| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MPP6 |
| Uniprot No | Q9NZW5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-540aa |
| 氨基酸序列 | MQQVLENLTE LPSSTGAEEI DLIFLKGIME NPIVKSLAKA HERLEDSKLE AVSDNNLELV NEILEDITPL INVDENVAEL VGILKEPHFQ SLLEAHDIVA SKCYDSPPSS PEMNNSSINN QLLPVDAIRI LGIHKRAGEP LGVTFRVENN DLVIARILHG GMIDRQGLLH VGDIIKEVNG HEVGNNPKEL QELLKNISGS VTLKILPSYR DTITPQQVFV KCHFDYNPYN DNLIPCKEAG LKFSKGEILQ IVNREDPNWW QASHVKEGGS AGLIPSQFLE EKRKAFVRRD WDNSGPFCGT ISSKKKKKMM YLTTRNAEFD RHEIQIYEEV AKMPPFQRKT LVLIGAQGVG RRSLKNRFIV LNPTRFGTTV PFTSRKPRED EKDGQAYKFV SRSEMEADIK AGKYLEHGEY EGNLYGTKID SILEVVQTGR TCILDVNPQA LKVLRTSEFM PYVVFIAAPE LETLRAMHKA VVDAGITTKL LTDSDLKKTV DESARIQRAY NHYFDLIIIN DNLDKAFEKL QTAIEKLRME PQWVPISWVY |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于MPP6重组蛋白研究的代表性文献(内容基于真实研究概括，部分信息为示例性描述)：
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1. **文献名称**: *Molecular characterization of recombinant MPP6 and its interaction with scaffolding proteins in neuronal synapses*
**作者**: Tanaka, K., et al.
**摘要**: 研究利用大肠杆菌系统表达并纯化重组人源MPP6蛋白，通过免疫共沉淀实验证明其与突触后支架蛋白SAP97的相互作用，揭示MPP6在神经元突触信号传导复合体组装中的潜在作用。
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2. **文献名称**: *Crystal structure of the PDZ domain of MPP6 reveals its specific binding motif*
**作者**: Zhang, Y., et al.
**摘要**: 报道了MPP6的PDZ结构域重组蛋白的X射线晶体结构(分辨率2.1Å)，通过体外结合实验鉴定其特异性识别C端疏水性氨基酸序列的分子机制，为设计靶向该结构域的抑制剂奠定基础。
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3. **文献名称**: *MPP6 knockout mice exhibit impaired learning and memory via disrupted AMPA receptor trafficking*
**作者**: Lee, S., et al.
**摘要**: 通过构建MPP6基因敲除小鼠模型，结合重组MPP6蛋白的体外功能回补实验，证明MPP6通过调控AMPA受体亚基GluA1的膜转运参与海马依赖性学习记忆过程。
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4. **文献名称**: *Reconstitution of the Scribble-MPP6 complex and its role in cell polarity regulation*
**作者**: Navarro, C., et al.
**摘要**: 在昆虫细胞中重组表达MPP6与Scribble蛋白的复合体，通过体外Pull-down和活细胞成像技术揭示二者通过L27结构域介导的相互作用对上皮细胞极性建立的调控机制。
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注：以上文献信息为基于领域知识的概括性模拟，具体研究请以实际文献为准。如需真实文献，建议在PubMed或Web of Science中以“MPP6 recombinant”或“MPP6 protein interaction”为关键词检索。
MPP6 (MAGUK p55 subfamily member 6), also known as PALS2. is a member of the membrane-associated guanylate kinase (MAGUK) protein family. These proteins are characterized by their modular domains, typically including PDZ, SH3. and GuK (guanylate kinase-like) domains, which enable scaffolding functions in cellular signaling and structural organization. MPP6 is primarily recognized for its role in maintaining cell polarity and regulating synaptic protein networks, particularly in neurons and epithelial cells. It interacts with other MAGUK proteins like PALS1 and STAU1. contributing to the formation of multiprotein complexes that stabilize cell junctions and coordinate intracellular trafficking.
Recombinant MPP6 proteins are engineered using expression systems (e.g., E. coli, mammalian cells) to study its biochemical properties, interactions, and functional mechanisms. These purified proteins retain key domains critical for binding partners, enabling in vitro assays such as pull-down experiments, crystallography, or fluorescence-based interaction studies. Research has linked MPP6 to neurological processes, including neurite outgrowth and synaptic plasticity, as well as diseases like cancer and neurodevelopmental disorders. Dysregulation of MPP6 has been observed in glioblastoma and colorectal cancer, suggesting its potential as a therapeutic target.
The development of recombinant MPP6 has advanced structural studies, revealing how its PDZ domain recognizes specific C-terminal motifs of partner proteins, while the GuK domain mediates dimerization. Such insights help decipher its role in cellular homeostasis and disease pathways. Additionally, recombinant MPP6 serves as a tool for drug screening, antibody production, and CRISPR-based functional genomics. Despite progress, questions remain about its tissue-specific isoforms and post-translational modifications, driving ongoing research to fully elucidate its biological and pathological significance.
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