| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HRH4 |
| Uniprot No | Q9H3N8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-390aa |
| 氨基酸序列 | MPDTNSTINLSLSTRVTLAFFMSLVAFAIMLGNALVILAFVVDKNLRHRSSYFFLNLAISDFFVGVISIPLYIPHTLFEWDFGKEICVFWLTTDYLLCTASVYNIVLISYDRYLSVSNAVSYRTQHTGVLKIVTLMVAVWVLAFLVNGPMILVSESWKDEGSECEPGFFSEWYILAITSFLEFVIPVILVAYFNMNIYWSLWKRDHLSRCQSHPGLTAVSSNICGHSFRGRLSSRRSLSASTEVPASFHSERQRRKSSLMFSSRTKMNSNTIASKMGSFSQSDSVALHQREHVELLRARRLAKSLAILLGVFAVCWAPYSLFTIVLSFYSSATGPKSVWYRIAFWLQWFNSFVNPLLYPLCHKRFQKAFLKIFCIKKQPLPSQHSRSVSS |
| 预测分子量 | 44,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HRH4重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**: *Expression and Functional Characterization of Recombinant Human Histamine H4 Receptor in Insect Cells*
**作者**: Nakamura, T., et al.
**摘要**: 本研究利用杆状病毒-昆虫细胞系统成功表达并纯化重组人源HRH4蛋白，通过G蛋白偶联信号通路分析，证实其与组胺及特异性激动剂(如JNJ 7777120)结合后激活下游信号，为HRH4的体外功能研究提供模型。
2. **文献名称**: *Crystal Structure of the Human Histamine H4 Receptor Reveals Ligand Binding Mode*
**作者**: Shimamura, T., et al.
**摘要**: 通过X射线晶体学首次解析HRH4重组蛋白的三维结构，揭示其与拮抗剂结合的活性位点特征，阐明了HRH4与其他组胺受体的结构差异，为靶向药物设计提供结构基础。
3. **文献名称**: *Pharmacological Profiling of Recombinant HRH4 in Drug Discovery Platforms*
**作者**: Thurmond, R.L., et al.
**摘要**: 在哺乳动物细胞(HEK293)中表达重组HRH4.开发高通量筛选平台评估小分子配体的亲和力与选择性，发现新型HRH4拮抗剂在炎症性疾病中的潜在治疗价值。
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**备注**：以上文献信息为示例，实际引用需根据具体研究检索PubMed或SciFinder等数据库获取原文。建议补充检索关键词：*histamine H4 receptor, recombinant expression, structural analysis, drug discovery*。
**Background of HRH4 Recombinant Protein**
The histamine receptor H4 (HRH4), a member of the G-protein-coupled receptor (GPCR) family, was identified in 2000. later than other histamine receptors (H1-H3). It is primarily expressed in immune-related tissues, including bone marrow, spleen, and intestinal cells, and plays a critical role in mediating inflammatory and immune responses. HRH4 activation by histamine triggers intracellular signaling pathways, such as calcium mobilization and MAPK activation, influencing chemotaxis, cytokine release, and immune cell modulation.
Recombinant HRH4 protein, produced via genetic engineering in heterologous systems (e.g., mammalian or insect cells), enables in vitro studies of receptor-ligand interactions, drug screening, and mechanistic research. Its structure includes seven transmembrane domains and ligand-binding regions, making it a target for therapeutic agents aimed at inflammatory diseases, allergies, and autoimmune disorders. Challenges in HRH4 recombinant production include low expression levels and instability, often addressed through codon optimization or fusion tags.
Research highlights HRH4's dual pro- and anti-inflammatory roles, depending on cellular context, underscoring its therapeutic potential. Recombinant HRH4 tools continue to advance drug discovery and our understanding of histamine signaling beyond classical allergic responses.
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