAPVGSKAPSIDGKEELDLAEKMDIAVSYTGEELDFETVGDIIAIIEDKVDDHPEVLDVAAVEAALSFCEENDDPQSLPGPWEHPIQQERDKPVPLPAPEMTVKQERLDFEETENKGIHELVDIREPSAEIKVEPAEPEPVISGAEIVAGVVPATSMEPPELRSQDLDEELGSTAAGEIVEADVAIGKGDETPLTNVKTEASPESMLSPSHGSNPIEDPLEAETQHKFEMSDSLKEESGTIFGSQIKDAPGEDEEEDGVSEAASLEEPKEEDQGEGYLSEMDNEPPVSESDDGFSIHNATLQSHTLADSIPSSPASSQFSVCSEDQEAIQAQKIWKKAIMLVWRAAANHRYANVFLQPVTDDIAPGYHSIVQRPMDLSTIKKNIENGLIRSTAEFQRDIMLMFQNAVMYNSSDHDVYHMAVEMQRDVLEQIQQFLATQLIMQTSESGISAKSLRGRDSTRKQDASEKDSVPMGSPAFLLSLFMGHEWVWLDSEQDHPNDSELSNDCRSLFSSWDSSLDLDVGNWRETEDPEAEELEESSPEREPSELLVGDGGSEESQEAARKASHQNLLHFLSEVAYLMEPLCISSNESSEGCCPPSGTRQEGREIKASEGERELCRETEELSAKGDPLVAEKPLGENGKPEVASAPSVICTVQGLLTESEEGEAQQESKGEDQGEVYVSEMEDQPPSGECDDAFNIKETPLVDTLFSHATSSKLTDLSQDDPVQDHLLFKKTLLPVWKMIASHRFSSPFLKPVSERQAPGYKDVVKRPMDLTSLKRNLSKGRIRTMAQFLRDLMLMFQNAVMYNDSDHHVYHMAVEMRQEVLEQIQVLNIWLDKRKGSSSLEGEPANPVDDGKPVF |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BRD8重组蛋白的3篇参考文献摘要(基于公开研究内容概括，具体文献需核实)：
1. **文献名称**：*Structural and functional characterization of the recombinant BRD8 bromodomain*
**作者**：Smith A, et al.
**摘要**：该研究利用大肠杆菌表达系统成功制备了重组BRD8溴结构域蛋白，并通过X射线晶体学解析其三维结构，揭示了其与乙酰化组蛋白结合的关键氨基酸位点，为靶向药物设计提供基础。
2. **文献名称**：*BRD8 regulates transcriptional networks in cancer via recombinant protein-mediated chromatin binding*
**作者**：Li X, et al.
**摘要**：作者通过哺乳动物细胞表达纯化BRD8重组蛋白，结合ChIP-seq技术证明其在卵巢癌细胞中通过结合特定乙酰化修饰的染色质区域调控致癌基因表达，提示其作为治疗靶点的潜力。
3. **文献名称**：*Development of a BRD8 recombinant protein-based assay for high-throughput inhibitor screening*
**作者**：Zhang Y, et al.
**摘要**：研究团队优化了BRD8重组蛋白的体外表达与纯化流程，并基于该蛋白建立了一种荧光偏振检测方法，用于高通量筛选靶向BRD8溴结构域的小分子抑制剂，发现多个先导化合物。
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**注**：以上为模拟概括性内容，实际文献需通过PubMed、Google Scholar等平台以“BRD8 recombinant protein”“BRD8 bromodomain”等关键词检索获取。
Bromodomain-containing protein 8 (BRD8) is a member of the bromodomain and extraterminal (BET) family, which includes structurally conserved epigenetic readers that bind acetylated lysine residues on histones and other proteins. BRD8 contains two tandem bromodomains that enable its interaction with acetylated chromatin, playing a role in transcriptional regulation and chromatin remodeling. Unlike other BET proteins (e.g., BRD2. BRD3. BRD4), BRD8 is less characterized but has garnered interest due to its unique functional associations and potential therapeutic implications.
Recombinant BRD8 protein is engineered in vitro using expression systems like *E. coli* or mammalian cells, enabling researchers to study its biochemical properties, structure, and interactions. Studies suggest BRD8 participates in the SWI/SNF chromatin-remodeling complex, influencing gene expression by modulating chromatin accessibility. It also interacts with the tumor suppressor p53. regulating its stability and activity, which links BRD8 to cancer pathways. Dysregulation of BRD8 has been implicated in various cancers, including glioblastoma and ovarian cancer, where it may promote cell proliferation or chemoresistance.
The development of recombinant BRD8 facilitates drug discovery, particularly for BET inhibitors targeting bromodomain-acetyl-lysine interactions. While most BET inhibitors focus on BRD4. BRD8-specific probes could reveal its distinct biological roles. Structural studies using recombinant BRD8 have mapped critical residues for acetyl-lysine binding, aiding in designing selective inhibitors. Additionally, BRD8's role in viral replication (e.g., HIV) and cellular stress responses highlights its broader relevance in disease mechanisms.
Despite progress, BRD8's full functional spectrum remains elusive. Recombinant protein tools are essential for unraveling its interactions with histone variants, non-histone partners, and regulatory networks, offering insights into epigenetic therapies and precision oncology.
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