| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SCAND3 |
| Uniprot No | Q6R2W3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1325aa |
| 氨基酸序列 | MEAVSRVFPALAGQAPEEQGEIIKVKVKEEDHTWDQESALRRNLSYTRELSRQRFRQFCYQETPGPREALSQLRELCRQWLNPEIHTKEQILELLVLEQFLTILPEELQSWVREHNPESGEEVVTLLEDLERELDEPRQQVSQGTYGQEVSMEEMIPLDSAKESLGTQLQSMEDRMECESPEPHPLQDNGSFLWFSMMSQSMGGDNLSSLDTNEAEIEPENMREKFFRSLARLLENKSNNTKIFSKAKYCQLIKEVKEAKAKAKKESVDYRRLARFDVILVQGNEKLIEAVNGETDKIRYYLHSEDLFDILHNTHLSIGHGGRTRMEKELQAKYKNITKEVIMLYLTLCKPCQQKNSKLKKVLTSKSIKEVSSRCQVDLIDMQLNPDGEYRFILHYQDLCTKLTFLRSLKSKRPTEVAHALLDIFTIIGAPSVLQSDNGREFSSQVVSELSNIWPELKIVHGKSQTCQSQSSAEQTEDIRKRIFSWMQTNNSSHWTEFLWFIQMSQNQPYHRSMQQTPCESAFSSEAKLGLSHSQLTEELVASLHTENELDQADKELENTLRAQYEENIETGTDSSDIEENLSVTPKVAEKSPPESRLRFLSCVVCEKECTGVNSCISCDGNIHAICGVPSQHGTEGCGRQITCSLCYETSTMKRKHDEIQRSLPVKPSKMLKPSGTPFSPDKVGDWMAKQASLDFFVKKRHAFSEHSSSNKRNVNNRSYPEEGKTKRVHASFTRKYDPSYIEFGFVAVIDGEVLKPQCIICGDVLANEAMKPSKLKRHLYSKHKEISSQPKEFFERKSSELKSQPKQVFNVSHINISALRASYKVALPVAKSKTPYTIAETLVKDCIKEVCLEMLGESAAKKVAQVPLSNDTIARRIQELANDMEDQLIEQIKLAKYFSLQLDECRDIANMIILLVYVRFEHDDDIKEEFFFSASLPTNTTSSELYEAVKNYIVNKCGLEFKFCVGVCSDGAASMTGKHSEVVTQIKELAPECKTTHCFIHRESLAMKKISAELNSVLNDIVKIVNYIKSNSLNSRLFSLLCDNMEADHKQLLLHAEIRWLSRGKVLSRMFEIRNELLVFLQGKKPMWSQLFKDVNWTARLAYLSDIFSIFNDLNASMQGKNATYFSMADKVEGQKQKLEAWKNRISTDCYDMFHNLTTIINEVGNDLDIAHLRKVISEHLTNLLECFEFYFPSKEDPRIGNLWIQNPFLSSKDNLNLTVTLQDKLLKLATDEGLKISFENTASLPSFWIKAKNDYPELAEIALKLLLLFPSTYLCETGFSTLSVIKTKHRNSLNIHYPLRVALSSIQPRLDKLTSKKQAHLSH |
| 预测分子量 | 151,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SCAND3重组蛋白的假设性参考文献示例，供参考(请注意实际文献需通过学术数据库验证)：
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1. **"Efficient Expression and Purification of Recombinant SCAND3 Protein in Mammalian Cells"**
*Author: Zhang Y, et al.*
**摘要**：本研究成功构建了SCAND3的重组表达载体，在HEK293细胞中高效表达并纯化出可溶性SCAND3蛋白，验证了其与特定DNA序列的结合能力，为功能研究奠定基础。
2. **"SCAND3 Recombinant Protein Inhibits Adipogenesis via Epigenetic Regulation"**
*Author: Kim S, et al.*
**摘要**：通过体外实验证明，重组SCAND3蛋白通过结合染色质修饰复合物，抑制脂肪细胞分化关键基因的转录，揭示了其在代谢调控中的潜在作用。
3. **"Crystal Structure of the SCAN Domain of SCAND3 Reveals Dimerization Mechanism"**
*Author: Thompson R, et al.*
**摘要**：利用重组SCAND3蛋白的SCAN结构域解析其晶体结构，发现其通过二聚化参与转录调控，突变实验证实关键氨基酸对功能的影响。
4. **"Development of a SCAND3 Recombinant Protein-Based Assay for High-Throughput Screening"**
*Author: Patel M, et al.*
**摘要**：开发基于重组SCAND3蛋白的荧光偏振检测方法，用于筛选调控其活性的小分子化合物，助力药物发现研究。
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**注意**：以上内容为模拟示例，实际文献需通过PubMed、Google Scholar等平台检索关键词(如“SCAND3 recombinant protein”“SCAND3 expression”)获取。若研究较少，建议扩展检索至SCAN结构域相关蛋白家族(如SCAND1/2)。
SCAND3 (SCAN domain-containing protein 3) is a member of the SCAN protein family, characterized by a conserved SCAN domain that mediates protein-protein interactions and plays roles in transcriptional regulation. The SCAN domain, found in zinc finger-containing proteins, is critical for oligomerization and binding to chromatin modifiers or transcription factors. SCAND3. also known as RAZIN or SDP1. lacks intrinsic DNA-binding zinc fingers but regulates gene expression by interacting with other SCAN-zinc finger proteins or chromatin-associated complexes. It is implicated in modulating epigenetic processes, such as DNA methylation and histone modification, thereby influencing cellular differentiation, proliferation, and apoptosis.
Recombinant SCAND3 proteins are engineered to study its structural and functional properties. Produced via expression systems like *E. coli* or mammalian cells, these proteins retain the SCAN domain's ability to dimerize and recruit partners, enabling investigations into its role in gene silencing, chromatin remodeling, and tumor suppression. Studies suggest SCAND3 acts as a tumor suppressor in certain cancers, with reduced expression linked to poor prognosis. Its interaction with proteins like KAP1 (TRIM28) highlights its involvement in maintaining heterochromatin stability and repressing retrotransposons.
Research on recombinant SCAND3 aids in deciphering its molecular mechanisms, including its potential as a therapeutic target or biomarker in diseases like leukemia and solid tumors. Further exploration of its regulatory networks could uncover novel pathways in epigenetics and cancer biology.
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