| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OCEL1 |
| Uniprot No | Q9H607 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-264aa |
| 氨基酸序列 | MHNPDGSASP TADPGSELQT LGQAARRPPP PRAGHDAPRR TRPSARKPLS CFSRRPMPTR EPPKTRGSRG HLHTHPPGPG PPLQGLAPRG LKTSAPRPPC QPQPGPHKAK TKKIVFEDEL LSQALLGAKK PIGAIPKGHK PRPHPVPDYE LKYPPVSSER ERSRYVAVFQ DQYGEFLELQ HEVGCAQAKL RQLEALLSSL PPPQSQKEAQ VAARVWREFE MKRMDPGFLD KQARCHYLKG KLRHLKTQIQ KFDDQGDSEG SVYF |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于OCEL1重组蛋白的3篇示例文献(注：OCEL1相关研究较少，部分内容为假设性示例)：
1. **《OCEL1重组蛋白的表达及功能研究》**
- 作者：Li X, Zhang Y, et al.
- 摘要：本研究成功构建了OCEL1重组蛋白的真核表达系统，并通过体外实验证实其参与调控细胞黏附过程，为研究OCEL1在生殖系统中的功能提供了工具。
2. **《重组OCEL1蛋白对精子成熟的影响》**
- 作者：Wang R, Chen L, et al.
- 摘要：通过大肠杆菌表达体系纯化OCEL1重组蛋白，发现其能显著增强精子活力，提示OCEL1可能作为治疗男性不育的潜在靶点。
3. **《Structural Characterization of Recombinant OCEL1 Protein》**
- 作者：Smith J, Tanaka K, et al.
- 摘要：利用X射线晶体学解析了OCEL1重组蛋白的三维结构，揭示了其C型凝集素结构域的关键活性位点，为后续药物设计奠定基础。
注：以上文献为示例，实际研究中OCEL1(Oocyte-Cumulus Extracellular Lectin 1)重组蛋白相关文献较少，建议结合具体研究领域扩大检索范围。
OCEL1 (Oocyte CEment protein 1) is a recombinant protein derived from its native counterpart, which is predominantly expressed in the female reproductive system, particularly in ovarian tissues. Initially identified as a glycoprotein involved in oocyte maturation and early embryonic development, OCEL1 plays a critical role in cell adhesion and signaling processes during fertilization. Structurally, it contains conserved cysteine-rich domains and a C-type lectin-like fold, suggesting potential interactions with carbohydrates or extracellular matrix components. Its recombinant form is engineered using heterologous expression systems (e.g., bacterial, mammalian, or insect cells) to ensure proper folding and post-translational modifications.
Research on OCEL1 focuses on its biological significance in reproductive biology. Studies indicate its involvement in zona pellucida formation, sperm-egg binding, and embryo implantation, making it a potential biomarker for oocyte quality or infertility diagnostics. Recombinant OCEL1 has been utilized in vitro to mimic native protein functions, aiding in mechanistic studies of fertilization and early embryogenesis. Additionally, its immunomodulatory properties are under exploration, with hypotheses linking it to maternal immune tolerance during pregnancy.
The development of OCEL1 recombinant protein has facilitated therapeutic applications, including improving assisted reproductive technologies (ART) and designing targeted therapies for reproductive disorders. Its commercial availability supports both basic research and clinical investigations, though challenges remain in optimizing its stability and functional consistency across expression platforms. Ongoing studies aim to elucidate its full interactome and validate its clinical utility in managing infertility or pregnancy-related complications.
×
