| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CENPH |
| Uniprot No | Q9H3R5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 136-247aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMLNKLIMKSQQESWDLEEKLLDI RKKRL QLKQASESKLLEIQTEKNKQKIDLDSMENSERIKIIRQNLQMEIK ITT VIQHVFQNLILGSKVNWAEDPALKEIVLQLEKNVDMM |
| 预测分子量 | 16 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于CENPH重组蛋白的参考文献及其摘要概括：
1. **文献名称**: *"Recombinant Human CENPH Protein: Expression, Purification, and Interaction with Centromere Complex"*
**作者**: Li, X., et al.
**摘要**: 本研究利用大肠杆菌表达系统成功克隆并纯化了重组人CENPH蛋白。通过体外结合实验证实其与CENP-A和CENP-C的相互作用，表明CENPH在着丝粒复合体组装中起关键作用，为研究染色体分离机制提供了蛋白工具。
2. **文献名称**: *"Functional Characterization of CENPH in Mitotic Progression Using Recombinant Protein Mutants"*
**作者**: Tanaka, K., & Nakamura, Y.
**摘要**: 文章通过昆虫细胞表达系统制备了重组CENPH及其功能缺失突变体，发现CENPH的N端结构域对微管结合至关重要。实验证明CENPH突变导致HeLa细胞有丝分裂延迟，提示其在维持染色体稳定性中的直接作用。
3. **文献名称**: *"Structural Insights into CENPH’s Role in Kinetochore Assembly via Recombinant Protein Crystallography"*
**作者**: Wang, Q., et al.
**摘要**: 该研究解析了重组CENPH蛋白的晶体结构，发现其C端α螺旋结构域与CENP-T存在特异性结合。体外重构实验表明，CENPH是连接着丝粒DNA与微管附着模块的关键支架蛋白，为癌症治疗靶点开发提供结构基础。
注：上述文献信息为示例性概括，实际研究中建议通过PubMed或Web of Science以“CENPH recombinant protein”为关键词检索最新论文获取准确数据。
CENPH (Centromere Protein H) is a critical component of the constitutive centromere-associated network (CCAN), which plays a fundamental role in kinetochore assembly and chromosome segregation during mitosis and meiosis. As an essential part of the inner kinetochore, CENPH contributes to the structural and functional integrity of the centromere, ensuring proper microtubule attachment and accurate chromosomal distribution to daughter cells. Its interaction with other centromeric proteins, such as CENP-A, CENP-C, and CENP-T, stabilizes the kinetochore-microtubule interface and regulates checkpoint signaling to prevent aneuploidy—a hallmark of genomic instability linked to cancer and developmental disorders.
Recombinant CENPH protein, produced via heterologous expression systems (e.g., E. coli or mammalian cells), enables detailed biochemical and functional studies. It typically retains conserved domains necessary for centromere localization and protein-protein interactions, allowing researchers to dissect its role in kinetochore dynamics, cell cycle regulation, and epigenetic maintenance of centromeric chromatin. Engineered versions with tags (e.g., His, GFP) facilitate purification, localization tracking, or interaction mapping.
Studies using recombinant CENPH have advanced understanding of mitotic errors in diseases, particularly cancers exhibiting chromosomal instability. Additionally, it serves as a tool for screening therapeutic agents targeting cell division machinery. Despite progress, unresolved questions remain about post-translational modifications regulating CENPH activity and its tissue-specific roles, underscoring the continued relevance of recombinant protein-based approaches in centromere biology research.
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