纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | LRDD |
Uniprot No | Q9HB75 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-910aa |
氨基酸序列 | MAATVEGPELEAAAAAGDASEDSDAGSRALPFLGGNRLSLDLYPGGCQQLLHLCVQQPLQLLQVEFLRLSTHEDPQLLEATLAQLPQSLSCLRSLVLKGGQRRDTLGACLRGALTNLPAGLSGLAHLAHLDLSFNSLETLPACVLQMRGLGALLLSHNCLSELPEALGALPALTFLTVTHNRLQTLPPALGALSTLQRLDLSQNLLDTLPPEIGGLGSLLELNLASNRLQSLPASLAGLRSLRLLVLHSNLLASVPADLARLPLLTRLDLRDNQLRDLPPELLDAPFVRLQGNPLGEASPDAPSSPVAALIPEMPRLFLTSDLDSFPVTPQGCSVTLACGVRLQFPAGATATPITIRYRLLLPEPGLVPLGPHDALLSHVLELQPHGVAFQQDVGLWLLFTPPQARRCREVVVRTRNDNSWGDLETYLEEEAPQRLWAHCQVPHFSWFLVVSRPVSNACLVPPEGTLLCSSGHPGVKVIFPPGATEEPRRVSMQVVRMAGRELQALLGEPEAAVSPLLCLSQSGPPSFLQPVTVQLPLPSGITGLSLDRSRLHLLYWAPPAATWDDITAQVVLELTHLYARFQVTHFSWYWLWYTTKNCVGGLARKAWERLRLHRVNLIALQRRRDPEQVLLQCLPRNKVDATLRRLLERYRGPEPSDTVEMFEGEEFFAAFERGIDVDADRPDCVEGRICFVFYSHLKNVKEVYVTTTLDREAQAVRGQVSFYRGAVPVRVPEEAEAARQRKGADALWMATLPIKLPRLRGSEGPRRGAGLSLAPLNLGDAETGFLTQSNLLSVAGRLGLDWPAVALHLGVSYREVQRIRHEFRDDLDEQIRHMLFSWAERQAGQPGAVGLLVQALEQSDRQDVAEEVRAVLELGRRKYQDSIRRMGLAPKDPALPGSSAPQPPEPAQA |
预测分子量 | kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LRDD(PIDD)重组蛋白的3篇代表性文献的简要整理,供参考:
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1. **文献名称**: *PIDD mediates NF-κB activation in response to DNA damage*
**作者**: Tinel, A., & Tschopp, J.
**摘要**:研究LRDD(PIDD)重组蛋白在DNA损伤信号中的作用,发现其与RIPK1和NEMO形成复合物,激活NF-κB通路以促进细胞存活,揭示其在应激反应中的双重功能(促凋亡与促存活)。
2. **文献名称**: *Structural basis for proapoptotic PIDDosome assembly*
**作者**: Park, H.H., et al.
**摘要**:通过表达重组PIDD蛋白,解析其与RAIDD和caspase-2形成的PIDDosome复合体晶体结构,阐明LRDD通过死亡结构域寡聚化激活caspase-2的分子机制。
3. **文献名称**: *Recombinant PIDD expression and its role in p53-dependent apoptosis*
**作者**: Lin, Y., et al.
**摘要**:报道LRDD重组蛋白在HEK293细胞中的表达与纯化,证明其在p53调控下参与DNA损伤诱导的凋亡,并验证其与CHK2激酶的相互作用。
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**备注**:
- LRDD在文献中常被称为**PIDD**(p53-induced protein with a death domain),两者为同一蛋白。
- 建议通过PubMed或Google Scholar检索上述作者及关键词(如PIDDosome、caspase-2激活)获取原文。
- 研究多聚焦于其在DNA损伤应答中的动态调控,平衡细胞凋亡与存活信号。
**Background of LRDD Recombinant Protein**
LRDD (Leucine-Rich Repeat and Death Domain-containing protein), also known as PIDD (p53-induced protein with a death domain), is a multidomain protein involved in critical cellular processes, including apoptosis, DNA damage response, and nuclear factor-κB (NF-κB) signaling. It was initially identified as a p53 target gene, linking it to tumor suppression pathways. Structurally, LRDD contains an N-terminal leucine-rich repeat (LRR) domain, a central zinc-binding domain, and a C-terminal death domain (DD), enabling interactions with multiple signaling partners like RAIDD, RIP1. and NEMO/IKKγ.
Recombinant LRDD protein is produced using genetic engineering techniques, often expressed in *E. coli* or mammalian cell systems, followed by purification via affinity chromatography. Its recombinant form retains functional domains, making it a valuable tool for studying molecular mechanisms of cell death, DNA repair, and inflammation. Researchers utilize LRDD to reconstitute protein complexes *in vitro*, map interaction interfaces, or screen for modulators of apoptosis-related pathways.
In cancer research, LRDD's dual role in pro-survival and pro-apoptotic signaling highlights its context-dependent functions. Dysregulation of LRDD is implicated in tumor progression, chemoresistance, and genomic instability. Additionally, its involvement in PIDDosome assembly—a caspase-2-activating complex—underscores its relevance in maintaining genomic integrity. Beyond basic research, recombinant LRDD aids in developing therapeutic strategies targeting apoptosis pathways or DNA damage response in diseases like cancer and neurodegeneration.
Overall, LRDD recombinant protein serves as a pivotal reagent for dissecting complex cellular signaling networks and exploring therapeutic interventions in stress-responsive pathways.
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