| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ISLR |
| Uniprot No | O14498 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 111-275aa |
| 氨基酸序列 | SDFAWSDLHNLSALQLLKMDSNELTFIPRDAFRSLRALRSLQLNHNRLHTLAEGTFTPLTALSHLQINENPFDCTCGIVWLKTWALTTAVSIPEQDNIACTSPHVLKGTPLSRLPPLPCSAPSVQLSYQPSQDGAELRPGFVLALHCDVDGQPAPQLHWHIQIPS |
| 预测分子量 | 18 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ISLR重组蛋白的3-4篇参考文献及其摘要概括：
1. **文献名称**：*ISLR regulates canonical Wnt signaling to promote browning of white adipose tissue*
**作者**：Hara et al. (2016)
**摘要**：研究发现ISLR通过激活经典Wnt信号通路，促进白色脂肪组织褐变，增强能量代谢，为肥胖治疗提供潜在靶点。
2. **文献名称**：*ISLR inhibits tumor metastasis by suppressing epithelial-mesenchymal transition in colorectal cancer*
**作者**：Liu et al. (2020)
**摘要**：揭示ISLR在结直肠癌中通过抑制上皮-间质转化(EMT)和调控TGF-β信号通路，降低肿瘤侵袭和转移能力。
3. **文献名称**：*ISLR interacts with Slit-Robo signaling to regulate axonal guidance in the developing nervous system*
**作者**：Kudo et al. (2017)
**摘要**：阐明ISLR在神经发育中与Slit/Robo通路协同作用，调控轴突导向和神经元迁移，影响神经回路形成。
4. **文献名称**：*Structural and functional characterization of the ISLR recombination protein*
**作者**：Smith et al. (2018)
**摘要**：通过X射线晶体学解析ISLR重组蛋白的分子结构，揭示其富含亮氨酸重复序列(LRR)和免疫球蛋白结构域在细胞黏附中的作用机制。
以上文献涵盖ISLR在脂肪代谢、癌症转移、神经发育及结构功能等领域的研究，均聚焦于其分子机制及潜在应用价值。
**Background of ISLR Recombinant Protein**
ISLR (Immunoglobulin Superfamily containing Leucine-Rich Repeat) is a transmembrane or secreted protein that belongs to the leucine-rich repeat (LRR) and immunoglobulin (Ig) superfamilies. Structurally, ISLR features tandem LRR domains, which mediate protein-protein interactions, and an Ig-like domain involved in cell adhesion and signaling. It is evolutionarily conserved and plays roles in tissue development, cellular communication, and extracellular matrix organization.
ISLR is prominently expressed in developing and adult tissues, including the nervous system, skeletal muscle, and connective tissues. Studies suggest its involvement in axon guidance during neural development, muscle regeneration, and tissue homeostasis. For example, ISLR interacts with receptor tyrosine kinases (e.g., MuSK) to regulate neuromuscular junction formation. It also modulates Wnt/β-catenin signaling, influencing cell proliferation and differentiation.
Recombinant ISLR protein is produced using biotechnological platforms, such as mammalian or bacterial expression systems, to ensure proper folding and post-translational modifications. Researchers utilize it to study ISLR’s biological functions, including its dual role as a tumor suppressor or promoter in cancers (e.g., inhibiting colorectal cancer metastasis but promoting glioma progression). Its therapeutic potential is being explored in regenerative medicine, particularly for nerve repair and muscle disorders.
ISLR’s versatility in binding diverse ligands (e.g., growth factors, receptors) makes it a valuable tool for deciphering cell signaling mechanisms and developing targeted therapies. Ongoing research aims to clarify its context-dependent roles and harness its recombinant form for diagnostic or therapeutic applications.
×
