| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NUP88 |
| Uniprot No | Q99567 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-741aa |
| 氨基酸序列 | MAAAEGPVGD GELWQTWLPN HVVFLRLREG LKNQSPTEAE KPASSSLPSS PPPQLLTRNV VFGLGGELFL WDGEDSSFLV VRLRGPSGGG EEPALSQYQR LLCINPPLFE IYQVLLSPTQ HHVALIGIKG LMVLELPKRW GKNSEFEGGK STVNCSTTPV AERFFTSSTS LTLKHAAWYP SEILDPHVVL LTSDNVIRIY SLREPQTPTN VIILSEAEEE SLVLNKGRAY TASLGETAVA FDFGPLAAVP KTLFGQNGKD EVVAYPLYIL YENGETFLTY ISLLHSPGNI GKLLGPLPMH PAAEDNYGYD ACAVLCLPCV PNILVIATES GMLYHCVVLE GEEEDDHTSE KSWDSRIDLI PSLYVFECVE LELALKLASG EDDPFDSDFS CPVKLHRDPK CPSRYHCTHE AGVHSVGLTW IHKLHKFLGS DEEDKDSLQE LSTEQKCFVE HILCTKPLPC RQPAPIRGFW IVPDILGPTM ICITSTYECL IWPLLSTVHP ASPPLLCTRE DVEVAESPLR VLAETPDSFE KHIRSILQRS VANPAFLKAS EKDIAPPPEE CLQLLSRATQ VFREQYILKQ DLAKEEIQRR VKLLCDQKKK QLEDLSYCRE ERKSLREMAE RLADKYEEAK EKQEDIMNRM KKLLHSFHSE LPVLSDSERD MKKELQLIPD QLRHLGNAIK QVTMKKDYQQ QKMEKVLSLP KPTIILSAYQ RKCIQSILKE EGEHIREMVK QINDIRNHVN F |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NUP88重组蛋白的3篇参考文献(内容基于公开研究整理，非真实文献，仅作示例)：
1. **文献名称**：*NUP88重组蛋白的克隆表达及其在核转运机制中的功能研究*
**作者**：Smith A, et al.
**摘要**：研究通过大肠杆菌系统表达并纯化NUP88重组蛋白，验证其与核孔复合体其他组分(如NUP214)的相互作用，揭示其在mRNA出核转运中的调控作用。
2. **文献名称**：*NUP88过表达与卵巢癌转移的相关性分析*
**作者**：Zhang Y, et al.
**摘要**：利用重组NUP88蛋白进行体外实验，发现其过表达通过激活Wnt/β-catenin通路促进肿瘤细胞侵袭，提示NUP88可能作为癌症治疗靶点。
3. **文献名称**：*NUP88与病毒蛋白相互作用调控HIV-1复制的研究*
**作者**：Lee C, et al.
**摘要**：通过重组NUP88蛋白结合实验，证实其与HIV-1 Rev蛋白结合，调控病毒RNA的核质运输，为抗病毒药物开发提供新方向。
4. **文献名称**：*NUP88重组蛋白的晶体结构解析及其功能域鉴定*
**作者**：Tanaka K, et al.
**摘要**：采用X射线衍射技术解析NUP88重组蛋白的晶体结构，鉴定出关键功能域，为研究核孔复合体组装机制提供结构基础。
(注：以上文献为示例，实际引用时需核实真实数据库如PubMed、ScienceDirect等。)
NUP88 (Nucleoporin 88) is a key component of the nuclear pore complex (NPC), a large protein assembly embedded in the nuclear envelope that regulates nucleocytoplasmic transport of molecules. It belongs to the nucleoporin family and interacts with other nucleoporins, such as NUP98. to form a dynamic scaffold critical for maintaining NPC architecture and function. NUP88 is involved in selective transport mechanisms, cell cycle regulation, and mitotic processes, with emerging roles in transcriptional control and chromatin organization.
Recombinant NUP88 refers to the protein produced artificially using genetic engineering techniques, often in bacterial or eukaryotic expression systems. Its recombinant form enables detailed biochemical and structural studies to dissect molecular interactions within the NPC, particularly its role in anchoring transport factors or mediating protein-protein interactions. Research has linked NUP88 overexpression to various cancers, including leukemia and solid tumors, where it may promote oncogenic signaling by stabilizing cancer-related proteins or disrupting nuclear transport. Additionally, NUP88 fusion proteins, resulting from chromosomal translocations, are implicated in cellular transformation.
The availability of recombinant NUP88 facilitates investigations into its pathological mechanisms, serving as a tool for drug screening, antibody development, and diagnostic assays. Studies also explore its interplay with viral pathogens, as some viruses exploit NPC components for nuclear entry. Despite progress, questions remain about its precise regulatory roles and post-translational modifications. Recombinant NUP88 thus represents a vital resource for advancing nuclear transport biology, cancer research, and therapeutic targeting of NPC-associated diseases.
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