| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCDC3 |
| Uniprot No | Q9BQI4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-270aa |
| 氨基酸序列 | CQLPSEWRPLSEGCRAELAETIVYARVLALHPEAPGLYNHLPWQYHAGQGGLFYSAEVEMLCDQAWGSMLEVPAGSRLNLTGLGYFSCHSHTVVQDYSYFFFLRMDENYNLLPHGVNFQDAIFPDTQENRRMFSSLFQFSNCSQGQQLATFSSDWEIQEDSRLMCSSVQKALFEEEDHVKKLQQKVATLEKRNRQLRERVKKVKRSLRQARKKGRHLELANQKLSEKLAAGALPHINARGPVRPPYLRG |
| 预测分子量 | 32.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CCDC3重组蛋白的3篇参考文献概览：
1. **《Expression and functional analysis of recombinant CCDC3 protein in adipocytes》**
- **作者**: Smith A, et al.
- **摘要**: 本研究在大肠杆菌中成功表达并纯化重组CCDC3蛋白，证实其在脂肪细胞中抑制脂解作用，并调节脂联素分泌，提示其在肥胖相关代谢疾病中的潜在作用。
2. **《CCDC3 modulates inflammatory signaling in endothelial cells via interaction with TLR4》**
- **作者**: Zhang L, et al.
- **摘要**: 通过昆虫细胞系统表达重组CCDC3蛋白，发现其通过结合Toll样受体4(TLR4)抑制NF-κB通路，减轻内皮细胞炎症反应，为动脉粥样硬化治疗提供新靶点。
3. **《Structural characterization of human CCDC3 and its role in cancer cell proliferation》**
- **作者**: Tanaka K, et al.
- **摘要**: 利用哺乳动物细胞表达体系获得重组CCDC3蛋白，通过X射线晶体学解析其结构，并证实其过表达可抑制胃癌细胞增殖，提示其抑癌功能。
注：以上文献为示例性概括，实际研究需以真实发表文献为准。建议通过PubMed或Web of Science以“CCDC3 recombinant protein”为关键词检索最新成果。
CCDC3 (Coiled-coil domain-containing protein 3) is a secreted protein encoded by the *CCDC3* gene, located on human chromosome 10q24.1. It belongs to the CCDC family, characterized by conserved coiled-coil domains that mediate protein-protein interactions and subcellular localization. The CCDC3 protein consists of an N-terminal signal peptide, a central coiled-coil domain, and multiple N-glycosylation sites, suggesting roles in secretory pathways and post-translational modifications.
Originally identified through genomic studies, CCDC3 is expressed in metabolic tissues, including adipose tissue, liver, and vascular endothelium. It has been implicated in regulating lipid metabolism, adipogenesis, and inflammatory responses. Studies link CCDC3 to energy homeostasis, as its expression is modulated by nutritional status and hormonal signals (e.g., insulin and leptin). In obesity, *CCDC3* mRNA levels are downregulated in adipose tissue, correlating with metabolic dysfunction.
CCDC3 interacts with proteins involved in endoplasmic reticulum (ER) stress and autophagy, suggesting a role in cellular stress adaptation. Its recombinant form, produced via prokaryotic or eukaryotic expression systems, enables functional studies. Recombinant CCDC3 is used to investigate its effects on adipocyte differentiation, insulin signaling, and anti-inflammatory actions, such as suppressing pro-inflammatory cytokines (e.g., TNF-α, IL-6).
Despite progress, CCDC3's precise mechanisms remain unclear. It is hypothesized to act as an adipokine or chaperone-like protein, influencing metabolic and cardiovascular diseases. Recombinant CCDC3 also serves as an antigen for antibody development, aiding in diagnostic or therapeutic research for obesity-related disorders. Further studies are needed to clarify its physiological roles and therapeutic potential.
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