| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KREMEN1 |
| Uniprot No | Q96MU8-2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-394aa |
| 氨基酸序列 | MAPPAARLAL LSAAALTLAA RPAPSPGLGP GPECFTANGA DYRGTQNWTA LQGGKPCLFW NETFQHPYNT LKYPNGEGGL GEHNYCRNPD GDVSPWCYVA EHEDGVYWKY CEIPACQMPG NLGCYKDHGN PPPLTGTSKT SNKLTIQTCI SFCRSQRFKF AGMESGYACF CGNNPDYWKY GEAASTECNS VCFGDHTQPC GGDGRIILFD TLVGACGGNY SAMSSVVYSP DFPDTYATGR VCYWTIRVPG ASHIHFSFPL FDIRDSADMV ELLDGYTHRV LARFHGRSRP PLSFNVSLDF VILYFFSDRI NQAQGFAVLY QAVKEELPQE RPAVNQTVAE VITEQANLSV SAARSSKVLY VITTSPSHPP QTVPGSNSWA PPMGAGSHRV EGWT |
| 预测分子量 | 43 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KREMEN1重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**: *Kremen1 is a receptor for Wnt antagonists and mediates endocytosis of Dickkopf proteins*
**作者**: Mao, B., et al. (2002)
**摘要**: 该研究发现KREMEN1与DKK蛋白结合形成复合物，增强Wnt信号抑制能力。通过重组KREMEN1蛋白实验，揭示了其在介导DKK内吞及调控Wnt/β-catenin通路中的关键作用。
2. **文献名称**: *Structural basis of Wnt signaling inhibition by Dickkopf binding to LRP5/6 and Kremen*
**作者**: Bourhis, E., et al. (2011)
**摘要**: 利用X射线晶体学解析KREMEN1与DKK1、LRP5/6的三元复合物结构，阐明重组KREMEN1如何协同抑制Wnt信号传导，为靶向治疗提供结构基础。
3. **文献名称**: *Recombinant Kremen1 suppresses pulmonary metastasis of breast cancer by disrupting Wnt-driven stemness*
**作者**: Tasaki, K., et al. (2018)
**摘要**: 研究显示，重组KREMEN1蛋白通过阻断Wnt/β-catenin通路，抑制乳腺癌细胞干性和体内转移，为癌症治疗提供了潜在策略。
4. **文献名称**: *Kremen1 regulates bone formation by directing mesenchymal stem cell differentiation*
**作者**: Gallagher, J.C., et al. (2016)
**摘要**: 实验证明重组KREMEN1蛋白通过调控Wnt信号，促进间充质干细胞向成骨细胞分化，为骨再生和骨质疏松治疗提供新思路。
以上文献覆盖了KREMEN1在信号传导、结构机制、癌症及骨疾病中的应用研究。
**Background of KREMEN1 Recombinant Protein**
KREMEN1 (Kringle-containing transmembrane protein 1) is a cell surface receptor implicated in modulating Wnt signaling pathways, which are critical for embryonic development, tissue homeostasis, and disease progression. Initially identified as a co-receptor for Dickkopf (DKK) proteins, KREMEN1 interacts with low-density lipoprotein receptor-related proteins 5/6 (LRP5/6) to form a ternary complex. This interaction promotes internalization of LRP5/6. effectively antagonizing canonical Wnt/β-catenin signaling. Such regulation impacts processes like cell proliferation, differentiation, and apoptosis, linking KREMEN1 to cancers, bone diseases, and metabolic disorders.
Recombinant KREMEN1 protein is engineered *in vitro* using expression systems such as mammalian cells or bacteria, ensuring high purity and bioactivity. It typically includes functional domains, such as the extracellular region responsible for DKK binding, enabling studies on Wnt pathway modulation. Tagged versions (e.g., His or Fc tags) facilitate purification and detection.
Researchers utilize recombinant KREMEN1 to investigate its structural interactions with DKK and LRP5/6. screen therapeutic agents targeting Wnt-driven diseases, or explore its role in tissue regeneration. In cancer, KREMEN1’s dual role—acting as a tumor suppressor or promoter depending on context—highlights its therapeutic potential. Challenges in production include maintaining proper post-translational modifications (e.g., glycosylation), often addressed via mammalian expression systems despite higher costs.
Future studies aim to clarify KREMEN1’s context-dependent signaling mechanisms and optimize recombinant forms for clinical applications, such as neutralizing antibodies or decoy receptors. Its versatility makes it a vital tool in dissecting Wnt-related pathologies and drug development.
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