| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HIST1H2AC |
| Uniprot No | Q93077 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-130aa |
| 氨基酸序列 | SGRGKQGGK ARAKAKSRSS RAGLQFPVGR VHRLLRKGNY AERVGAGAPV YLAAVLEYLT AEILELAGNA ARDNKKTRII PRHLQLAIRN DEELNKLLGR VTIAQGGVLP NIQAVLLPKK TESHHKAKGK |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HIST1H2AC重组蛋白的3篇假设性参考文献(基于研究领域常见主题的合理推测，非真实文献)：
1. **"Recombinant HIST1H2AC facilitates nucleosome assembly and chromatin remodeling in vitro"**
- **作者**: Zhang, Y. et al.
- **摘要**: 本研究通过在大肠杆菌中表达并纯化HIST1H2AC重组蛋白，验证其与组蛋白H2B、H3/H4复合物的结合能力，证明其在体外核小体组装和染色质结构动态调控中的关键作用。
2. **"Post-translational modifications of HIST1H2AC regulate DNA damage response"**
- **作者**: Lee, S. & Kim, J.
- **摘要**: 利用重组HIST1H2AC蛋白分析其磷酸化和泛素化修饰位点，揭示这些修饰在DNA损伤修复过程中通过影响染色质松解促进修复因子招募的分子机制。
3. **"HIST1H2AC overexpression promotes tumorigenesis in breast cancer models"**
- **作者**: Gupta, R. et al.
- **摘要**: 通过体外表达HIST1H2AC重组蛋白，研究其在乳腺癌细胞中的功能，发现其异常表达通过激活促癌基因转录和抑制抑癌基因，加速肿瘤细胞增殖。
4. **"Structural insights into HIST1H2AC-DNA interactions by cryo-EM"**
- **作者**: Müller, C.W. et al.
- **摘要**: 结合重组HIST1H2AC蛋白和DNA进行冷冻电镜分析，解析其与DNA结合的精确构象变化，为组蛋白变体在基因特异性调控中的作用提供结构基础。
(注：以上为模拟文献，实际文献需通过PubMed或Google Scholar检索。)
The HIST1H2AC protein is a member of the histone H2A family, which plays a fundamental role in organizing eukaryotic DNA into chromatin. As a core component of nucleosomes, H2A histones form an octamer with other histones (H2B, H3. and H4), around which DNA is wrapped to maintain genome stability and regulate gene expression. HIST1H2AC, encoded by the HIST1H2AC gene located on chromosome 6 (6p22.2), is a replication-dependent histone involved in chromatin assembly during DNA replication and repair. Its recombinant form is produced using expression systems like *E. coli* or mammalian cells, enabling precise study of its structural and functional properties.
Recombinant HIST1H2AC is widely used to investigate chromatin dynamics, epigenetic modifications, and DNA-protein interactions. It serves as a tool for studying post-translational modifications (e.g., acetylation, ubiquitination) that influence gene regulation, cell cycle progression, and responses to DNA damage. Researchers employ it in *in vitro* assays, such as nucleosome reconstitution, to explore how histone variants or mutations alter chromatin structure and function. Abnormalities in HIST1H2AC expression or modification have been linked to cancers and developmental disorders, making it a focus in disease mechanism studies. Additionally, recombinant HIST1H2AC supports drug discovery efforts targeting epigenetic regulators. Its availability in pure, functional form accelerates research into chromatin biology and therapeutic interventions.
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