| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HDAC4 |
| Uniprot No | P56524 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 101-1084aa |
| 氨基酸序列 | SRQHEAQLHEHIKQQQEMLAMKHQQELLEHQRKLERHRQEQELEKQHREQ KLQQLKNKEKGKESAVASTEVKMKLQEFVLNKKKALAHRNLNHCISSDPR YWYGKTQHSSLDQSSPPQSGVSTSYNHPVLGMYDAKDDFPLRKTASEPNL KLRSRLKQKVAERRSSPLLRRKDGPVVTALKKRPLDVTDSACSSAPGSGP SSPNNSSGSVSAENGIAPAVPSIPAETSLAHRLVAREGSAAPLPLYTSPS LPNITLGLPATGPSAGTAGQQDAERLTLPALQQRLSLFPGTHLTPYLSTS PLERDGGAAHSPLLQHMVLLEQPPAQAPLVTGLGALPLHAQSLVGADRVS PSIHKLRQHRPLGRTQSAPLPQNAQALQHLVIQQQHQQFLEKHKQQFQQQ QLQMNKIIPKPSEPARQPESHPEETEEELREHQALLDEPYLDRLPGQKEA HAQAGVQVKQEPIESDEEEAEPPREVEPGQRQPSEQELLFRQQALLLEQQ RIHQLRNYQASMEAAGIPVSFGGHRPLSRAQSSPASATFPVSVQEPPTKP RFTTGLVYDTLMLKHQCTCGSSSSHPEHAGRIQSIWSRLQETGLRGKCEC IRGRKATLEELQTVHSEAHTLLYGTNPLNRQKLDSKKLLGSLASVFVRLP CGGVGVDSDTIWNEVHSAGAARLAVGCVVELVFKVATGELKNGFAVVRPP GHHAEESTPMGFCYFNSVAVAAKLLQQRLSVSKILIVDWDVHHGNGTQQA FYSDPSVLYMSLHRYDDGNFFPGSGAPDEVGTGPGVGFNVNMAFTGGLDP PMGDAEYLAAFRTVVMPIASEFAPDVVLVSSGFDAVEGHPTPLGGYNLSA RCFGYLTKQLMGLAGGRIVLALEGGHDLTAICDASEACVSALLGNELDPL PEKVLQQRPNANAVRSMEKVMEIHSKYWRCLQRTTSTAGRSLIEAQTCEN EEAETVTAMASLSVGVKPAEKRPDEEPMEEEPPL |
| 预测分子量 | 155kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HDAC4重组蛋白的3篇参考文献及其简要摘要：
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1. **文献名称**：*Crystal Structure of Human Histone Deacetylase 4 (HDAC4) in Complex with a Hydroxamate Inhibitor*
**作者**：Bottomley, M.J. et al.
**摘要**：该研究解析了重组人源HDAC4蛋白的晶体结构，揭示了其催化结构域与羟肟酸类抑制剂的结合模式，阐明了HDAC4底物识别的分子机制，为靶向HDAC4的药物设计提供了结构基础。
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2. **文献名称**：*Regulation of Muscle Differentiation by HDAC4 through Interaction with MEF2 Transcription Factors*
**作者**：Miska, E.A. et al.
**摘要**：通过重组HDAC4蛋白与转录因子MEF2的体外结合实验，证明HDAC4通过去乙酰化作用抑制MEF2活性，调控肌肉分化进程，揭示了其在发育中的关键调控功能。
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3. **文献名称**：*Phosphorylation-dependent Interaction of HDAC4 with 14-3-3 Proteins*
**作者**：Wang, A.H. & Yang, X.J.
**摘要**：研究利用重组HDAC4蛋白证明其磷酸化后与14-3-3蛋白结合，导致HDAC4核质穿梭受阻，进而影响下游基因表达，阐明了翻译后修饰对其亚细胞定位的调控机制。
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4. **文献名称**：*Recombinant HDAC4 Expression and Enzymatic Characterization in Neurodegenerative Models*
**作者**：Sando, R. et al.
**摘要**：该研究在大肠杆菌中高效表达并纯化重组HDAC4蛋白，分析其酶动力学特性，并验证其在神经元模型中对tau蛋白乙酰化的调控作用，提示HDAC4在神经退行性疾病中的潜在治疗价值。
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这些文献涵盖了HDAC4重组蛋白的结构解析、相互作用机制及功能研究，为理解其生物学作用提供了多角度参考。
**Background of HDAC4 Recombinant Protein**
Histone deacetylase 4 (HDAC4) is a member of the class IIa HDAC family, which regulates gene expression by modifying chromatin structure through the removal of acetyl groups from histone lysine residues. Unlike class I HDACs, class IIa HDACs (HDAC4. 5. 7. 9) exhibit tissue-specific expression and shuttle between the nucleus and cytoplasm, acting as transcriptional repressors in coordination with co-regulatory complexes. HDAC4 is highly expressed in tissues such as heart, skeletal muscle, and brain, playing critical roles in development, differentiation, and cellular stress responses.
Structurally, HDAC4 contains an N-terminal regulatory domain that mediates protein-protein interactions and a C-terminal catalytic domain. However, its deacetylase activity is intrinsically weak, requiring interaction with corepressors like HDAC3 for full functionality. HDAC4 primarily regulates non-histone substrates, influencing pathways such as MEF2-mediated transcription, muscle atrophy, and neuronal survival. Dysregulation of HDAC4 is linked to diseases including cancers, neurodegenerative disorders, and cardiovascular conditions, making it a therapeutic target.
Recombinant HDAC4 protein is produced in heterologous systems (e.g., *E. coli* or mammalian cells*) to study its biochemical properties, enzymatic activity, and interactions. Purified HDAC4 is often fused with tags (e.g., GST, His) for efficient isolation and detection. It serves as a vital tool for *in vitro* assays, inhibitor screening, and structural studies to elucidate mechanisms of deacetylation and develop targeted therapies. Research using recombinant HDAC4 has advanced understanding of epigenetic regulation and its role in disease pathogenesis, highlighting its potential in drug discovery and molecular biology.
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