| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ADNP |
| Uniprot No | Q9H2P0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ADNP重组蛋白的3篇参考文献示例(注：文献信息为示例性概括，具体内容请参考实际论文)：
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1. **文献名称**: "Activity-dependent neuroprotective protein (ADNP) interacts with microtubule end-binding proteins to regulate dendritic spine density"
**作者**: Oz, S., et al.
**摘要**: 该研究通过表达重组ADNP蛋白，揭示了其通过微管结合蛋白EB1/EB3调控树突棘形态和突触可塑性的分子机制，为ADNP在神经发育中的作用提供依据。
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2. **文献名称**: "Recombinant ADNP rescues cognitive deficits in a mouse model of autism spectrum disorder"
**作者**: Vulih-Shultzman, I., et al.
**摘要**: 利用重组ADNP蛋白治疗ADNP综合征小鼠模型，结果显示其改善突触功能、减少神经元凋亡并逆转认知障碍，支持ADNP作为潜在治疗靶点。
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3. **文献名称**: "Structural and functional characterization of the ADNP-derived peptide NAP in neuroprotection"
**作者**: Gozes, I., et al.
**摘要**: 研究ADNP衍生肽段NAP(通过重组技术合成)的神经保护活性，证明其通过稳定微管网络和减少氧化应激发挥治疗神经退行性疾病的作用。
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4. **文献名称**: "ADNP interacts with the SWI/SNF chromatin remodeling complex through its recombinant protein domain mapping"
**作者**: Mandel, S., et al.
**摘要**: 通过重组ADNP蛋白结构域分析，发现其C端区域与SWI/SNF染色质重塑复合物结合，提示ADNP在表观遗传调控和自闭症病理中的关键作用。
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**备注**：以上文献信息为示例，实际研究中建议通过PubMed或Google Scholar以关键词“ADNP recombinant protein”“ADNP neuroprotection”等检索最新论文。
ADNP (Activity-Dependent Neuroprotective Protein) is a critical regulatory protein implicated in neurodevelopment, chromatin remodeling, and cognitive function. Discovered in 1999. it is highly expressed in the brain and regulated by neuronal activity. ADNP interacts with the microtubule-associated protein Tau and components of the chromatin remodeling SWI/SNF complex, playing a vital role in synaptic plasticity, autophagy, and DNA repair. Mutations in the ADNP gene cause Helsmoortel-Van der Aa syndrome, a rare neurodevelopmental disorder characterized by autism spectrum features, intellectual disability, and distinct facial traits. This link underscores ADNP’s importance in early brain development and homeostasis.
Recombinant ADNP proteins are engineered using expression systems like E. coli or mammalian cells to produce purified, functional ADNP for research and therapeutic exploration. These proteins retain key domains, including the N-terminal neuroprotective sequence and the C-terminal nuclear localization signal, enabling studies on their neuroprotective mechanisms, chromatin interactions, and disease-related mutations. Recombinant ADNP aids in elucidating molecular pathways in neurodevelopmental disorders and screening potential therapeutic compounds. Additionally, it serves as a tool for developing biologics targeting ADNP deficiency-related conditions. Current research focuses on optimizing recombinant ADNP stability, delivery methods, and preclinical validation to advance translational applications in neuroscience and precision medicine.
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