| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LPHN2 |
| Uniprot No | O95490 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LPHN2重组蛋白的3篇参考文献示例(注：文献信息为虚拟示例，仅作格式参考)：
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1. **标题**: *Recombinant LPHN2 extracellular domain expression and its role in synaptic adhesion*
**作者**: Smith J, et al.
**摘要**: 本研究成功在大肠杆菌系统中表达了LPHN2的胞外域重组蛋白，并验证其与配体teneurin的结合能力，揭示了其在神经元突触粘附中的潜在作用。
2. **标题**: *Structural analysis of LPHN2 using cryo-EM reveals activation mechanisms*
**作者**: Lee S, et al.
**摘要**: 通过昆虫细胞表达系统获得全长LPHN2重组蛋白，利用冷冻电镜解析其三维结构，发现其胞内区构象变化与G蛋白信号激活的关联。
3. **标题**: *LPHN2 recombinant protein promotes cancer cell migration via GPCR signaling*
**作者**: Wang Y, et al.
**摘要**: 在哺乳动物HEK293细胞中表达并纯化LPHN2重组蛋白，功能实验表明其通过激活下游RhoA通路增强肿瘤细胞迁移能力。
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**注意**：以上文献为假设性描述，实际研究中请通过PubMed或Web of Science检索真实文献(关键词：LPHN2/Latrophilin-2 + recombinant/protein expression/structure)。如需具体文献协助，请提供更多研究背景。
**Background of LPHN2 Recombinant Protein**
Latrophilin-2 (LPHN2), a member of the adhesion G protein-coupled receptor (GPCR) family, plays critical roles in cell adhesion, synaptic signaling, and intercellular communication. It is encoded by the *ADGRL2* gene and is part of a three-member latrophilin subfamily (LPHN1-3). Structurally, LPHN2 features a large extracellular region containing lectin, olfactomedin, and hormone receptor domains, a transmembrane domain, and a cytoplasmic tail with a PDZ-binding motif. These domains enable interactions with diverse ligands, including teneurins and fibronectin leucine-rich transmembrane proteins (FLRTs), facilitating cell-matrix adhesion and synaptic organization.
In the central nervous system, LPHN2 is enriched in postsynaptic membranes and regulates synapse formation, plasticity, and neurotransmitter release. It also influences neurodevelopment by modulating axon guidance and neuronal migration. Beyond the brain, LPHN2 is expressed in cardiovascular, endocrine, and immune tissues, suggesting roles in angiogenesis, hormone secretion, and inflammation. Dysregulation of LPHN2 is linked to neuropsychiatric disorders (e.g., ADHD, autism), cancer metastasis, and cardiovascular diseases.
Recombinant LPHN2 protein, typically produced in mammalian or insect cell systems, retains functional domains and post-translational modifications. It is engineered with tags (e.g., Fc or His tags) for purification and detection. Researchers use this protein to study receptor-ligand interactions, signaling pathways, and disease mechanisms. For example, it aids in screening small molecules targeting LPHN2 for therapeutic development. Its application extends to structural studies (e.g., cryo-EM) to resolve activation mechanisms of adhesion GPCRs.
Overall, LPHN2 recombinant protein serves as a vital tool for deciphering the biology of adhesion GPCRs and exploring their therapeutic potential in diverse pathologies.
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