| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LPHN3 |
| Uniprot No | Q9HAR2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LPHN3重组蛋白研究的3篇代表性文献(注：部分文献信息为简化示例，实际引用需核实原文)：
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1. **文献名称**：*Structural and functional analysis of the adhesion G protein-coupled receptor latrophilin-3*
**作者**：Boucard, A. A., et al.
**摘要**：该研究解析了LPHN3重组蛋白的胞外结构域，揭示了其与配体teneurin的结合位点，并证明其通过钙离子依赖性机制调控突触黏附功能。
2. **文献名称**：*LPHN3 recombinant fragments mediate trans-cellular signaling in neurodevelopmental models*
**作者**：Silva, J. P., & Südhof, T. C.
**摘要**：利用重组LPHN3蛋白片段，验证了其在神经元突触形成中的关键作用，发现其与FLRT3蛋白的相互作用可能影响神经回路发育。
3. **文献名称**：*Expression and purification of functional LPHN3 extracellular domain in mammalian cells*
**作者**：O’Sullivan, M. L., et al.
**摘要**：开发了一种哺乳动物细胞表达系统，成功获得高纯度LPHN3重组胞外域蛋白，并证实其保留天然构象和配体结合活性，为后续药物筛选提供工具。
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(注：以上为基于领域研究的概括性示例，实际文献需通过PubMed或专业数据库检索确认。)
LPHN3 (Latrophilin-3), encoded by the ADGRL3 gene, is a member of the adhesion G protein-coupled receptor (aGPCR) family. It plays a critical role in synaptic adhesion, cell signaling, and neuronal connectivity. Structurally, LPHN3 contains a large extracellular domain with adhesion motifs (e.g., lectin, olfactomedin-like, and hormone-binding domains), a seven-transmembrane region, and an intracellular PDZ-binding motif. It interacts with ligands like teneurins and FLRTs, mediating trans-cellular signaling during brain development.
LPHN3 gained prominence due to its association with neuropsychiatric disorders. Genome-wide studies linked ADGRL3 variants to attention-deficit/hyperactivity disorder (ADHD), impulsivity, and addiction behaviors. Its role in dopamine-regulated pathways and synaptic plasticity underscores its relevance to neurotransmission dysregulation. Animal models with Lphn3 knockdown exhibit hyperactivity and altered reward processing, mirroring ADHD phenotypes.
Recombinant LPHN3 protein is engineered to study its molecular interactions and signaling mechanisms. Typically produced in mammalian or insect cell systems, it retains post-translational modifications critical for ligand binding and receptor activation. Researchers use it to map binding interfaces, characterize structural domains, and screen for modulators. For example, studies employing recombinant extracellular domains revealed how LPHN3-teneurin complexes regulate synapse formation.
The protein also aids drug discovery, particularly for ADHD therapeutics targeting aGPCRs. Its recombinant form enables high-throughput assays to identify small molecules or antibodies that modulate receptor activity. However, challenges remain in resolving full-length structures and understanding context-dependent signaling. Ongoing research leverages recombinant LPHN3 to decode its dual roles as both adhesion molecule and GPCR, bridging neurodevelopment and behavioral disorders.
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