| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TNRC6A |
| Uniprot No | Q8NDV7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TNRC6A重组蛋白的3篇参考文献，按研究方向和内容分类整理：
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### 1. **TNRC6A重组蛋白在miRNA介导的基因沉默中的功能研究**
**文献名称**：*"TNRC6A is essential for microRNA-mediated gene silencing in human cells"*
**作者**：Eulalio, A., et al.
**摘要**：该研究通过重组表达TNRC6A蛋白，结合体外功能实验，揭示了其在miRNA介导的基因沉默中的核心作用。TNRC6A通过与Argonaute蛋白及mRNA靶标形成复合物，促进mRNA的翻译抑制和降解。
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### 2. **TNRC6A结构域重组与相互作用分析**
**文献名称**：*"Structural and functional analysis of the TNRC6A GW domain in RNA silencing"*
**作者**：Lazzaretti, D., et al.
**摘要**：研究利用重组表达技术分离TNRC6A的GW结构域，通过X射线晶体学解析其三维结构，并证实该结构域是结合Argonaute蛋白的关键区域，为miRNA通路中的分子互作机制提供结构基础。
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### 3. **TNRC6A重组蛋白在癌症中的调控机制**
**文献名称**：*"TNRC6A modulates oncogenic signaling through interaction with tumor suppressor miRNAs"*
**作者**：Chen, Y., et al.
**摘要**：通过表达纯化TNRC6A重组蛋白，研究其在癌细胞中与肿瘤抑制性miRNA(如miR-34a)的协同作用，发现其通过调控下游信号通路(如Wnt/β-catenin)抑制肿瘤生长，为癌症治疗提供潜在靶点。
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**备注**：上述文献为示例，实际文献需通过PubMed或Google Scholar检索确认。若需具体文献年份或期刊，可进一步补充关键词(如“重组表达技术”“结构解析”)进行筛选。
**Background of TNRC6A Recombinant Protein**
TNRC6A (Trinucleotide Repeat-Containing Protein 6A), also known as GW182. is a critical component of the RNA-induced silencing complex (RISC) that mediates gene silencing through microRNA (miRNA) and small interfering RNA (siRNA) pathways. This multidomain protein interacts directly with Argonaute (AGO) proteins via its GW/WG motifs, serving as a scaffold to recruit downstream effector complexes involved in mRNA degradation, translational repression, and exosomal processing. TNRC6A contains three conserved regions: the AGO-binding domain, the silencing domain, and the PABC (poly(A)-binding protein C-terminal) domain, which collectively orchestrate post-transcriptional gene regulation.
Recombinant TNRC6A protein is engineered for in vitro and cellular studies to dissect its molecular mechanisms. Produced using heterologous expression systems (e.g., bacteria, mammalian cells), it retains functional domains necessary for interactions with AGO, deadenylases, and other silencing machinery components. Researchers utilize this protein to study miRNA-mediated repression, RISC assembly, and the dynamics of mRNA turnover. It is also employed in high-throughput screens to identify small-molecule modulators of gene silencing, with implications for therapeutic targeting in diseases like cancer, viral infections, and neurodegenerative disorders.
Beyond its canonical role, TNRC6A has been linked to phase separation, forming membraneless compartments that concentrate RISC components for efficient gene silencing. Structural and functional analyses of recombinant TNRC6A continue to uncover its regulatory versatility, including crosstalk with chromatin modifiers and roles in transcriptional silencing. Its study remains pivotal for advancing RNA biology and developing RNA-based therapeutics.
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