Recombinant Human BRD8 protein

中文名： 含溴区蛋白8(BRD8)重组蛋白
别名： BRD8；SMAP；SMAP2；Bromodomain-containing protein 8

货号: PA1000-8551

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	BRD8
Uniprot No	Q9H0E9
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-1235aa
氨基酸序列	MATGTGKHKLLSTGPTEPWSIREKLCLASSVMRSGDQNWVSVSRAIKPFAEPGRPPDWFSQKHCASQYSELLETTETPKRKRGEKGEVVETVEDVIVRKLTAERVEELKKVIKETQERYRRLKRDAELIQAGHMDSRLDELCNDIATKKKLEEEEAEVKRKATDAAYQARQAVKTPPRRLPTVMVRSPIDSASPGGDYPLGDLTPTTMEEATSGVNESEMAVASGHLNSTGVLLEVGGVLPMIHGGEIQQTPNTVAASPAASGAPTLSRLLEAGPTQFTTPLASFTTVASEPPVKLVPPPVESVSQATIVMMPALPAPSSAPAVSTTESVAPVSQPDNCVPMEAVGDPHTVTVSMDSSEISMIINSIKEECFRSGVAEAPVGSKAPSIDGKEELDLAEKMDIAVSYTGEELDFETVGDIIAIIEDKVDDHPEVLDVAAVEAALSFCEENDDPQSLPGPWEHPIQQERDKPVPLPAPEMTVKQERLDFEETENKGIHELVDIREPSAEIKVEPAEPEPVISGAEIVAGVVPATSMEPPELRSQDLDEELGSTAAGEIVEADVAIGKGDETPLTNVKTEASPESMLSPSHGSNPIEDPLEAETQHKFEMSDSLKEESGTIFGSQIKDAPGEDEEEDGVSEAASLEEPKEEDQGEGYLSEMDNEPPVSESDDGFSIHNATLQSHTLADSIPSSPASSQFSVCSEDQEAIQAQKIWKKAIMLVWRAAANHRYANVFLQPVTDDIAPGYHSIVQRPMDLSTIKKNIENGLIRSTAEFQRDIMLMFQNAVMYNSSDHDVYHMAVEMQRDVLEQIQQFLATQLIMQTSESGISAKSLRGRDSTRKQDASEKDSVPMGSPAFLLSLFMGHEWVWLDSEQDHPNDSELSNDCRSLFSSWDSSLDLDVGNWRETEDPEAEELEESSPEREPSELLVGDGGSEESQEAARKASHQNLLHFLSEVAYLMEPLCISSNESSEGCCPPSGTRQEGREIKASEGERELCRETEELSAKGDPLVAEKPLGENGKPEVASAPSVICTVQGLLTESEEGEAQQESKGEDQGEVYVSEMEDQPPSGECDDAFNIKETPLVDTLFSHATSSKLTDLSQDDPVQDHLLFKKTLLPVWKMIASHRFSSPFLKPVSERQAPGYKDVVKRPMDLTSLKRNLSKGRIRTMAQFLRDLMLMFQNAVMYNDSDHHVYHMAVEMRQEVLEQIQVLNIWLDKRKGSSSLEGEPANPVDDGKPVF
预测分子量	kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于BRD8重组蛋白的3篇参考文献摘要(基于公开研究内容概括，具体文献需核实)：

1. **文献名称**：*Structural and functional characterization of the recombinant BRD8 bromodomain*

**作者**：Smith A, et al.

**摘要**：该研究利用大肠杆菌表达系统成功制备了重组BRD8溴结构域蛋白，并通过X射线晶体学解析其三维结构，揭示了其与乙酰化组蛋白结合的关键氨基酸位点，为靶向药物设计提供基础。

2. **文献名称**：*BRD8 regulates transcriptional networks in cancer via recombinant protein-mediated chromatin binding*

**作者**：Li X, et al.

**摘要**：作者通过哺乳动物细胞表达纯化BRD8重组蛋白，结合ChIP-seq技术证明其在卵巢癌细胞中通过结合特定乙酰化修饰的染色质区域调控致癌基因表达，提示其作为治疗靶点的潜力。

3. **文献名称**：*Development of a BRD8 recombinant protein-based assay for high-throughput inhibitor screening*

**作者**：Zhang Y, et al.

**摘要**：研究团队优化了BRD8重组蛋白的体外表达与纯化流程，并基于该蛋白建立了一种荧光偏振检测方法，用于高通量筛选靶向BRD8溴结构域的小分子抑制剂，发现多个先导化合物。

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**注**：以上为模拟概括性内容，实际文献需通过PubMed、Google Scholar等平台以“BRD8 recombinant protein”“BRD8 bromodomain”等关键词检索获取。

背景信息

Bromodomain-containing protein 8 (BRD8) is a member of the bromodomain and extraterminal (BET) family, which includes structurally conserved epigenetic readers that bind acetylated lysine residues on histones and other proteins. BRD8 contains two tandem bromodomains that enable its interaction with acetylated chromatin, playing a role in transcriptional regulation and chromatin remodeling. Unlike other BET proteins (e.g., BRD2. BRD3. BRD4), BRD8 is less characterized but has garnered interest due to its unique functional associations and potential therapeutic implications.

