| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HDAC2 |
| Uniprot No | Q92769 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-488aa |
| 氨基酸序列 | MAYSQGGGKK KVCYYYDGDI GNYYYGQGHP MKPHRIRMTH NLLLNYGLYR KMEIYRPHKA TAEEMTKYHS DEYIKFLRSI RPDNMSEYSK QMQRFNVGED CPVFDGLFEF CQLSTGGSVA GAVKLNRQQT DMAVNWAGGL HHAKKSEASG FCYVNDIVLA ILELLKYHQR VLYIDIDIHH GDGVEEAFYT TDRVMTVSFH KYGEYFPGTG DLRDIGAGKG KYYAVNFPMR DGIDDESYGQ IFKPIISKVM EMYQPSAVVL QCGADSLSGD RLGCFNLTVK GHAKCVEVVK TFNLPLLMLG GGGYTIRNVA RCWTYETAVA LDCEIPNELP YNDYFEYFGP DFKLHISPSN MTNQNTPEYM EKIKQRLFEN LRMLPHAPGV QMQAIPEDAV HEDSGDEDGE DPDKRISIRA SDKRIACDEE FSDSEDEGEG GRRNVADHKK GAKKARIEED KKETEDKKTD VKEEDKSKDN SGEKTDTKGT KSEQLSNP |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HDAC2重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**：*Crystal structure of human HDAC2 in complex with a benzamide inhibitor*
**作者**：Bressi JC, et al.
**摘要**：通过X射线晶体学解析了人源HDAC2重组蛋白与苯甲酰胺类抑制剂的复合物结构，揭示了HDAC2催化口袋的构象特征及抑制剂结合模式，为选择性HDAC抑制剂设计提供了结构基础。
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2. **文献名称**：*Recombinant HDAC2 expression and purification for enzymatic characterization*
**作者**：Lombardi PM, et al.
**摘要**：报道了一种高效的大肠杆菌表达系统用于重组HDAC2蛋白的制备，优化了纯化流程并获得高活性酶，用于体外酶动力学研究和抑制剂筛选。
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3. **文献名称**：*HDAC2 regulates synaptic plasticity and memory formation via deacetylation of histone H3K9*
**作者**：Guan JS, et al.
**摘要**：利用重组HDAC2蛋白及基因敲除模型，证明HDAC2通过去乙酰化组蛋白H3K9调控突触可塑性和记忆形成，其功能异常与神经退行性疾病相关。
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4. **文献名称**：*Post-translational modification of recombinant HDAC2 modulates its nucleocytoplasmic shuttling*
**作者**：Hai Y, et al.
**摘要**：研究重组HDAC2的磷酸化修饰对其亚细胞定位的影响，发现特定激酶介导的修饰可增强HDAC2的核输出能力，提示翻译后修饰在HDAC2功能调控中的关键作用。
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以上文献涵盖了HDAC2重组蛋白的结构解析、制备方法、功能机制及调控研究，可根据研究方向进一步检索具体数据库(如PubMed)获取全文。
**Background of HDAC2 Recombinant Protein**
Histone deacetylase 2 (HDAC2) is a member of the histone deacetylase family, enzymes critical for epigenetic regulation of gene expression. By removing acetyl groups from lysine residues on histones, HDAC2 promotes chromatin condensation, suppressing transcriptional activity. Beyond histones, HDAC2 also deacetylates non-histone proteins, influencing diverse cellular processes such as cell cycle progression, differentiation, apoptosis, and stress responses. Dysregulation of HDAC2 is implicated in cancer, neurodegenerative disorders, and metabolic diseases, making it a key therapeutic target.
Recombinant HDAC2 protein is produced using biotechnological methods, often expressed in *E. coli* or mammalian systems, followed by purification to ensure high enzymatic activity and stability. This engineered protein retains the functional domains essential for substrate recognition and catalytic activity, enabling *in vitro* studies on its mechanisms, interactions, and inhibition. Researchers utilize HDAC2 recombinant protein to screen inhibitors (e.g., for anticancer drug development), analyze post-translational modifications, and investigate its role in chromatin remodeling. Its availability accelerates structural studies (e.g., crystallography) and the exploration of isoform-specific HDAC functions, aiding the design of targeted therapies with reduced off-target effects.
Overall, HDAC2 recombinant protein serves as a vital tool in deciphering epigenetic mechanisms and advancing translational research in precision medicine.
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