| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | APPL1 |
| Uniprot No | Q9UKG1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 5-377aa |
| 氨基酸序列 | DKLPIEETLEDSPQTRSLLGVFEEDATAISNYMNQLYQAMHRIYDAQNELSAATHLTSKLLKEYEKQRFPLGGDDEVMSSTLQQFSKVIDELSSCHAVLSTQLADAMMFPITQFKERDLKEILTLKEVFQIASNDHDAAINRYSRLSKKRENDKVKYEVTEDVYTSRKKQHQTMMHYFCALNTLQYKKKIALLEPLLGYMQAQISFFKMGSENLNEQLEEFLANIGTSVQNVRREMDSDIETMQQTIEDLEVASDPLYVPDPDPTKFPVNRNLTRKAGYLNARNKTGLVSSTWDRQFYFTQGGNLMSQARGDVAGGLAMDIDNCSVMAVDCEDRRYCFQITSFDGKKSSILQAESKKDHEEWICTINNISKQI |
| 预测分子量 | 70.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于APPL1重组蛋白的3篇参考文献示例：
1. **文献名称**：*"APPL1 regulates insulin signaling through interaction with Akt2"*
**作者**：Saito T, et al.
**摘要**：研究报道APPL1重组蛋白通过直接结合Akt2激酶调控胰岛素信号通路，揭示了其在葡萄糖代谢中的作用，并证明APPL1缺失会导致胰岛素抵抗。
2. **文献名称**：*"Structural basis of APPL1 involved in adiponectin signaling"*
**作者**：Xu P, et al.
**摘要**：通过重组APPL1蛋白的晶体结构解析，阐明了其与脂联素受体结合的分子机制，为代谢性疾病治疗提供结构生物学依据。
3. **文献名称**：*"APPL1 modulates mitochondrial biogenesis through the AMPK pathway"*
**作者**：Mao X, et al.
**摘要**：利用重组APPL1蛋白验证其通过AMPK激活促进线粒体生成的机制，证明其在能量代谢和肥胖相关疾病中的调控作用。
(注：以上文献为虚拟示例，实际研究需根据具体数据库检索。)
**Background of APPL1 Recombinant Protein**
APPL1 (Adaptor Protein, Phosphotyrosine Interaction, PH Domain and Leucine Zipper Containing 1) is a multifunctional adaptor protein involved in diverse cellular signaling pathways. It was initially identified as an interacting partner of AKT2. a key kinase in insulin signaling. Structurally, APPL1 contains an N-terminal pleckstrin homology (PH) domain, a central phosphotyrosine-binding (PTB) domain, and a C-terminal leucine zipper motif, enabling interactions with membrane receptors, signaling molecules, and cytoskeletal components.
APPL1 acts as a signaling hub, linking cell surface receptors (e.g., adiponectin receptors, insulin receptors) to downstream effectors such as PI3K/AKT, MAPK, and Wnt/β-catenin pathways. It plays critical roles in regulating metabolism, cell proliferation, apoptosis, and vesicular trafficking. Notably, APPL1 is essential for adiponectin-mediated insulin sensitization and glucose uptake, linking it to metabolic disorders like diabetes and obesity. Dysregulation of APPL1 expression or function is also implicated in cancer progression, neurodegenerative diseases, and cardiovascular conditions.
Recombinant APPL1 protein is typically produced in *E. coli* or mammalian expression systems, often fused with tags (e.g., His, GST) for purification. It serves as a vital tool for *in vitro* studies, enabling researchers to dissect APPL1’s interactions, post-translational modifications, and mechanistic roles in signaling cascades. Its applications extend to drug discovery, particularly in targeting metabolic or oncogenic pathways.
Overall, APPL1 recombinant protein is pivotal for advancing our understanding of cellular signaling cross-talk and developing therapeutics for diseases linked to APPL1 dysregulation.
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