| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCDC60 |
| Uniprot No | Q8IWA6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-550aa |
| 氨基酸序列 | MTKVPATKKL QSSPNSGAVR PFYASENLRQ VPDKPMKSIK YMDKEIINLK KDLIRSRFLI QSVKIGRGYF AILREETAKK KKQQQLQKLK EEERNKFQPA EKISEIHYGD TLLSTYDDEK LKTLGARVTR RPFTPIHSCI ISPSLTEAHV EPLFRQLCAL HWLLEALTID HTHHTMKPVI TCWNPKDPGG SKSTIKKINK DKSMGQKWEH FITAPKTKKF KIPTMRVTNR KPSRRGSTLS LSRASGGSSP QSSMISVNPG SDEPPSVNTQ VTSSKDIEDN ESSSTKPDEE PLYMNLQKLL EMVREDARRT VTIENGMQRK APSILSVLKQ NKSNSAYKEM QTTLKSSERS SSTSAESHIQ PVQKKSKNRT NCDINIHYKS GVCNTMRAKF YSVAQEAGFC LQDKMEILMK RQEERGIQKF RAFVLVSNFQ KDIAKMRHHI SVVKGDAEEI ADHWYFDLLS KLPEDLKNFR PAKKILVKLQ KFGENLDLRI RPHVLLKVLQ DLRIWELCSP DIAVAIEFVR EHIIHMPQED YISWLQSRIN IPIGPYSALR |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CCDC60重组蛋白的虚构参考文献示例(内容基于学术文献常见结构模拟，非真实文献)：
1. **文献名称**：*Recombinant CCDC60 protein regulates ciliogenesis in mammalian cells*
**作者**：Zhang Y. et al. (2021)
**摘要**：本研究成功在大肠杆菌中表达并纯化重组CCDC60蛋白，证实其通过结合微管相关蛋白促进纤毛组装，为纤毛功能障碍相关疾病机制提供新见解。
2. **文献名称**：*Structural analysis of CCDC60 reveals a novel coiled-coil domain interaction*
**作者**：Smith J.R. et al. (2019)
**摘要**：通过X射线晶体学解析重组CCDC60蛋白结构，发现其N端卷曲螺旋结构介导同源二聚化，为研究其在细胞骨架中的功能奠定结构基础。
3. **文献名称**：*CCDC60 recombinant protein interacts with SPAG16 in spermatogenesis*
**作者**：Lee H. et al. (2020)
**摘要**：利用重组CCDC60进行酵母双杂交筛选，发现其与精子发生关键蛋白SPAG16直接互作，提示其在男性生殖系统中的潜在调控作用。
4. **文献名称**：*Functional characterization of CCDC60 in Wnt/β-catenin signaling using purified recombinant protein*
**作者**：Brown K. et al. (2022)
**摘要**：通过体外实验证实重组CCDC60蛋白可增强Wnt信号通路活性，并揭示其通过稳定β-catenin复合物调控靶基因表达的分子机制。
(注：以上为模拟内容，实际文献需通过学术数据库核实。)
CCDC60 (Coiled-Coil Domain-Containing Protein 60) is a member of the CCDC family, characterized by the presence of conserved coiled-coil domains that mediate protein-protein interactions and structural organization within cells. While its exact biological role remains under investigation, CCDC60 is hypothesized to play a role in cellular processes linked to cilia formation, cell cycle regulation, or intracellular transport, given the functional associations of other CCDC proteins. Some studies suggest its potential involvement in ciliopathies, a group of disorders caused by defective ciliary function, though direct evidence remains limited.
Recombinant CCDC60 protein is engineered using heterologous expression systems, such as *E. coli* or mammalian cell lines, to produce purified protein for functional and structural studies. This involves cloning the CCDC60 gene into expression vectors, followed by induction, purification (often via affinity tags like His-tags), and validation through techniques like Western blot or mass spectrometry. Recombinant CCDC60 enables researchers to explore its interactome, post-translational modifications, and subcellular localization. It also serves as an antigen for antibody development or a tool to study disease mechanisms, particularly in contexts where ciliary dysfunction or cell cycle anomalies are implicated. Despite progress, further research is needed to clarify its precise physiological roles and therapeutic relevance.
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