Recombinant BRD8 protein is engineered in vitro using expression systems like *E. coli* or mammalian cells, enabling researchers to study its biochemical properties, structure, and interactions. Studies suggest BRD8 participates in the SWI/SNF chromatin-remodeling complex, influencing gene expression by modulating chromatin accessibility. It also interacts with the tumor suppressor p53. regulating its stability and activity, which links BRD8 to cancer pathways. Dysregulation of BRD8 has been implicated in various cancers, including glioblastoma and ovarian cancer, where it may promote cell proliferation or chemoresistance.

The development of recombinant BRD8 facilitates drug discovery, particularly for BET inhibitors targeting bromodomain-acetyl-lysine interactions. While most BET inhibitors focus on BRD4. BRD8-specific probes could reveal its distinct biological roles. Structural studies using recombinant BRD8 have mapped critical residues for acetyl-lysine binding, aiding in designing selective inhibitors. Additionally, BRD8's role in viral replication (e.g., HIV) and cellular stress responses highlights its broader relevance in disease mechanisms.

Despite progress, BRD8's full functional spectrum remains elusive. Recombinant protein tools are essential for unraveling its interactions with histone variants, non-histone partners, and regulatory networks, offering insights into epigenetic therapies and precision oncology.

重组蛋白表达纯化服务

在生物科技领域，蛋白研发与生产是前沿探索的关键支撑。艾普蒂作为行业内的创新者，凭借自身卓越的研发实力，每年能成功研发 1000 多种全新蛋白，在重组蛋白领域不断突破。在重组蛋白生产过程中，艾普蒂积累了丰富且成熟的经验。从结构复杂的跨膜蛋白，到具有特定催化功能的酶、参与信号传导的激酶，再到用于免疫研究的病毒抗原，艾普蒂都能实现高效且稳定的生产。这一成就离不开艾普蒂强大的技术平台。我们构建了多元化的重组蛋白表达系统，昆虫细胞、哺乳动物细胞以及原核蛋白表达系统协同运作。不同的表达系统各有优势，能够满足不同客户对重组蛋白的活性、产量、成本等多样化的需求，从而提供高品质、低成本的活性重组蛋白。艾普蒂提供的不只是产品，更是从源头到终端的一站式解决方案。从最初的基因合成，精准地构建出符合要求的基因序列，到载体构建，为蛋白表达创造适宜的环境，再到蛋白质表达和纯化，每一个环节都严格把控。我们充分尊重客户的个性化需求，在表达 / 纯化标签的选择、表达宿主的确定等方面，为客户量身定制专属方案。同时，艾普蒂还配备了多种纯化体系，能够应对不同特性蛋白的纯化需求。这种灵活性和专业性，极大地提高了蛋白表达和纯化的成功率，让客户的研究项目得以顺利推进，在生物科技的探索道路上助力每一位科研工作者迈向成功。

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技术平台

艾普蒂生物自主研发并建立综合性重组蛋白生产和抗体开发技术平台，包括：哺乳动物细胞表达平台：利用哺乳动物细胞精准修饰蛋白，产出与天然蛋白相似的重组蛋白，用于药物研发、细胞治疗等。杂交瘤开发平台：通过细胞融合筛选出稳定分泌单克隆抗体的杂交瘤细胞株，优化后的技术让抗体亲和力与特异性更高，应用于疾病诊断、免疫治疗等领域。单 B 细胞筛选平台：FACS 用荧光标记和流式细胞仪快速分选特定 B 细胞；Beacon® 基于微流控技术，单细胞水平捕获、分析 B 细胞，挖掘抗体多样性，缩短开发周期。凭借这些平台，艾普蒂生物为客户提供优质试剂和专业 CRO 技术服务，推动生物科技发展。

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蛋白工艺开发

艾普蒂生物在重组蛋白和天然蛋白开发领域经验十分丰富，拥有超过 2 万种重组蛋白的开发案例。在四大重组蛋白表达平台的运用上，艾普蒂生物不仅经验老到，还积累了详实的成功案例。针对客户的工业化生产需求，我们能够定制并优化实验方案。通过小试探索、工艺放大以及条件优化等环节，对重组蛋白基因序列进行优化，全面探索多种条件，精准找出最契合客户需求的生产方法。此外，公司还配备了自有下游验证平台，可对重组蛋白展开系统的质量检测与性能测试，涵盖蛋白互作检测、活性验证、内毒素验证等，全方位保障产品质量。卡梅德生物同样重视蛋白工艺开发，确保生产出的蛋白质具备所需的纯度、稳定性与生物活性，这对于保障药物的安全性和有效性起着关键作用，与艾普蒂生物共同推动着行业的发展。

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