| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AOC3 |
| Uniprot No | Q16853 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 27-763aa |
| 氨基酸序列 | GRGGDGGEPSQLPHCPSVSPSAQPWTHPGQSQLFADLSREELTAVMRFLTQRLGPGLVDA AQARPSDNCVFSVELQLPPKAAALAHLDRGSPPPAREALAIVFFGRQPQPNVSELVVGPL PHPSYMRDVTVERHGGPLPYHRRPVLFQEYLDIDQMIFNRELPQASGLLHHCCFYKHRGR NLVTMTTAPRGLQSGDRATWFGLYYNISGAGFFLHHVGLELLVNHKALDPARWTIQKVFY QGRYYDSLAQLEAQFEAGLVNVVLIPDNGTGGSWSLKSPVPPGPAPPLQFYPQGPRFSVQ GSRVASSLWTFSFGLGAFSGPRIFDVRFQGERLVYEISLQEALAIYGGNSPAAMTTRYVD GGFGMGKYTTPLTRGVDCPYLATYVDWHFLLESQAPKTIRDAFCVFEQNQGLPLRRHHSD LYSHYFGGLAETVLVVRSMSTLLNYDYVWDTVFHPSGAIEIRFYATGYISSAFLFGATGK YGNQVSEHTLGTVHTHSAHFKVDLDVAGLENWVWAEDMVFVPMAVPWSPEHQLQRLQVTR KLLEMEEQAAFLVGSATPRYLYLASNHSNKWGHPRGYRIQMLSFAGEPLPQNSSMARGFS WERYQLAVTQRKEEEPSSSSVFNQNDPWAPTVDFSDFINNETIAGKDLVAWVTAGFLHIP HAEDIPNTVTVGNGVGFFLRPYNFFDEDPSFYSADSIYFRGDQDAGACEVNPLACLPQAA ACAPDLPAFSHGGFSHN |
| 预测分子量 | 82 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AOC3重组蛋白的3篇代表性文献的简要总结：
1. **"Recombinant human vascular adhesion protein-1 (VAP-1/AOC3) expressed in yeast induces leukocyte adhesion in vivo"**
- **作者**: Salmi M, et al.
- **摘要**: 该研究通过酵母表达系统成功制备了重组人源VAP-1/AOC3蛋白，并验证其功能活性。实验显示，重组蛋白在小鼠模型中能够介导白细胞黏附至血管内皮，证实了AOC3在炎症反应中的关键作用。
2. **"Structural and functional characterization of recombinant human AOC3: role in glucose metabolism and diabetes"**
- **作者**: Smith AB, et al.
- **摘要**: 文章报道了通过哺乳动物细胞表达系统纯化的重组AOC3蛋白的结构解析(晶体结构)，并发现其通过调控胰岛素信号通路影响葡萄糖代谢，提示AOC3可能成为糖尿病治疗的潜在靶点。
3. **"Development of a high-throughput assay for AOC3 enzymatic activity using recombinant protein"**
- **作者**: Chen L, et al.
- **摘要**: 研究利用重组AOC3蛋白建立了基于荧光底物的高通量酶活性检测方法，用于筛选AOC3抑制剂。该方法在药物开发中显示出高灵敏度和可重复性，为抗炎或抗癌药物研究提供了工具。
**备注**：上述文献为模拟示例，实际文献需通过PubMed或Web of Science等数据库检索确认。建议使用关键词如“AOC3 recombinant protein”“VAP-1 expression”或结合具体研究方向(如疾病模型、酶活性)进行精准查询。
AOC3 (Amine Oxidase, Copper Containing 3), also known as vascular adhesion protein-1 (VAP-1) or semicarbazide-sensitive amine oxidase (SSAO), is a membrane-bound glycoprotein with dual enzymatic and adhesive functions. It belongs to the copper-containing amine oxidase family and catalyzes the oxidative deamination of primary amines, generating aldehydes, hydrogen peroxide, and ammonia. Structurally, AOC3 contains a conserved catalytic domain with a copper ion and a quinone cofactor (TPQ), essential for its enzymatic activity.
Originally identified as a mediator of leukocyte trafficking, AOC3 is predominantly expressed in vascular endothelial cells, smooth muscle cells, and adipocytes. It promotes inflammation by facilitating leukocyte adhesion and transmigration through blood vessels, linking its activity to chronic inflammatory diseases such as rheumatoid arthritis, diabetes, and liver fibrosis. Additionally, its enzymatic byproducts (e.g., reactive oxygen species) contribute to oxidative stress and tissue remodeling in pathological conditions.
Recombinant AOC3 protein is produced using expression systems like mammalian cells or *E. coli* to ensure proper post-translational modifications and functional activity. Purification typically involves affinity chromatography and quality control for enzymatic fidelity. This recombinant tool enables researchers to study AOC3’s structure-function relationships, screen inhibitors, and explore its role in disease mechanisms.
Pharmaceutically, AOC3 is a therapeutic target, with inhibitors in preclinical and clinical trials for inflammatory and metabolic disorders. Its dual role as an adhesion molecule and enzyme underscores its complexity, driving ongoing research to clarify its physiological and pathological impacts. Recombinant AOC3 thus serves as a critical reagent for both basic research and drug development.
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