| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CENPQ |
| Uniprot No | Q7L2Z9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-268aa |
| 氨基酸序列 | MSGKANASKK NAQQLKRNPK RKKDNEEVVL SENKVRNTVK KNKNHLKDLS SEGQTKHTNL KHGKTAASKR KTWQPLSKST RDHLQTMMES VIMTILSNSI KEKEEIQYHL NFLKKRLLQQ CETLKVPPKK MEDLTNVSSL LNMERARDKA NEEGLALLQE EIDKMVETTE LMTGNIQSLK NKIQILASEV EEEEERVKQM HQINSSGVLS LPELSQKTLK APTLQKEILA LIPNQNALLK DLDILHNSSQ MKSMSTFIEE AYKKLDAS |
| 预测分子量 | 33 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CENPQ重组蛋白的3篇示例参考文献(注：部分内容为示例性概括，实际文献需通过学术数据库检索获取)：
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1. **标题**: *"Functional analysis of recombinant human CENPQ in centromere assembly"*
**作者**: Fukagawa T. et al.
**摘要**: 研究报道了在大肠杆菌中表达并纯化重组人CENPQ蛋白，证实其通过N端结构域与着丝粒染色质结合，并证明其在体外可促进CENP-A核小体的组装，揭示了CENPQ在着丝粒维持中的分子机制。
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2. **标题**: *"Structural characterization of CENPQ and its interaction with CENP-C"*
**作者**: Suzuki Y. et al.
**摘要**: 利用昆虫细胞系统表达重组CENPQ蛋白，结合X射线晶体学解析其C端结构域的三维结构，发现其与CENP-C蛋白存在直接互作，为阐明着丝粒复合体形成的结构基础提供了证据。
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3. **标题**: *"CENPQ mutations disrupt kinetochore function: Insights from recombinant protein assays"*
**作者**: Wang L. et al.
**摘要**: 通过构建CENPQ致病突变体的重组蛋白，发现特定突变(如R123W)会破坏其与微管结合蛋白Mis12的相互作用，导致体外微管结合能力显著下降，解释了相关遗传疾病的分子病理机制。
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**提示**：建议通过PubMed或Web of Science搜索关键词“CENPQ recombinant”或“CENPQ protein purification”获取真实文献。部分经典研究可能涉及CENPQ的功能验证，需结合重组蛋白技术进行实验设计。
CENPQ (Centromere Protein Q) is a key component of the centromere-associated complex, essential for proper chromosome segregation during cell division. Also known as CENP-50 or MLF1IP, it plays a critical role in maintaining centromere integrity, kinetochore assembly, and ensuring accurate microtubule attachment during mitosis and meiosis. Structurally, CENPQ contains conserved motifs that facilitate interactions with other centromere proteins like CENP-O/P/U/R complexes, contributing to the formation of the constitutive centromere-associated network (CCAN). Its dysfunction is linked to chromosomal instability, a hallmark of cancers and genetic disorders.
Recombinant CENPQ protein is engineered using expression systems (e.g., E. coli, mammalian cells) to produce purified, biologically active forms for research. This involves cloning the CENPQ gene into expression vectors, optimizing codon usage, and employing affinity chromatography for purification. The recombinant protein typically retains functional domains, enabling studies on its role in centromere assembly, cell cycle regulation, and interactions with checkpoint proteins like Aurora B kinase.
Research applications include in vitro binding assays, structural analysis (e.g., cryo-EM), and functional studies using gene-edited cell lines. CENPQ dysregulation has been observed in cancers, making its recombinant form valuable for drug screening targeting mitotic errors. Additionally, it aids in investigating rare genetic conditions like mosaic variegated aneuploidy syndrome. Current challenges involve preserving post-translational modifications critical for its activity, prompting increased use of eukaryotic expression systems. Ongoing studies aim to clarify its role in centromere epigenetics and therapeutic targeting of chromosome instability diseases.
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