| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HIST2H2AC |
| Uniprot No | Q16777 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-129aa |
| 氨基酸序列 | SGRGKQGGK ARAKAKSRSS RAGLQFPVGR VHRLLRKGNY AERVGAGAPV YMAAVLEYLT AEILELAGNA ARDNKKTRII PRHLQLAIRN DEELNKLLGK VTIAQGGVLP NIQAVLLPKK TESHKAKSK |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HIST2H2AC重组蛋白的3篇示例文献(注：文献为示例虚构，实际引用需查询真实数据库)：
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1. **文献名称**：Recombinant Expression and Purification of Human HIST2H2AC for Epigenetic Studies
**作者**：Zhang L, et al.
**摘要**：该研究成功在大肠杆菌中表达并纯化了重组HIST2H2AC蛋白，优化了表达条件以提高溶解度。纯化后的蛋白通过质谱和圆二色光谱验证结构完整性，并用于体外核小体组装实验，探讨其与DNA结合的动力学特性。
2. **文献名称**：Post-Translational Modifications of Recombinant HIST2H2AC Regulate Chromatin Dynamics
**作者**：Kim S, Patel R
**摘要**：作者利用昆虫细胞系统表达重组HIST2H2AC，研究其泛素化修饰对染色质重塑的影响。实验表明，特定位点的泛素化调控了HIST2H2AC与其他组蛋白(如H2B)的相互作用，影响基因沉默通路。
3. **文献名称**：HIST2H2AC Recombinant Protein as a Biomarker in Cancer Cell Lines
**作者**：Gupta M, et al.
**摘要**：通过哺乳动物细胞表达重组HIST2H2AC，发现其在乳腺癌细胞中异常高表达。该蛋白与癌基因产物相互作用，可能通过表观遗传失调促进肿瘤发生，提示其作为潜在治疗靶点。
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如需真实文献，建议通过PubMed或Google Scholar以关键词“HIST2H2AC recombinant”检索，并筛选涉及重组表达、功能或应用的论文。
The HIST2H2AC gene encodes a member of the histone H2A family, a core component of nucleosomes that organize eukaryotic DNA into chromatin. As part of the H2A histone cluster on chromosome 1q21.2. HIST2H2AC contributes to the octameric protein complex (two each of H2A, H2B, H3. and H4) around which DNA is wrapped, forming the basic structural unit of chromatin. Histone H2A proteins, including HIST2H2AC, play critical roles in maintaining chromatin stability, regulating gene expression, and facilitating DNA repair and replication. Unlike canonical H2A histones, certain H2A variants (e.g., H2A.X, macroH2A) exhibit specialized functions, but HIST2H2AC is considered a replication-dependent "canonical" H2A isoform predominantly expressed during the S phase of the cell cycle.
Recombinant HIST2H2AC protein is engineered for experimental studies, often produced in bacterial (e.g., *E. coli*) or eukaryotic expression systems to ensure proper folding and post-translational modification mimicry. Its recombinant form enables researchers to study nucleosome assembly/disassembly dynamics, histone-DNA interactions, and epigenetic regulation. It is particularly valuable in chromatin remodeling studies, in vitro reconstitution of nucleosomes, and investigations into histone modification impacts (e.g., acetylation, ubiquitination) on gene silencing or activation. Dysregulation of HIST2H2AC has been linked to cancers and developmental disorders, making its recombinant version a tool for probing disease mechanisms. Additionally, it aids in developing histone-based therapies and understanding cellular responses to DNA damage. The protein’s accessibility through recombinant technology has significantly advanced epigenetics and molecular biology research.
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