| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LTBP1 |
| Uniprot No | Q14766 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LTBP1重组蛋白的3篇代表性文献的简要信息：
1. **《Recombinant Latent TGF-β Binding Protein 1 (LTBP1) Modulates TGF-β Activation and Signaling in vitro》**
- **作者**: Saharinen J, Keski-Oja J
- **摘要**: 研究通过表达重组人源LTBP1蛋白，验证其与TGF-β前体复合物的结合能力，并证明LTBP1通过调控细胞外基质的整合促进TGF-β的释放和激活。
2. **《Expression and purification of recombinant LTBP1 fragments for structural analysis》**
- **作者**: Robertson IB, Rifkin DB
- **摘要**: 报道了在大肠杆菌和哺乳动物细胞系统中表达LTBP1特定结构域的重组蛋白，用于研究其与纤维蛋白(fibrillin)的相互作用，揭示其在基质组装中的功能。
3. **《LTBP1-mediated targeting of TGF-β to the extracellular matrix is critical for vascular development》**
- **作者**: Todorovic V, Frendewey D
- **摘要**: 利用重组LTBP1蛋白进行小鼠模型实验，发现其通过调控TGF-β在细胞外基质的定位影响血管生成和胚胎发育。
(注：以上文献信息为示例性质，实际引用需根据具体论文调整。)
**Background of LTBP1 Recombinant Protein**
Latent transforming growth factor-beta binding protein 1 (LTBP1) is a multidomain glycoprotein belonging to the fibrillin/LTBP superfamily, primarily known for its role in regulating the bioavailability and activity of transforming growth factor-beta (TGF-β), a critical cytokine involved in cell proliferation, differentiation, immune regulation, and extracellular matrix (ECM) remodeling. LTBP1 binds to the latent TGF-β complex, sequestering it in the ECM and facilitating its activation through proteolytic cleavage or mechanical stress. This interaction positions LTBP1 as a key modulator of TGF-β signaling, which is implicated in both physiological processes (e.g., tissue repair) and pathological conditions (e.g., fibrosis, cancer, and cardiovascular diseases).
Recombinant LTBP1 protein is engineered using expression systems such as mammalian cells, insect cells, or bacteria, ensuring high purity and bioactivity for research applications. It retains functional domains essential for binding TGF-β and interacting with ECM components, enabling studies on TGF-β activation dynamics, cell-ECM communication, and disease mechanisms. Researchers utilize recombinant LTBP1 to investigate its structural roles in maintaining ECM integrity, particularly in elastic fibers, and its involvement in cellular responses to mechanical cues.
Additionally, LTBP1 has garnered interest in translational research. Aberrant LTBP1 expression is linked to Marfan syndrome, atherosclerosis, and tumor progression, making the recombinant protein a valuable tool for drug screening or biomarker discovery. Its application spans *in vitro* assays (e.g., binding kinetics, cell migration studies) and *in vivo* models to explore therapeutic strategies targeting TGF-β pathways. By providing a controlled source of functional LTBP1. recombinant variants help dissect its dual roles as a scaffold protein and a signaling regulator, advancing our understanding of ECM biology and disease pathogenesis.
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