| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PALLD |
| Uniprot No | Q8WX93 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1383aa |
| 氨基酸序列 | MSGTSSHESFYDSLSDMQEESKNTDFFPGLSAFLSQEEINKSLDLARRAIADSETEDFDSEKEISQIFSTSPASLCEHPSHKETKLGEHASRRPQDNRSTPVQPLAEKQTKSISSPVSKRKPAMSPLLTRPSYIRSLRKAEKRGAKTPSTNVKPKTPHQRKGGPQSQLCDKAANLIEELTSIFKAAKPRNRSPNGESSSPDSGYLSPKNQPSALLSASASQSPMEDQGEMEREVKSPGARHCYQDNQDLAVPHNRKSHPQPHSALHFPAAPRFIQKLRSQEVAEGSRVYLECRVTGNPTPRVRWFCEGKELHNTPDIQIHCEGGDLHTLIIAEAFEDDTGRYTCLATNPSGSDTTSAEVFIEGASSTDSDSESLAFKSRAGAMPQAQKKTTSVSLTIGSSSPKTGVTTAVIQPLSVPVQQVHSPTSYLCRPDGTTTAYFPPVFTKELQNTAVAEGQVVVLECRVRGAPPLQVQWFRQGSEIQDSPDFRILQKKPRSTAEPEEICTLVIAETFPEDAGIFTCSARNDYGSATSTAQLVVTSANTENCSYESMGESNNDHFQHFPPPPPILETSSLELASKKPSEIQQVNNPELGLSRAALQMQFNAAERETNGVHPSRGVNGLINGKANSNKSLPTPAVLLSPTKEPPPLLAKPKLDPLKLQQLQNQIRLEQEAGARQPPPAPRSAPPSPPFPPPPAFPELAACTPPASPEPMSALASRSAPAMQSSGSFNYARPKQFIAAQNLGPASGHGTPASSPSSSSLPSPMSPTPRQFGRAPVPPFAQPFGAEPEAPWGSSSPSPPPPPPPVFSPTAAFPVPDVFPLPPPPPPLPSPGQASHCSSPATRFGHSQTPAAFLSALLPSQPPPAAVNALGLPKGVTPAGFPKKASRTARIASDEEIQGTKDAVIQDLERKLRFKEDLLNNGQPRLTYEERMARRLLGADSATVFNIQEPEEETANQEYKVSSCEQRLISEIEYRLERSPVDESGDEVQYGDVPVENGMAPFFEMKLKHYKIFEGMPVTFTCRVAGNPKPKIYWFKDGKQISPKSDHYTIQRDLDGTCSLHTTASTLDDDGNYTIMAANPQGRISCTGRLMVQAVNQRGRSPRSPSGHPHVRRPRSRSRDSGDENEPIQERFFRPHFLQAPGDLTVQEGKLCRMDCKVSGLPTPDLSWQLDGKPVRPDSAHKMLVRENGVHSLIIEPVTSRDAGIYTCIATNRAGQNSFSLELVVAAKEAHKPPVFIEKLQNTGVADGYPVRLECRVLGVPPPQIFWKKENESLTHSTDRVSMHQDNHGYICLLIQGATKEDAGWYTVSAKNEAGIVSCTARLDVYTQWHQQSQSTKPKKVRPSASRYAALSDQGLDIKAAFQPEANPSHLTLNTALVESEDL |
| 预测分子量 | 150 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PALLD(Palladin)重组蛋白的3篇示例文献(内容基于研究领域常见方向，具体文献请通过学术数据库验证)：
1. **《Palladin regulates cell migration via interactions with actin and focal adhesion kinase》**
- **作者**: Rachlin AS, et al.
- **摘要**: 研究利用重组PALLD蛋白揭示其通过结合肌动蛋白(actin)和黏着斑激酶(FAK)调控细胞迁移的分子机制，证明PALLD在细胞骨架重塑中的关键作用。
2. **《Structural characterization of the actin-binding domain of Palladin》**
- **作者**: Dixon RD, et al.
- **摘要**: 通过表达并纯化PALLD的重组蛋白片段，解析其与肌动蛋白结合的特定结构域，阐明其维持细胞极性和运动的结构基础。
3. **《Overexpression of recombinant Palladin promotes tumor invasion in pancreatic cancer models》**
- **作者**: Goicoechea SM, et al.
- **摘要**: 利用重组PALLD蛋白在胰腺癌细胞模型中验证其过表达可增强肿瘤侵袭能力，提示PALLD作为癌症转移的生物标志物潜力。
**注意**：以上为模拟示例，实际文献请通过PubMed、Google Scholar等平台以“Palladin recombinant protein”或“PALLD expression and function”为关键词检索。
Palladin (PALLD) is a cytoskeleton-associated protein encoded by the *PALLD* gene, first identified in 2000 for its upregulated expression in cancer cell lines. It plays a critical role in regulating actin cytoskeleton dynamics, cell adhesion, motility, and morphological maintenance. Structurally, palladin contains multiple immunoglobulin (Ig)-like domains that mediate interactions with other cytoskeletal components, such as α-actinin, ezrin, and vasodilator-stimulated phosphoprotein (VASP), facilitating actin filament organization and cellular scaffolding.
Recombinant PALLD proteins are engineered to study its biological functions and disease mechanisms. In physiological contexts, palladin is essential for embryonic development, cell migration, and tissue repair. However, dysregulation of PALLD is linked to pathological conditions, including cancer metastasis, fibrosis, and cardiovascular diseases. For instance, palladin overexpression in tumors promotes invasive behavior by enhancing matrix degradation and cell motility. In cardiovascular systems, PALLD mutations correlate with familial thoracic aortic aneurysms, likely due to impaired smooth muscle cell contractility.
The production of recombinant PALLD involves cloning its isoforms (e.g., 90 kDa, 140 kDa) into expression systems (e.g., *E. coli*, mammalian cells) to obtain purified, functional proteins. These reagents enable *in vitro* studies on cytoskeletal remodeling, protein-protein interactions, and signaling pathways. Additionally, recombinant PALLD serves as an antigen for antibody development or a tool for drug screening targeting cytoskeleton-related disorders. Challenges persist in elucidating isoform-specific roles and tissue-dependent expression patterns, driving ongoing research to harness PALLD’s therapeutic potential.
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