| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LRRC4B |
| Uniprot No | Q9NT99 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-713aa |
| 氨基酸序列 | MARARGSPCPPLPPGRMSWPHGALLFLWLFSPPLGAGGGGVAVTSAAGGGSPPATSCPVACSCSNQASRVICTRRDLAEVPASIPVNTRYLNLQENGIQVIRTDTFKHLRHLEILQLSKNLVRKIEVGAFNGLPSLNTLELFDNRLTTVPTQAFEYLSKLRELWLRNNPIESIPSYAFNRVPSLRRLDLGELKRLEYISEAAFEGLVNLRYLNLGMCNLKDIPNLTALVRLEELELSGNRLDLIRPGSFQGLTSLRKLWLMHAQVATIERNAFDDLKSLEELNLSHNNLMSLPHDLFTPLHRLERVHLNHNPWHCNCDVLWLSWWLKETVPSNTTCCARCHAPAGLKGRYIGELDQSHFTCYAPVIVEPPTDLNVTEGMAAELKCRTGTSMTSVNWLTPNGTLMTHGSYRVRISVLHDGTLNFTNVTVQDTGQYTCMVTNSAGNTTASATLNVSAVDPVAAGGTGSGGGGPGGSGGVGGGSGGYTYFTTVTVETLETQPGEEALQPRGTEKEPPGPTTDGVWGGGRPGDAAGPASSSTTAPAPRSSRPTEKAFTVPITDVTENALKDLDDVMKTTKIIIGCFVAITFMAAVMLVAFYKLRKQHQLHKHHGPTRTVEIINVEDELPAASAVSVAAAAAVASGGGVGGDSHLALPALERDHLNHHHYVAAAFKAHYSSNPSGGGCGGKGPPGLNSIHEPLLFKSGSKENVQETQI |
| 预测分子量 | 76,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LRRC4B重组蛋白的3篇参考文献概览(文献标题和内容为模拟生成，仅供参考)：
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1. **文献名称**：*LRRC4B重组蛋白的克隆表达及其在细胞粘附中的作用*
**作者**：Zhang Y, et al.
**摘要**：本研究通过基因克隆技术成功表达了LRRC4B重组蛋白，并验证其在HeLa细胞中的定位。实验表明，LRRC4B通过调控整合素信号通路参与细胞间粘附和迁移，可能为肿瘤转移研究提供新靶点。
2. **文献名称**：*LRRC4B重组蛋白的结构解析与功能表征*
**作者**：Wang L, et al.
**摘要**：利用X射线晶体学解析了LRRC4B重组蛋白的3D结构，发现其富含亮氨酸重复结构域(LRR)介导了与胞外基质的相互作用。体外实验证实，该蛋白对神经元突触形成具有调控作用。
3. **文献名称**：*LRRC4B重组蛋白在阿尔茨海默病模型中的表达异常*
**作者**：Chen X, et al.
**摘要**：通过构建LRRC4B重组蛋白的过表达和敲低模型，发现其表达水平与β-淀粉样蛋白沉积呈负相关，提示LRRC4B可能通过调节tau蛋白磷酸化参与神经退行性疾病进程。
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**说明**：以上内容为示例性模拟，实际文献需通过PubMed、Web of Science或Google Scholar等平台检索关键词“LRRC4B recombinant protein”或“LRRC4B function”获取。建议优先选择近5年发表的、高被引或发表于权威期刊(如*Nature*、*Cell*子刊等)的研究。
LRRC4B (Leucine-Rich Repeat-Containing Protein 4B) is a member of the leucine-rich repeat (LRR) protein family, characterized by tandem repeats of 20–30 amino acids rich in leucine residues. These proteins are involved in protein-protein interactions, cell signaling, and tissue development. LRRC4B, also known as Netrin-G ligand-2 (NGL-2), is primarily studied for its role in the nervous system, where it interacts with presynaptic netrin-G2 to regulate synaptic plasticity, axon guidance, and neuronal connectivity. Its extracellular LRR domain mediates specific binding, while the intracellular domain links to downstream signaling pathways.
Beyond neuroscience, LRRC4B has been implicated in cancer biology. Studies suggest it may act as a tumor suppressor, with reduced expression observed in malignancies like gliomas and colorectal cancer. Epigenetic silencing via promoter hypermethylation is proposed as a mechanism for its downregulation in tumors. Recombinant LRRC4B protein, produced using expression systems like E. coli or mammalian cells, typically retains functional domains and post-translational modifications critical for its biological activity. This engineered protein enables researchers to study its interactions, signaling mechanisms, and therapeutic potential in disease models.
Current research focuses on elucidating LRRC4B's dual roles in neural development and oncogenesis, with recombinant variants serving as tools for structural analysis, antibody development, and high-throughput drug screening. Its emerging relevance in both neuropathologies and cancer underscores its importance as a multifunctional protein warranting further investigation.
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