| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BFSP1 |
| Uniprot No | Q12934 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-665aa |
| 氨基酸序列 | MYRRSYVFQT RKEQYEHADE ASRAAEPERP ADEGWAGATS LAALQGLGER VAAHVQRARA LEQRHAGLRR QLDAFQRLGE LAGPEDALAR QVESNRQRVR DLEAERARLE RQGTEAQRAL DEFRSKYENE CECQLLLKEM LERLNKEADE ALLHNLRLQL EAQFLQDDIS AAKDRHKKNL LEVQTYISIL QQIIHTTPPA SIVTSGMREE KLLTEREVAA LRSQLEEGRE VLSHLQAQRV ELQAQTTTLE QAIKSAHECY DDEIQLYNEQ IETLRKEIEE TERVLEKSSY DCRQLAVAQQ TLKNELDRYH RIIEIEGNRL TSAFIETPIP LFTQSHGVSL STGSGGKDLT RALQDITAAK PRQKALPKNV PRRKEIITKD KTNGALEDAP LKGLEDTKLV QVVLKEESES KFESESKEVS PLTQEGAPED VPDGGQISKG FGKLYRKVKE KVRSPKEPET PTELYTKERH VLVTGDANYV DPRFYVSSIT AKGGVAVSVA EDSVLYDGQV EPSPESPKPP LENGQVGLQE KEDGQPIDQQ PIDKEIEPDG AELEGPEEKR EGEERDEESR RPCAMVTPGA EEPSIPEPPK PAADQDGAEV LGTRSRSLPE KGPPKALAYK TVEVVESIEK ISTESIQTYE ETAVIVETMI GKTKSDKKKS GEKSS |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BFSP1重组蛋白的示例参考文献(注：以下文献为示例性概括，实际文献需通过数据库验证)：
1. **《Recombinant BFSP1 expression and its role in lens transparency》**
- **作者**: Smith J, et al.
- **摘要**: 研究利用大肠杆菌系统表达重组BFSP1蛋白，分析其与晶状体α-晶状体蛋白的相互作用，揭示BFSP1在维持晶状体结构完整性中的作用。
2. **《Structural characterization of human BFSP1 using cryo-electron microscopy》**
- **作者**: Li Y, et al.
- **摘要**: 通过冷冻电镜解析重组BFSP1蛋白的纤维状结构，阐明其自组装机制及突变导致先天性白内障的分子基础。
3. **《Functional analysis of BFSP1 mutations in cataractogenesis》**
- **作者**: Zhang R, et al.
- **摘要**: 利用重组BFSP1蛋白研究临床突变体的功能异常，发现特定突变破坏蛋白稳定性，导致细胞模型中的聚集现象。
4. **《Optimization of BFSP1 purification for in vitro filament assembly assays》**
- **作者**: Kumar S, et al.
- **摘要**: 开发高效重组BFSP1纯化方案，验证其在体外自组装为中间丝的能力，为疾病相关突变体的功能研究提供技术基础。
**建议**：如需真实文献，可在PubMed或Google Scholar中搜索关键词 **"BFSP1 recombinant protein"** 或 **"BFSP1 expression and function"**，筛选近年研究。
**Background of BFSP1 Recombinant Protein**
BFSP1 (Beaded Filament Structural Protein 1), also known as filensin, is a key component of the lens-specific intermediate filament cytoskeleton in ocular tissues. It plays a critical role in maintaining the structural integrity and optical clarity of the lens by interacting with its partner protein, BFSP2 (CP49), to form beaded filaments. These filaments are unique to lens fiber cells and are essential for their elongation, organization, and resilience during lens development and aging.
Mutations in the *BFSP1* gene have been linked to congenital cataracts and other lens disorders, highlighting its importance in ocular physiology. To study its function and explore therapeutic applications, recombinant BFSP1 protein is produced using expression systems like *E. coli* or mammalian cells. This involves cloning the *BFSP1* gene into a vector, optimizing expression conditions, and purifying the protein via affinity chromatography or other techniques.
Recombinant BFSP1 enables researchers to investigate its biochemical properties, structural interactions with BFSP2. and mechanisms underlying cataract formation. It also serves as a tool for screening small molecules or gene therapies aimed at restoring lens transparency. Additionally, studies using recombinant BFSP1 contribute to understanding broader cell biology processes, such as intermediate filament dynamics and cell differentiation.
Overall, BFSP1 recombinant protein is a vital resource for advancing ocular research, bridging molecular insights with potential clinical interventions for vision-threatening disorders.
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