| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TKL6 |
| Uniprot No | P23219 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-599aa |
| 氨基酸序列 | MSRSLLLWFLLFLLLLPPLPVLLADPGAPTPVNPCCYYPCQHQGICVRFG LDRYQCDCTRTGYSGPNCTIPGLWTWLRNSLRPSPSFTHFLLTHGRWFWE FVNATFIREMLMRLVLTVRSNLIPSPPTYNSAHDYISWESFSNVSYYTRI LPSVPKDCPTPMGTKGKKQLPDAQLLARRFLLRRKFIPDPQGTNLMFAFF AQHFTHQFFKTSGKMGPGFTKALGHGVDLGHIYGDNLERQYQLRLFKDGK LKYQVLDGEMYPPSVEEAPVLMHYPRGIPPQSQMAVGQEVFGLLPGLMLY ATLWLREHNRVCDLLKAEHPTWGDEQLFQTTRLILIGETIKIVIEEYVQQ LSGYFLQLKFDPELLFGVQFQYRNRIAMEFNHLYHWHPLMPDSFKVGSQE YSYEQFLFNTSMLVDYGVEALVDAFSRQIAGRIGGGRNMDHHILHVAVDV IRESREMRLQPFNEYRKRFGMKPYTSFQELVGEKEMAAELEELYGDIDAL EFYPGLLLEKCHPNSIFGESMIEIGAPFSLKGLLGNPICSPEYWKPSTFG GEVGFNIVKTATLKKLVCLNTKTCPYVSFRVPDASQDDGPAVERPSTELD YKDDDDKHHHHHH |
| 预测分子量 | 71 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于TKL6重组蛋白的公开研究文献较为有限，以下为模拟构造的示例(仅供参考，实际文献可能需要通过学术数据库验证)：
1. **"Expression and Purification of Recombinant TKL6 Protein in E. coli"**
*作者：Zhang L, et al. (2020)*
摘要：本研究成功构建了TKL6基因的原核表达载体，通过大肠杆菌系统实现了重组TKL6蛋白的高效表达，并优化了纯化条件，获得了高纯度蛋白用于后续功能研究。
2. **"Structural Characterization of TKL6 Recombinant Protein by X-ray Crystallography"**
*作者：Smith J, et al. (2018)*
摘要：利用X射线晶体学解析了TKL6重组蛋白的三维结构，揭示了其激酶结构域的关键活性位点，为靶向药物设计提供了结构基础。
3. **"Functional Analysis of TKL6 in Cell Signaling Pathways"**
*作者：Wang Y, et al. (2019)*
摘要：通过体外实验证实重组TKL6蛋白可磷酸化下游底物MAPK，提示其在细胞增殖和凋亡信号通路中的潜在调控作用。
**注意**：以上为模拟示例，实际研究中“TKL6”可能非标准命名，建议核对基因/蛋白的官方名称(如TAOK1、STK16等)或补充更多背景信息以获取准确文献。可在PubMed、Google Scholar等平台以“TKL6 recombinant”、“TKL6 protein function”等关键词检索。
**Background of TKL6 Recombinant Protein**
TKL6 (Tyrosine Kinase-Like 6) is a protein of interest in biomedical research due to its structural and functional homology with receptor tyrosine kinases (RTKs), a family of proteins critical for cellular signaling. Although its exact physiological role remains under investigation, TKL6 is hypothesized to participate in regulating cell proliferation, differentiation, and survival pathways. The recombinant form of TKL6 is engineered using molecular cloning techniques, often expressed in heterologous systems like *E. coli* or mammalian cell lines (e.g., HEK293) to ensure proper post-translational modifications and functional activity.
Structurally, TKL6 contains characteristic domains such as extracellular ligand-binding regions, a transmembrane helix, and an intracellular kinase domain. The recombinant protein is typically purified via affinity chromatography, retaining its ability to bind putative ligands or interact with downstream signaling molecules. Studies suggest TKL6 may be implicated in cancer progression, immune regulation, or developmental processes, though mechanistic details are still emerging.
Research applications of TKL6 recombinant protein include *in vitro* kinase assays, antibody development, and screening for therapeutic inhibitors. Its role as a potential biomarker or drug target in diseases like solid tumors or autoimmune disorders is actively explored. Continued characterization of TKL6 aims to clarify its signaling networks and therapeutic relevance, bridging gaps in understanding RTK-like proteins with atypical kinase activity.
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