| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NONO |
| Uniprot No | Q15233 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-471aa |
| 氨基酸序列 | MQSNKTFNLEKQNHTPRKHHQHHHQQQHHQQQQQQPPPPPIPANGQQASSQNEGLTIDLKNFRKPGEKTFTQRSRLFVGNLPPDITEEEMRKLFEKYGKAGEVFIHKDKGFGFIRLETRTLAEIAKVELDNMPLRGKQLRVRFACHSASLTVRNLPQYVSNELLEEAFSVFGQVERAVVIVDDRGRPSGKGIVEFSGKPAARKALDRCSEGSFLLTTFPRPVTVEPMDQLDDEEGLPEKLVIKNQQFHKEREQPPRFAQPGSFEYEYAMRWKALIEMEKQQQDQVDRNIKEAREKLEMEMEAARHEHQVMLMRQDLMRRQEELRRMEELHNQEVQKRKQLELRQEEERRRREEEMRRQQEEMMRRQQEGFKGTFPDAREQEIRMGQMAMGGAMGINNRGAMPPAPVPAGTPAPPGPATMMPDGTLGLTPPTTERFGQAATMEGIGAIGGTPPAFNRAAPGAEFAPNKRRRY |
| 预测分子量 | 67.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NONO重组蛋白的3篇文献概括，基于学术研究主题模拟整理：
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1. **文献名称**：*Functional characterization of recombinant NONO protein in RNA splicing regulation*
**作者**：Li, X. et al.
**摘要**：本研究通过在大肠杆菌系统中表达并纯化重组NONO蛋白，探究其在RNA剪接中的分子机制。实验表明，重组NONO能够直接结合pre-mRNA，并通过与剪接体组分相互作用调控选择性剪接过程，为NONO在转录后调控中的功能提供证据。
2. **文献名称**：*Structural insights into the dimerization of NONO protein via recombinant expression and crystallography*
**作者**：Zhang, Y. et al.
**摘要**：利用重组表达技术获得人源NONO蛋白，并通过X射线晶体学解析其二聚化结构域的三维结构。研究发现，NONO的N端结构域是其形成同源二聚体的关键，这一特性对其参与核斑点形成及RNA代谢至关重要。
3. **文献名称**：*Recombinant NONO as a potential biomarker in breast cancer progression*
**作者**：Wang, H. et al.
**摘要**：通过体外重组表达NONO蛋白，分析其在乳腺癌细胞系中的异常表达模式。功能实验表明，NONO重组蛋白的过表达可促进癌细胞增殖和侵袭，提示其可能作为乳腺癌治疗的潜在靶点。
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以上内容为示例性概括，具体文献需通过学术数据库检索确认。如需实际文献，建议检索关键词“NONO recombinant protein”或“NONO protein function”获取最新研究。
**Background of NONO Recombinant Protein**
NONO (Non-POU domain-containing octamer-binding protein), also known as p54nrb, is a multifunctional RNA/DNA-binding protein belonging to the Drosophila behavior/human splicing (DBHS) family, which includes proteins like SFPQ and PSPC1. It plays critical roles in transcriptional regulation, RNA processing, and DNA repair. Structurally, NONO contains RNA recognition motifs (RRMs) and coiled-coil domains, enabling interactions with nucleic acids and partner proteins. It is involved in diverse cellular processes, such as splicing, nuclear retention of RNA, and modulation of transcriptional pause-release.
NONO's recombinant form is engineered for research to study its molecular mechanisms and interactions. Recombinant NONO protein is typically produced using expression systems like *E. coli* or mammalian cells, followed by purification to ensure high specificity and activity. Its applications span *in vitro* assays (e.g., protein-RNA/DNA binding studies), structural analyses, and functional investigations in diseases.
Notably, NONO is implicated in cancer, neurodevelopmental disorders, and viral infections. For example, it interacts with viral RNAs to regulate replication and participates in DNA damage response pathways. Dysregulation of NONO is linked to tumor progression, neurological conditions, and circadian rhythm disruptions. Recombinant NONO aids in exploring these pathways, offering insights into therapeutic targeting.
Studies also highlight its role in paraspeckle formation, subnuclear bodies involved in gene expression regulation. By leveraging recombinant NONO, researchers dissect its contributions to cellular stress responses and disease pathogenesis, positioning it as a potential biomarker or drug target.
In summary, recombinant NONO protein serves as a vital tool for elucidating the multifaceted roles of this DBHS family member in health and disease, bridging molecular biology with translational research.
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