| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CUBN |
| Uniprot No | O60494 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CUBN(Cubilin)重组蛋白的3篇代表性文献摘要示例：
---
1. **文献名称**：*Structural insights into the function of cubilin as a high-affinity receptor for intrinsic factor-vitamin B12*
**作者**：Moestrup SK, et al.
**摘要**：该研究通过重组表达CUBN蛋白，解析其与内因子-维生素B12复合物的结合机制，揭示其依赖钙离子的配体识别结构域，为遗传性维生素B12吸收障碍提供分子基础。
---
2. **文献名称**：*Expression and characterization of recombinant cubilin in mammalian cells: Implications for proteinuria in kidney disease*
**作者**：Christensen EI, Kozyraki R.
**摘要**：利用哺乳动物细胞系统重组表达CUBN蛋白，证明其在肾小管白蛋白重吸收中的关键作用，并发现特定结构域突变导致蛋白尿的分子机制。
---
3. **文献名称**：*Cubilin dysfunction caused by mutations in the CUB domain leads to abnormal urinary protein excretion*
**作者**：Fyfe JC, et al.
**摘要**：通过重组CUBN突变体功能实验，发现CUB结构域缺陷会破坏与megalin的协同作用，导致犬类模型中的遗传性蛋白丢失性肾病，提示潜在治疗靶点。
---
注：上述内容为模拟摘要，实际文献需通过数据库(如PubMed)以“cubilin recombinant”等关键词检索核实。
**Background of CUBN Recombinant Protein**
Cubilin (CUBN), encoded by the *CUBN* gene, is a multiligand endocytic receptor critical for protein reabsorption in the kidney and intestinal nutrient uptake. Structurally, it is a large (~460 kDa) peripheral membrane protein containing 8 epidermal growth factor (EGF)-like domains and 27 CUB (complement C1r/C1s, Uegf, BMP1) domains, which mediate ligand binding. CUBN lacks transmembrane and cytosolic regions, relying on interaction with the transmembrane receptor LRP2 (megalin) for cellular internalization.
Physiologically, CUBN plays a key role in the renal proximal tubule, where it facilitates reabsorption of albumin, vitamin-binding proteins, and other filtered macromolecules. In the intestine, it mediates uptake of intrinsic factor-vitamin B12 complexes, essential for B12 absorption. Mutations in *CUBN* are linked to Imerslund-Gräsbeck syndrome, characterized by megaloblastic anemia and proteinuria.
Recombinant CUBN protein is produced using expression systems (e.g., mammalian or insect cells) to mimic its native structure and ligand-binding capacity. This engineered protein retains critical domains for studying interactions with ligands like albumin, apolipoprotein A-I, and vitamins. Researchers use it to investigate CUBN’s role in renal function, intestinal absorption, and its association with chronic kidney disease, cardiovascular disorders, or rare genetic syndromes.
In therapeutic contexts, recombinant CUBN aids in drug delivery strategies targeting kidney-specific pathways. It also serves as a tool for developing diagnostic assays for CUBN-related pathologies. Its study enhances understanding of protein handling in physiological and disease states, offering potential avenues for treating renal or metabolic disorders.
×
